Production of recombinant Japanese eel gonadotropins and their roles on the oogenesis

• Project/Area Number: 22580219
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: General fisheries
• Research Institution: Fisheries Research Agency
• Principal Investigator: KAZETO Yukinori 独立行政法人水産総合研究センター, 増養殖研究所・養殖技術部, 主任研究員 (60399996)
• Co-Investigator(Kenkyū-buntansha): GEN Koichiro 水産総合研究センター, 西海区水産研究所, 主任研究員 (80372051)
• Project Period (FY): 2010 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000); Fiscal Year 2012: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2011: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2010: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
• Keywords: 水産学 / ウナギ / 生殖生理 / バイオテクノロジー

Research Abstract

Two recombinant eel gonadotropins (rGTH: rFSH-CTP and rLH-CTP) with long-lasting life were produced in mammalian cells. Administration of these rGTH could induce maturation of immature female eel. Furthermore, ovarian mass and expression of genes related to reproduction, induced by rFSH-CTP were significantly different from those by rLH-CTP, which indicates that two rGTH differentially act in the ovary of eel.

Research Products

• [Journal Article] Molecular characterization and gene expression of Japanese eel (Anguilla japonica) gonadotropin receptors (2012)
  • Author(s): Kazeto Y, Kohara M, Tosaka R, Gen K, Yokoyama M, Miura C, Miura T, Adachi S, Yamauchi K
  • Journal Title: Zoological Sciences Volume: 29 Pages: 204-21
  • NAID: 40019178839
  • Peer Reviewed
• [Journal Article] Molecular characterization and gene expression of Japanese eel (Anguilla japonica) gonadotropin receptors (2012)
  • Author(s): Kazeto Y
  • Journal Title: Zoological Sciences Volume: 29 Issue: 3 Pages: 204-211
  • DOI: 10.2108/zsj.29.204
  • NAID: 40019178839
  • Peer Reviewed
• [Presentation] 組換えウナギ生殖腺刺激ホルモンを恒常発現する哺乳類細胞株の樹立および得られたホルモンの生物活性の検討 (2013)
  • Author(s): 風藤行紀・尾崎雄一・鈴木博史・伊藤理紗・今泉均・野村和晴・田中秀樹・太田博己・田中寿臣・玄浩一郎
  • Organizer: 平成25年度日本水産学会春季大会
  • Place of Presentation: 東京海洋大学
  • Year and Date: 2013-03-28
• [Presentation] ウナギ生殖腺刺激ホルモンの酵素免疫測定系の確立および生体内寿命の長い組換え生殖腺刺激ホルモンの産生 (2012)
  • Author(s): 風藤行紀・深田陽久・鈴木博史・登坂亮太・山口寿哉・奥澤公一・玄浩一郎
  • Organizer: 平成24年度日本水産学会春季大会
  • Place of Presentation: 東京海洋大学
  • Year and Date: 2012-03-24
• [Presentation] 組換えウナギ生殖腺刺激ホルモンを恒常発現する哺乳類細胞株の樹立および得られたホルモンの生物活性の検討
  • Author(s): 風藤行紀
  • Organizer: 平成 25 年度日本水産学会春季大会
  • Place of Presentation: 東京海洋大学