Mechanism of gene expression regulation of anti-oxidant enzymeswith oxidative stress in fishes - Molecular cell biological approach -

• Project/Area Number: 22580224
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Fisheries chemistry
• Research Institution: Nagasaki University
• Principal Investigator: OSATOMI Kiyoshi 長崎大学, 水産・環境科学総合研究科, 教授 (40253702)
• Co-Investigator(Kenkyū-buntansha): HARA Kenji 長崎大学, 水産・環境科学総合研究科, 教授 (10039737) ; ODA Tatsuya 長崎大学, 水産・環境科学総合研究科, 教授 (60145307) ; KANAI Kinya 長崎大学, 水産・環境科学総合研究科, 教授 (40145222)
• Project Period (FY): 2010 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2012: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2011: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2010: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: 抗酸化酵素 / プロモーター / 酸化ストレス / 魚病細菌 / 細胞培養系

Research Abstract

In this study, we examined the effects of extracellular products(ECP) from Edwardsiella tarda (E.tarda) strains on macrophages in terms of the induction of NO and TNF-α. ECP from virulent strain (NUF251) of E.tarda stimulated macrophages to induce NO and TNF-αproductions in a concentration-dependent manner. Proteomic analysis of ECP from E.tarda strains suggested that NUF251-specific protein, which has sequence homology with flagellin, is present in NUF251-ECP as a main component.

Research Products

• [Journal Article] Purification molecular cloning, and some properties of a manganese-containing superoxide dismutase from Japanese flounder (Paralichthys olivaceus) (2011)
  • Author(s): Y. Wang, K. Osatomi, Y. Nagatomo, A. Yoshida, K. Hara.
  • Journal Title: Comparative Biochemistry and Physiology Part B Volume: 158 Pages: 289-96
  • NAID: 120006984788
  • Peer Reviewed
• [Journal Article] Purification, molecular cloning, and some properties of a manganese-containing superoxide disumutase from Japanese flounder (Paralichthys olivaceus) (2011)
  • Author(s): Yajun Wang
  • Journal Title: Comparative Biochemistry and Physiology, Part B Volume: 158 Pages: 289-296
  • Peer Reviewed
• [Journal Article] Extracellular products from virulent strain of Edwardsiella tarda stimulate mouse macrophages (RAW264.7) to produce nitric oxide (NO) and tumor necrosis factor (TNF)-α. (2010)
  • Author(s): Y. Wang, K. Osatomi, A. Yoshida, X. Liang, K. Kanai, T. Oda, K. Hara
  • Journal Title: Fish&Shellfish Immunology Volume: 29 Pages: 778-85
  • Peer Reviewed
• [Presentation] Studies on structure, function and pathological significance of antioxidant enzymes in fishes. (2012)
  • Author(s): K. Osatomi
  • Organizer: International Conference on Food Science
  • Place of Presentation: Dalian, China.
  • Year and Date: 2012-08-30
• [Presentation] Studies on structure, function and pathological significance of antioxidant enzymes in fishes. (2012)
  • Author(s): K. Osatomi
  • Organizer: 2012 International Conference on Food Science
  • Place of Presentation: Dalian, China
  • Invited
• [Presentation] Edwardsiella tarda菌体外産生物質暴露に伴うマクロファージ系細胞株の応答 (2010)
  • Author(s): 王 亜軍
  • Organizer: 2010年度日本水産学会秋季大会
  • Place of Presentation: 京都大学吉田キャンパス
  • Year and Date: 2010-09-23