Studies on inhibitory effects of fresh and fermented marine foods on invasion of food related pathogens to enterocyte.

• Project/Area Number: 22580221
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Fisheries chemistry
• Research Institution: Tokyo University of Marine Science and Technology
• Principal Investigator: KUDA Takashi 東京海洋大学, 海洋科学技術研究科, 准教授 (00290081)
• Project Period (FY): 2010 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2012: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2011: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2010: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
• Keywords: Lactobacillus plantarum / Leuconostoc mesenteroides / Listeria monocytogenes / Salmonella Typhimurium / なれずし / マコンブ / 腸管上皮細胞 Caco-2 / Listeria / Leuconostoc / Caco-2 / RAW264.7 / IBDマウス / マウス / 脾臓 / 腸管上皮細胞Caco-2

Research Abstract

In this study, to clarify the inhibitory effects of marine foods on Listeria infection, we investigated with human enterocyte like Caco-2 cells and A/J mice. Leuconostoc mesenteroides 1RM3 suppressed the invasion and infection of Listeria. The inhibitory effect of 1RM3 might be related with its immune promote and anti-inflammatory activities.

Research Products

• [Journal Article] FLX pyrosequencing analysis of the effects of the brown-algalfermentable polysaccharides alginate and laminaran on rat cecal microbiotas (2013)
  • Author(s): Kuda, T., Yazaki, T., Takahashi, H.,Kimura, B
  • Journal Title: Appled and Environmental Microbiology Volume: 79 Issue: 4 Pages: 860-866
  • DOI: 10.1007/s12010-012-9831-4
  • Peer Reviewed
• [Journal Article] FLX pyrosequencing analysis of the effects of the brown-algal fermentable polysaccharides alginate and laminaran on rat cecal microbiotas (2013)
  • Author(s): An, C., Kuda, T., Yazaki, T., Takahashi, H., Kimura, B
  • Journal Title: Appled and Environmental Microbiology Volume: 79 Issue: 3 Pages: 860-866
  • DOI: 10.1128/aem.02354-12
  • Peer Reviewed
• [Journal Article] Diet-induced changes in alginate- and laminaran-fermenting bacterial levels in the caecal contents of rats (2013)
  • Author(s): An, C., Yazaki, T., Takahashi, H., Kuda, T., & Kimura, B
  • Journal Title: Journal of Functional Foods Volume: 5 Issue: 1 Pages: 389-394
  • DOI: 10.1016/j.jff.2012.11.011
  • Peer Reviewed
• [Journal Article] scavengingControlH2O2H2O2+1RM3capacities of saba-narezushi, Japanese図3過酸化水素のマウスマクロファージ細 胞への毒性に対する Ln. mesenteroides1RM3 の保護作用fermented chub mackerel, and its lacticacid bacteria (2012)
  • Author(s): Kanno, T., Kuda, T., Choa, A., Takahashi, H., & Kimura, B. Radical
  • Journal Title: LWT-Food Science and Technology Volume: 47 Pages: 25-30
  • Peer Reviewed
• [Journal Article] Inhibitory effects of Leuconostoc mesenteroides 1RM3 isolated from narezushi, a fermented fish with rice, on Listeria monocytogenes infection to Caco-2 cells and A/J mice (2012)
  • Author(s): Nakamura, S., Kuda, T., An, C., Kanno, T., Takahashi, H., & Kimura, B
  • Journal Title: Anaerobe Volume: 18 Issue: 1 Pages: 19-24
  • DOI: 10.1016/j.anaerobe.2011.11.006
  • Peer Reviewed
• [Journal Article] Radical scavenging capacities of saba-narezushi, Japanese fermented chub mackerel, and its lactic acid bacteria (2012)
  • Author(s): Kanno T., Kuda T., An C., Takahashi H., Kimura B.
  • Journal Title: LWT-Food Science and Technology Volume: 47 Issue: 1 Pages: 25-30
  • DOI: 10.1016/j.lwt.2012.01.007
  • Peer Reviewed
• [Presentation] さばのなれずしより分離した乳酸菌の抗酸化性および抗炎症性 (2012)
  • Author(s): 菅野友美・安チョア・高橋肇・木村凡・久田孝
  • Organizer: 日本乳酸菌学会大会
  • Place of Presentation: つくば
  • Year and Date: 2012-07-13
• [Presentation] Caco-2およびマウスにおけるリステリア侵入および感染に対するマコンフ葉状体および付着部(ガニアシ)乾物の影響 (2012)
  • Author(s): 中村信介・安チョア・高橋肇・木村凡・西澤信・久田孝
  • Organizer: 日本水産学会
  • Place of Presentation: 東京海洋大学品川キャンパス(東京都)
  • Year and Date: 2012-03-29
• [Presentation] Caco-2およびマウスにおけるリステリア侵入および感染に対するマコンブ葉状体および付着部(ガニアシ)乾物の影響 (2012)
  • Author(s): 中村信介・安チョア・高橋肇・木村凡・西澤信・久田孝
  • Organizer: 日本水産学会春季大会
  • Place of Presentation: 東京
  • Year and Date: 2012-03-26
• [Presentation] 褐藻類中の発酵性多糖類がラットの腸内フローラに及ぼす影響‐次世代シーケンサーを用いた解析‐ (2011)
  • Author(s): 安チョア・久田孝・矢崎孝寛・木村凡
  • Organizer: 日本食品微生物学会学術総会
  • Place of Presentation: 東京
  • Year and Date: 2011-10-06
• [Presentation] さばのなれずしより分離したLeuconostoc mesenteroidesによるCaco-2 細胞およびA/Jマウスにおけるリステリアの侵入および感染の抑制 (2011)
  • Author(s): 中村伸介・菅野友美・高橋肇・木村凡・久田孝
  • Organizer: 日本乳酸菌学会大会
  • Place of Presentation: 大阪
  • Year and Date: 2011-07-12
• [Presentation] さばのなれずしより分離したLeuconostoc mesenteroidesによるCaco-2細胞およびA/Jマウスにおけるリステリアの侵・および感染の抑制 (2011)
  • Author(s): 中村信介・菅野友美・高橋肇・木村凡・久田孝
  • Organizer: 日本乳酸菌学会
  • Place of Presentation: 関西大学千里山キャンパス(大阪府)
  • Year and Date: 2011-07-12
• [Presentation] さばのなれずしより分離した乳酸菌の抗酸化性および抗炎症性
  • Author(s): 菅野友美
  • Organizer: 日本乳酸菌学会
  • Place of Presentation: つくば国際会議場
• [Remarks] 本年度の成果は次年度の学会で報告予定。また下記論文を投稿中 Inhibition of Listeria monocytogenes invasion and infection to Caco-2 cells and A/J mice by Leuconostoc mesenteroides lRM3 isolated from narezushi, a salted and long-fermented fish with cooked rice. Food Microbiology