| Budget Amount *help |
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2012: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2011: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2010: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Research Abstract |
The present study was conducted to identify a IgY receptor contributing IgY uptake into avian egg yolks. The uptakes of recombinant IgY mutants into egg yolks were determined when intravenously injected into birds. The results showed that substitution of tyrosine residue at 363 of IgY markedly reduced IgY uptake into egg yolks. Since critical hurdle of Y363A mutant transport into egg yolks existed in the innermost layer of avian ovarian follicles, the inner most layer was used for isolation and identification of the IgY receptor. Seven candidate proteins for the IgY receptor was isolated from plasma membrane fraction of the inner most layer of ovarian follicles, which included yolk-precursor protein, vitellogenin. Further studies are necessary to clarify the precise mechanism of IgY uptake mediated by vitellogenin.
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