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Identification of the hemolymph factor controlling the specification and memory in "quasi-immune response" of kuruma shrimp

Research Project

Project/Area Number 22580218
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field General fisheries
Research InstitutionFisheries Research Agency

Principal Investigator

SATOH Jun  独立行政法人水産総合研究センター, 増養殖研究所・病害防除部, 主任研究員 (10443350)

Co-Investigator(Renkei-kenkyūsha) MEKATA Tooru  独立行政法人水産総合研究センター, 増養殖研究所病害防除部, 研究員 (40597944)
Project Period (FY) 2010 – 2012
Project Status Completed (Fiscal Year 2012)
Budget Amount *help
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2012: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2011: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2010: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Keywords水産増殖 / 魚病 / WSSV / WSD / クルマエビ / 免疫様現象 / 組換えタンパク / 生体防御 / 病害防除 / ワクチン / 無脊椎動物 / 組換えタンパク質
Research Abstract

Establishment of the technique to isolate the immune response factor concerned from hemolymph plasma was very difficult, but was able to collect the protein thought to be the immune factor by two-dimensional gel electrophoretic analysis. In addition, the quantitative comparison of immune factor concerned was possible to shown using two-dimensional gel electrophoretic analysis. A part of the immune mechanism of shrimp was investigated, and the judgment of inducing against to WSSV challenge in vaccinated shrimp was enabled in vitro. These results expect to bring the progresses of vaccine development, and the large reduction of the damage by WSSV in shrimp.

Report

(4 results)
  • 2012 Annual Research Report   Final Research Report ( PDF )
  • 2011 Annual Research Report
  • 2010 Annual Research Report

Research Products

(9 results)

All 2013 2012 2011 2010

All Journal Article (4 results) (of which Peer Reviewed: 4 results) Presentation (5 results) (of which Invited: 1 results)

  • [Journal Article] クルマエビのホワイトスポット病WSD(white spot disease)の防除対策に関する研究2012

    • Author(s)
      佐藤 純
    • Journal Title

      水産総合研究センター研究報告

      Volume: 36 Pages: 57-106

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] クルマエビのホワイトスポット病WSD(white spot disease)の防除対策に関する研究2011

    • Author(s)
      佐藤純
    • Journal Title

      水研報

      Volume: 36 Pages: 57-106

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Optimization of WSSV rVP expression in E. coli cells and minimum dose of rVPs for oral vaccination in kuruma shrimp2010

    • Author(s)
      Satoh, J., Kim, H. J., Matsui, T. and Nishizawa, T.
    • Journal Title

      Fish Pathol

      Volume: 45 Pages: 169-174

    • NAID

      10027671458

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Optimization of WSSV rVP expression in E.coli cells and minimum dose of rVPs for oral vaccination in kuruma shrimp2010

    • Author(s)
      佐藤純
    • Journal Title

      魚病研究

      Volume: 45(4) Pages: 169-174

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Presentation] PAV(=WSD)防除のための電解海水を用いたクルマエビ受精卵の洗浄効果2013

    • Author(s)
      佐藤 純
    • Organizer
      日本水産学会
    • Place of Presentation
      東京海洋大学
    • Related Report
      2012 Annual Research Report
  • [Presentation] クルマエビの急性ウイルス血症の防除対策に関する研究2013

    • Author(s)
      佐藤 純
    • Organizer
      日本魚病学会
    • Place of Presentation
      日本大学
    • Related Report
      2012 Annual Research Report
    • Invited
  • [Presentation] PAV(=WSD)防除のための電解海水を用いたクルマエビ受精卵の洗浄効果.2012

    • Author(s)
      佐藤純・米加田徹・森広一郎・乙竹充・吉水守
    • Organizer
      日本水産学会
    • Place of Presentation
      東京海洋大学、東京
    • Related Report
      2012 Final Research Report
  • [Presentation] クルマエビの急性ウイルス血症の防除対策に関する研究2012

    • Author(s)
      佐藤純
    • Organizer
      日本魚病学会
    • Place of Presentation
      日本大学藤沢キャンパス,神奈川
    • Related Report
      2012 Final Research Report
  • [Presentation] クルマエビのホワイトスポット病(WSD:whitespotdisease)に対する組換えタンパク質投与の予防効果について2010

    • Author(s)
      佐藤純
    • Organizer
      節足動物の生体防御に関する研究会
    • Place of Presentation
      増養殖研究所,三重
    • Year and Date
      2010-09-16
    • Related Report
      2012 Final Research Report

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Published: 2010-08-22   Modified: 2019-07-29  

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