Establishment of method for rumen methane mitigation by hydrogen peroxide

• Project/Area Number: 22580302
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Zootechnical science/Grassland science
• Research Institution: Obihiro University of Agriculture and Veterinary Medicine
• Principal Investigator: NISHIDA Takehiro 帯広畜産大学, 畜産学部, 准教授 (70343986)
• Co-Investigator(Kenkyū-buntansha): TAKAHASHI Junichi 帯広畜産大学, その他部局等, 名誉教授 (20111198)
• Project Period (FY): 2010 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000); Fiscal Year 2012: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2011: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2010: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
• Keywords: 栄養・飼養 / メタン抑制 / 過酸化水素 / in vivo / メタン発生量 / ORP

Research Abstract

Hydrogen peroxide was introduced into sheep to become 0.5mM, 0.75mM and 1.0mM in their rumen. Methane production from the rumen were 22.9, 37.4 and 53.4% lower than additive free sheep, respectively. Sodium percarbonate (granularity) were added 0.5, 1.0 and 1.5mM in rumen juice from cattle. Methane production from the culture fluid were 26.5, 22.4 and 32.7% lower than additive free culture, respectively. Sodium percarbonate were fed 0.5, 1.0, 1.5 and 2.0mM in sheep and ruminal methane production were lower than additive free sheep in a dose-dependent manner.

Research Products

• [Journal Article] Effect of mixed microbial culture treatment on the nutritive value of coffee, green tea and oolong tea residues and the effect of the fermented residues on in vitro rumen fermentation. (2012)
  • Author(s): Senevirathne N. D., Okamoto T., Takahashi J., Umetsu K., Nishida T.
  • Journal Title: International Journal of Bioscience, Biochemistry and Bioinformatics Volume: 2 Pages: 349-353
  • DOI: 10.7763/ijbbb.2012.v2.130
• [Journal Article] Effect of secondary metabolites in beverage industry residues on rumen methane emission (2012)
  • Author(s): Santoso, B., Asa, R., Nishida, T., Takahashi, J.
  • Journal Title: Greenhouse gases and sustainable animal agriculture for developing countries Volume: 79 Pages: 29-34
• [Journal Article] Effects of Protease-resistant Antimicrobial Substances Produced by Lactic Acid Bacteria on Rumen Methanogenesis (2010)
  • Author(s): Reina Asa, A. Tanaka, A. Uehara, I. Shinzato, Y. Toride, N. Usui, K. Hirakawa, J. Takahashi
  • Journal Title: Asian-Aust. J. Anim. Sci. Volume: 23 Pages: 700-707
• [Journal Article] Effects of Protease-resistant Antimicrobial Substances Produced by Lactic Acid Bacteria on Rumen Methanogenesis (2010)
  • Author(s): Reina Asa, A., Tanaka, A., Uehara, I., Shinzato, Y., Toride, N., Usui, K., Hirakawa, J., Takahashi
  • Journal Title: Asian-Aust.J.Anim.Sci. Volume: 23 Pages: 700-707
  • Peer Reviewed
• [Presentation] 無線伝送式ルーメンpHメータの泌乳牛での応用例 (2013)
  • Author(s): 西田武弘, 嘉納梢, 水口人史, 佐藤繁
  • Organizer: 第116回日本畜産学会
  • Place of Presentation: 安田女子大学，広島
• [Presentation] 乳酸菌の生成する抗菌性物質のルーメンメタン抑制効果 (2012)
  • Author(s): O'Brien Martin,橋本哲平,西田武弘,田中尚人,岡田早苗,高橋潤一
  • Organizer: 第113回日本畜産学会
  • Place of Presentation: 名古屋大学
  • Year and Date: 2012-03-29
• [Presentation] The effectiveness of Alcaligenes faecalis and nitrate to suppress in vitro rumen methanogenesis (2012)
  • Author(s): O'Brien Martin, Shoda M., Nishida T., Takahashi J.
  • Organizer: 第113回日本畜産学会
  • Place of Presentation: 名古屋大学
  • Year and Date: 2012-03-29
• [Presentation] Effect of residual of coffee, green tea, and oolong tea treated with mixed microbes on rumen fermentation (2012)
  • Author(s): Senevirathne N., Okamoto T., Nishida T., Takahashi J.
  • Organizer: 第113回日本畜産学会
  • Place of Presentation: 名古屋大学
  • Year and Date: 2012-03-29
• [Presentation] The effectiveness of Alcaligenes faecalis and nitrate to suppress in vitrorumen methanogenesis (2012)
  • Author(s): O'Brien Martin, Shoda M., Nishida T., Takahashi J.
  • Organizer: 第113日本畜産学会
  • Place of Presentation: 名古屋大学
  • Year and Date: 2012-03-29
• [Presentation] Nutritive value, energy metabolism, rumen fermentation and methane emission of fermented spent coffee grounds as a replacement of hay in total mixed ration fed Corriedale sheep. (2012)
  • Author(s): Senevirathne N. D., Santoso B., Okamoto T., Takahashi J., Umetsu K., Nishida T.
  • Organizer: The 15th AAAP Animal Science Congress
  • Place of Presentation: Thammasart University, Thailand
• [Presentation] Suppressing in vitro rumen methanogenesis with Alcaligenes faecalis and a low concentration of nitrate (2012)
  • Author(s): O’Brien Martin, Shoda M., Nishida T., Takahashi J.
  • Organizer: The 15th AAAP Animal Science Congress
  • Place of Presentation: Thammasart University, Thailand
• [Presentation] Mitigation of rumen methane emission from Thai native cattle by artificial fiber administration (2012)
  • Author(s): Angthong W., Pholsen P., Namsilee R., Udchachon S., Takahashi J. Nishida T.
  • Organizer: The 15th AAAP Animal Science Congress
  • Place of Presentation: Thammasart University, Thailand
• [Presentation] Effects of soybean hull feeding on the feed intake, chewing time and milk production (2012)
  • Author(s): S. Ando, M. Ishida and Nishida T.
  • Organizer: The 15th AAAP Animal Science Congress
  • Place of Presentation: Thammasart University, Thailand
• [Presentation] 十勝における農業副産物の飼料利用 (2012)
  • Author(s): 西田武弘
  • Organizer: 第1回北海道畜産草地学会
  • Place of Presentation: 北海道大学，札幌
• [Presentation] The effectiveness of Alcaligenes faecalis to reduce nitrite accumulation when nitrate is used to suppress in vitro rumen methanogenesis (2011)
  • Author(s): O' Brien, M., M. Shoda, T. Nishida, J. Takahashi
  • Organizer: The 8th Japan-Korea-China Joint Symposium on Rumen Metabolism and Physiology
  • Place of Presentation: 札幌, 北海道
  • Year and Date: 2011-10-17
• [Presentation] The effectiveness of Alcaligenes faecalis to reduce nitrite accumulation when nitrate is used to suppress in vitro rumen methanogenesis (2011)
  • Author(s): O'Brien, M., M.Shoda, T.Nishida, J.Takahashi
  • Organizer: The 8th Japan-Korea-China joint symposium on rumen metabolism and physiology
  • Place of Presentation: 札幌,北海道
  • Year and Date: 2011-10-17
• [Presentation] Thermophilic anaerobic co-digestion of synthetic waste and dairy cattle manure (2011)
  • Author(s): Moriya, Y., T. Yamashiro, C. Ying, A. Fetra, W. Rui, Z. Shasha, M. Iwasaki, J. Takahashi, T. Nishida, M. Saito, A. Hirata, S. Ogura, I. Ihara and K. Umetsu
  • Organizer: The third conference on Sustainable Animal Agriculture for Developing Countries (SAADC 2011)
  • Place of Presentation: Nakhon Ratchasima, Thailand
  • Year and Date: 2011-07-29
• [Presentation] Thermophilic anaerobic co-digestion of synthetic waste and dairy cattlemanure (2011)
  • Author(s): Moriya, Y., T.Yamashiro, C.Ying, A.Fetra, W.Rui, Z.Shasha, M.Iwasaki, J.Takahashi, T.Nishida, M.Saito, A.Hirata, S.Ogura, I.Ihara, K.Umetsu
  • Organizer: The third conference on Sustainable Animal Agriculture for Developing Countries (SAADC 2011)
  • Place of Presentation: Nakhon Ratchasima, Thailand
  • Year and Date: 2011-07-29
• [Presentation] Effects of ruminal dosing of mechanical stimulating brush on methane emission from rumen in dry cows (2011)
  • Author(s): Chaokaur, A., S. Kempaka, T. Matsumoto, T. Okamoto, J. Takahashi and T. Nishida
  • Organizer: The third conference on Sustainable Animal Agriculture for Developing Countries (SAADC 2011)
  • Place of Presentation: Nakhon Ratchasima, Thailand
  • Year and Date: 2011-07-28
• [Presentation] Effects of ruminal dosing of mechanical stimulating brush on methaneemission from rumen in dry cows (2011)
  • Author(s): Chaokaur, A., S.Kempaka, T.Matsumoto, T.Okamoto, J.Takahashi, T.Nishida
  • Organizer: The third conference on Sustainable Animal Agriculture for Developing Countries (SAADC 2011)
  • Place of Presentation: Nakhon Ratchasima, Thailand
  • Year and Date: 2011-07-28
• [Presentation] Effects of secondary metabolites in the residues from beverage industries on rumen methane emission (2011)
  • Author(s): Santoso, B., R. Asa, T. Nishida and J. Takahashi
  • Organizer: The third conference on Sustainable Animal Agriculture for Developing Countries (SAADC 2011)
  • Place of Presentation: Nakhon Ratchasima, Thailand
  • Year and Date: 2011-07-26
• [Presentation] Effect of PEG and KMnO4 on In-vitro fermentation and rumen degradation characteristics of tannin rich tree fodders (2011)
  • Author(s): Senevirathne, N. D., Jayawardana, V. P., Nishida, T., Takahashi, J.
  • Organizer: The third conference on Sustainable Animal Agriculture for Developing Countries (SAADC 2011)
  • Place of Presentation: Nakhon Ratchasima, Thailand
  • Year and Date: 2011-07-26
• [Presentation] Effect of blended microbial feed additives on nutritive value of sugar beet tops with stem and stub of adzuki beans after long-term preservation (2011)
  • Author(s): Nishida, T., H. Mori, A. Tanaka, K. Umetsu, T. F. Hung, H. Itoh, I. Sakaida, J. Takahashi
  • Organizer: The third conference on Sustainable Animal Agriculture for Developing Countries (SAADC 2011)
  • Place of Presentation: Nakhon Ratchasima, Thailand
  • Year and Date: 2011-07-26
• [Presentation] Effect of Hydrogen Peroxide on In Vitro Ruminal Fermentations (2010)
  • Author(s): K. Hirakawa, T. Matsumoto, R. Asa-Morikawa, H. Nishigaki, Y. Mihara, J. Takahashi
  • Organizer: 4th International Conference on Greenhouse Gases and Animal Agriculture
  • Place of Presentation: Banff, Alberta, Canada
  • Year and Date: 2010-10-05
• [Presentation] Effect of Hydrogen Peroxide Administration on Rumen Fermentations of Sheep (2010)
  • Author(s): K. Hirakawa, T. Matsumoto, H. Nishigaki, R. Asa-Morikawa, Y. Mihara, T. Nishida, J. Takahashi
  • Organizer: 4th International Conference on Greenhouse Gases and Animal Agriculture
  • Place of Presentation: Banff, Alberta, Canada
  • Year and Date: 2010-10-05