| Project/Area Number |
22580226
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Fisheries chemistry
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| Research Institution | Yokohama City University |
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Principal Investigator |
OZEKI Yasuhiro 横浜市立大学, 大学院・生命ナノシステム科学研究科, 教授 (70275022)
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| Co-Investigator(Kenkyū-buntansha) |
YASUMITSU Hidetaro 横浜市立大学, 生命ナノシステム科学研究科, 教授 (10182346)
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| Project Period (FY) |
2010 – 2012
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| Project Status |
Completed (Fiscal Year 2012)
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| Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2012: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2011: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2010: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | レクチン / MytiLec / 一次構造 / グロボトリオース / ABCトランスポーター / SUEL/ラムノース結合レクチンファミリー / 糖鎖 / フロンタルアフィニティクロマトグラフィー / アポトーシス / 多剤耐性トランスポーター / バーキットリンパ腫細胞 / フロンタルアフィニティークロマトグラフィー / 腹足類 / 棘皮動物 / N-アセチルラクトサミン / 細胞死 / 有柄亜門 / 軟体動物 |
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| Research Abstract |
A17kDa α-galactoside-binding lectin,MytiLec purified from the Mediterranean mussel was found to have a novel triple-tandem repeat structure. It recognized globotriose (Gb3)and appeared strong cell static activity againstGb3 expressing lymphoma Raji cells. Another α-galactoside-binding lectin, SAL,a member of SUEL/rhamnose-binding lectin family isolated from catfish eggdid not influence the growth of Raji cells, though it down-regulated the expression levels of the multidrug-resistanttransporter gene. Combining these results, it became clear that even lectins having same oligosaccharide binding specificity, can transmit different signals to cells according to the variations of their multivalency and dissociation constants. This new finding will be usefulto study the cellular functions of these lectins to regulate cell proliferation.
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