Studies on thick filament formation during myofibrillogenesis

• Project/Area Number: 22580301
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Zootechnical science/Grassland science
• Research Institution: Hokkaido University
• Principal Investigator: HATTORI Akihito 北海道大学, 名誉教授 (50125027)
• Co-Investigator(Kenkyū-buntansha): OJIMA Koichi 畜産草地研究所, 畜産物研究領域, 主任研究員 (60415544)
• Project Period (FY): 2010 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2011: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2010: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
• Keywords: 骨格筋 / ミオシン / 太いフィラメント / 筋原線維

Research Abstract

Thick filaments are composed of more than 300 myosin molecules in myofibrils. In this study, we found that the light meromyosin (LMM), which is one of myosin heavy chain (Myh) domains, was essential to form thick filaments in cultured skeletal muscle cells. Furthermore, to form thick filaments of LMM was facilitated by the presence of the subfragment-2 (S2), although solely expressed S2 did not form thick filaments, suggesting that the S2 may regulate thick filament formation.

Research Products

• [Presentation] 骨格筋細胞におけるミオシン分子の太いフィラメント形成能 (2013)
  • Author(s): 尾嶋孝一,中島郁世,大江美香,室谷進,服部昭仁,西邑隆徳
  • Organizer: 日本畜産学会第116回大会
  • Place of Presentation: 広島市安田女子大学
  • Year and Date: 2013-03-30
• [Presentation] 筋原線維の太いフィラメント内でミオシン分子はダイナミックに置き換わっている (2013)
  • Author(s): 安川裕也,尾嶋孝一,若松純一,服部昭仁,西邑隆徳
  • Organizer: 日本畜産学会第116回大会
  • Place of Presentation: 広島市安田女子大学
  • Year and Date: 2013-03-28
• [Presentation] 骨格筋細胞におけるミオシン分子の太いフィラメント形成能 (2013)
  • Author(s): 尾嶋孝一, 中島郁世, 大江美香, 室谷進, 服部昭仁, 西邑隆徳
  • Organizer: 日本畜産学会第116回大会
  • Place of Presentation: 安田女子大学（広島市）
• [Presentation] 筋原線維の太いフィラメント内でミオシン分子はダイナミックに置き換わっている (2013)
  • Author(s): 安川裕也, 尾嶋孝一, 若松純一, 服部昭仁, 西邑隆徳
  • Organizer: 日本畜産学会第116回大会
  • Place of Presentation: 安田女子大学（広島市）
• [Presentation] Myosin dynamicsin cultured skeletal muscle cells. (2012)
  • Author(s): Yasukawa, Y., Ojima, K., Wakamatsu, J.-i.,Hattori, A., and Nishimura, T.
  • Organizer: 2012 Annual Meeting of the American Society for Cell Biology
  • Place of Presentation: The Moscone Center, San Francisco, USA.
  • Year and Date: 2012-12-17
• [Presentation] The impact of each domain of myosin heavy chain molecule on forming thick filaments in muscle and non-muscle cells. (2012)
  • Author(s): Ojima, K., Nakajima, I., Oe, M., Muroya, S.,Nishimura, T., and Hattori, A.
  • Organizer: 2012 Annual Meeting of the American Society for Cell Biology
  • Place of Presentation: The Moscone Center, San Francisco, USA.
  • Year and Date: 2012-12-17
• [Presentation] Myosin dynamics in cultured skeletal muscle cells (2012)
  • Author(s): Y. Yasukawa, K. Ojima, J. Wakamatsu, A. Hattori, T. Nishimura
  • Organizer: 2012 Annual Meeting of the American Society for Cell Biology
  • Place of Presentation: San Francisco convention center (San Francisco, USA)
• [Presentation] The impact of each domain of myosin heavy chain molecule on forming thick filaments in muscle and non-muscle cells. (2012)
  • Author(s): K. Ojima, I. Nakajima, M. Oe, S. Muroya, T. Nishimura, A. Hattori.
  • Organizer: 2012 Annual Meeting of the American Society for Cell Biology
  • Place of Presentation: San Francisco convention center (San Francisco, USA)