New understanding of fish regulatory T lymphocytes by LAG-3 molecule analysis

• Project/Area Number: 22580213
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: General fisheries
• Research Institution: Fujita Health University
• Principal Investigator: DIJKSTRA Johannes M. 藤田保健衛生大学, 総合医科学研究所, 助教 (10387681)
• Co-Investigator(Renkei-kenkyūsha): NAKANISHI Teruyuki 日本大学, 生物資源科学部獣医学科, 名誉教授 (00322496)
• Project Period (FY): 2010 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2012: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2011: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2010: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
• Keywords: 魚病 / 魚類免疫学 / 調節性T細胞 / LAG-3 / MHC / 水産学 / 免疫学 / 魚類 / Tリンパ球 / 生体分子 / Th2

Research Abstract

Considerable progress in understanding presumable markers of the fish regulatory T cell system was achieved, including LAG-3, MHC class II, interleukin 15 receptor alpha(IL-15Rα) and interleukin 2 (IL-2).

Research Products

• [Journal Article] Evolution of MHC class II system in teleost fish, the largest vertebrate group, without DM molecules that are critical in tetrapods (2013)
  • Author(s): Dijkstra
  • Journal Title: Proceedings of the National Academy of Sciences of the United States of America Volume: under review
• [Journal Article] Clonal growth of carp (Cyprinus carpio)T cells in vitro : Long-term proliferation of Th2-like cells (2012)
  • Author(s): Yamaguchi, T., Katakura. E., Someya, K., Dijkstra, J.M., Moritomo, T., Nakanishi, T.
  • Journal Title: Fish and shellfish immunology Volume: 34 Issue: 2 Pages: 433-442
  • DOI: 10.1016/j.fsi.2012.11.005
  • Peer Reviewed
• [Journal Article] G6f-like is an ITAM-containing collagen receptor in thrombocytes. (2012)
  • Author(s): Hughes CE, Radhakrishnan UP, Lordkipanidze M, Egginton S, Dijkstra JM, Jagadeeswaran P, Watson SP
  • Journal Title: PLoS One Volume: 7(12) Issue: 12 Pages: e52622-e52622
  • DOI: 10.1371/journal.pone.0052622
• [Journal Article] The expression of CD8alpha discriminates distinct T cell subsets in teleost fish (2011)
  • Author(s): Takizawa, F., Dijkstra, J.M., Kotterba, P., Korytar, T., Kock, H., Kollner, B., Jaureguiberry, B., Nakanishi, T., Fischer, U.
  • Journal Title: Dev.Comp.Immunol. Volume: 35 Issue: 7 Pages: 752-763
  • DOI: 10.1016/j.dci.2011.02.008
  • Peer Reviewed
• [Journal Article] Constitutive high expression of interleukin 4/13 A and GATA-3 in gill and skin of salmonid fishes suggests that these tissues form Th2-skewed immune environments (2011)
  • Author(s): Takizawa, F., E.O.Koppang,; M.Ohtani, T.Nakanishi; K.Hashimoto, U.Fischer; J.M.Dijkstra
  • Journal Title: Mol.Immunol. Volume: 48 Issue: 12-13 Pages: 1360-1368
  • DOI: 10.1016/j.molimm.2011.02.014
  • Peer Reviewed
• [Journal Article] Constitutive high expression of interleukin-4/13A and GATA-3 in gill and skin of salmonid fishes suggests that these tissues form Th2-skewed immune environments (2011)
  • Author(s): Takizawa F
  • Journal Title: Mol.Immunol. Volume: 48 Pages: 1360-68
  • Peer Reviewed
• [Journal Article] Constitutive high expression of interleukin-4/13A and GATA-3 in gill and skin ofsalmonid fishes suggests that these tissues form Th2-skewed immune environments. (2011)
  • Author(s): Takizawa F
  • Journal Title: Mol.Immunol. Volume: (印刷中)
  • Peer Reviewed
• [Journal Article] The expression of CD8alpha discriminates distinct T cell subsets in teleost fish. (2011)
  • Author(s): Takizawa F
  • Journal Title: Dev Comp.Immunol. Volume: (印刷中)
  • Peer Reviewed
• [Journal Article] Salmonid T cells assemble in the thymus, spleen and in novel interbranchial lymphoid tissue. (2010)
  • Author(s): Koppang EO
  • Journal Title: J.Anat. Volume: 217 Pages: 728-739
  • Peer Reviewed
• [Journal Article] A molecule in teleost fish, related with human MHC-encoded G6F, has a cytoplasmic tail with ITAM and marks the surface of thrombocytes and in some fishes also of erythrocytes. (2010)
  • Author(s): Ohashi K
  • Journal Title: Immunogenetics Volume: 62 Pages: 543-559
  • Peer Reviewed
• [Presentation] Thrombocytes and erythrocytes in teleost fish express a surface marker related to mammalian G6F with a cytoplasmic tail bearing an ITAM (2010)
  • Author(s): Dijkstra J
  • Organizer: 第14回 国際免疫学会議
  • Place of Presentation: 神戸
  • Year and Date: 2010-08-23
• [Presentation] MHC in salmonid fish and its linkage associations with disease resistance and social behavior (2010)
  • Author(s): Dijkstra J
  • Organizer: 第9回 国際獣医免疫学シンポジウム
  • Place of Presentation: 東京
  • Year and Date: 2010-08-17
• [Presentation] MHC in salmonid fish and its linkage associations with disease resistance and social behaviour (2010)
  • Author(s): J.M. Dijkstra, M. Ototake, N.Nakanishi, K
  • Organizer: Hashimoto. 9th International Veterinary Immunology Symposium
  • Place of Presentation: Tokyo,Japan
• [Presentation] Thrombocytes and erythrocytes in teleost fish express a surface marker related to mammalian G6F with a cytoplasmic tail bearing an ITAM. (2010)
  • Author(s): J.M. Dijkstra, K. Ohashi, F. Takizawa, H. Toda, T. Nakanishi and K. Hashimoto
  • Organizer: 14th International Congress of Immunology
  • Place of Presentation: Kobe, Japan
• [Remarks]
  • URL: http://www.fujita-hu.ac.jp/ICMS/res03.html
• [Remarks]
  • URL: http://www.fujita-hu.ac.jp/ICMS/res03.html