| Budget Amount *help |
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2012: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2011: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2010: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Research Abstract |
The author prepared an IgG-rich fraction specific to Saccaromyces (S.) pastorianus (milk antibody) from colostrum produced by a S. pastorianus-immunized cow. The allergic symptoms indicated as nose rubbing events of miceNC/Nga mice and ear thickness of BALB/c mice given a diet containing amixture of S. pastorianus and milk antibody (mixture-added diet) were significantly lower than in those given the S. pastorianus and milk antibody-free diet (control diet). The number of FcεRIα+CD117+ cells and mRNA expression of STIM1 were significantly lower in spleens of NC/Nga mice given the mixture-added diet than in those given the control diet. The mRNA expression of all subunit chains of IgE receptor, i.e. FcεRIα, FcεRIβ and FcεRIγ and proteins such as STIM1, BTK and ITK relating to degranulation was significantly reduced in C3H/HeN mouse spleen cell cultures with a mixture of S.pastorianus and milk antibody than in those without the S. pastorianus and/or milk antibody. On the other hand, only minimal acute toxicity was observed in ICR mice given 2,000 mg of S. pastorianusand/or milk antibody/g body weight. These results suggest that the mixture of S. pastorianus and milk antibody represents a safety anti-allergic food material.
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