| Project/Area Number |
22580208
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
General fisheries
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| Research Institution | Kitasato University |
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Principal Investigator |
NAKAMURA Osamu 北里大学, 海洋生命科学部, 准教授 (00306648)
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| Co-Investigator(Kenkyū-buntansha) |
TSUTSUI Shigeyuki 北里大学, 海洋生命科学部, 講師 (20406911)
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| Project Period (FY) |
2010 – 2012
|
| Project Status |
Completed (Fiscal Year 2012)
|
| Budget Amount *help |
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2012: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2011: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2010: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
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| Keywords | マナマコ / 体腔球 / リポ多糖 / リポテイコ酸 / ペプチドグリカン / ザイモサン / プロテオーム解析 / C-type レクチン / 生体防御 / 水産学 / ナマコ |
|---|
| Research Abstract |
To investigate acute-phase response proteins in a sea cucumberApostichopus japonicus, the coelomic fluids containing coelomocytes were obtained fromseveral specimens and divided into tubes. Bacterila- or fungi-derived substances suchas LPS and zymosan were added to those coelomic fluids. After incubation for 48h, thesupaernatant of coelomic fluids were subjected to 2-D electrophoresis and compared withthat of controls which were received the buffer only. As a result, many spots that werenot seen in the control were detected in the stimulated fluids. We obtained N-terminalamino acid sequences of several protein spots, and found some unidentified sequences aswell as C-type lectins reported formerly.
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