Research on the mechanism of improvement of nitrogen utilization by the feeding of fermentable sugars in rabbits

• Project/Area Number: 22580309
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Zootechnical science/Grassland science
• Research Institution: Okayama University
• Principal Investigator: SAKAGUCHI Ei 岡山大学, 大学院・環境生命科学研究科, 教授 (40170584)
• Project Period (FY): 2010 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000); Fiscal Year 2012: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000); Fiscal Year 2011: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000); Fiscal Year 2010: ¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000)
• Keywords: ウサギ / 発酵性糖質 / 窒素代謝 / 15^N-尿素 / 盲腸内微生物 / 食糞 / 血中尿素 / 難消化性糖質 / 15N-尿素 / ^<15>N-尿素 / 窒素利用 / 盲腸内バクテリア / 腸内微生物 / 盲腸 / 腸内発酵

Research Abstract

It was shown in this study that feeding of indigestible sugarsfuructooligosaccharide and mannitol which escape gastrointestinal digestion improved the utilization of dietary protein and this should be resulted from the following effects: Indigestible sugars stimulated cecal bacterial proliferation using blood urea as a nitrogen source and the bacterial protein increased in the cecum was ingested by rabbits as nutritional quality protein. These findings must be put to practical use for the improvementof production efficiency and for reduction of nitrogen excretion.

Research Products

• [Journal Article] Effect of D-mannitol on Nitrogen Retention, Fiber Digestibility, and Digesta Transit Time in Adult Rabbits (2013)
  • Author(s): Xiao Min, Xiao LI, Shouko Hiura, Kiyonori Kakwasaki, Jin Xiao, Ei Sakaguchi
  • Journal Title: Animal Science Journal Volume: 84 Issue: 7 Pages: 551-555
  • DOI: 10.1111/asj.12041
  • Peer Reviewed
• [Journal Article] Effect of fructooligosaccharide on nitrogen utilization in guinea pigs (2013)
  • Author(s): Kiyonori Kawasaki, Xiao Min, Ai Nishiyama, Ei Sakaguchi.
  • Journal Title: Animal Science Journal Volume: 84 Pages: 328-333
  • Peer Reviewed
• [Journal Article] Transfer of blood urea nitrogen to cecal microbial nitrogen is increased by mannitol feeding in growing rabbits fed timothy hay diet (2012)
  • Author(s): Xiao L., Xiao M., Jin X, Kawasaki K., Ohta N., Sakaguchi E
  • Journal Title: Animal Volume: 6 Issue: 11 Pages: 1757-1763
  • DOI: 10.1017/s1751731112000754
  • Peer Reviewed
• [Journal Article] Utilization of dietary urea nitrogen is stimulated by D-mannitol feeding in rabbits (2012)
  • Author(s): Xiao L., Xiao M., Jin X., Kawasaki K., Ohta N. and Sakaguchi E.
  • Journal Title: Animal Science Journal Volume: 83 Pages: 605-609
  • Peer Reviewed
• [Journal Article] Utilization of dietary urea nitrogen is stimulated by D-mannitol feeding in rabbits (2012)
  • Author(s): Xiao L., Xiao M., Jin X, Kawasaki K., Ohta N., Sakaguchi E.
  • Journal Title: Animal Science Journal Volume: (印刷中) Issue: 8 Pages: 605-609
  • DOI: 10.1111/j.1740-0929.2011.01004.x
  • Peer Reviewed
• [Journal Article] Effect of indigestible sugars on nitrogen utilization in adult rabbits (2011)
  • Author(s): Xiao Li, Xiao Min, Tsuzuki Y. and Sakaguchi E
  • Journal Title: Animal Science Journal Volume: 82 Issue: 2 Pages: 296-301
  • DOI: 10.1111/j.1740-0929.2010.00849.x
  • NAID: 10030376509
  • Peer Reviewed
• [Journal Article] ウサギの窒素利用性をフラクトオリゴ糖が亢進する (2010)
  • Author(s): 都築勇太
  • Journal Title: 草食実験動物 Volume: 33号 Pages: 19-26
  • Peer Reviewed
• [Presentation] フラクトオリゴ糖(FOS)が成熟ウサギの血中尿素動態に及ぼす影響 (2013)
  • Author(s): 暁敏,川崎淨教,金曉,坂口英
  • Organizer: 日本畜産学会第116回大会
  • Place of Presentation: 安田女子大学(広島市)
• [Presentation] フラクトオリゴ糖（FOS）が成熟ウサギの血中尿素動態に及ぼす影響 (2013)
  • Author(s): 暁敏，川崎淨教，金曉，（坂口英）
  • Organizer: 日本畜産学会第116回大会
  • Place of Presentation: 安田女子大学（広島市）
• [Presentation] Effect of fructooligosaccaride on nitrogen utilization in growing rabbits fed on different dietary protein levels (2012)
  • Author(s): Naoko Ota, Xiao Li, Xiao Min, Ei Sakaguchi
  • Organizer: Asian-Australasian Animal Production (AAAP) Congress
  • Place of Presentation: バンコク(タイ)
• [Presentation] Transfer of blood urea nitrogen to cecal microbial nitrogen is increased by fructooligosaccharide feeding in guinea pigs (2012)
  • Author(s): Kiyonori Kawasaki, Xiao Min, Xiao Li, EnaHasegawa, Ei Sakaguchi
  • Organizer: Ninth Biennial Symposium of the Comparative Nutrition Society
  • Place of Presentation: パシフィックグローブ(カリフォルニア, USA)
• [Presentation] Fermentable sugar fructooligosaccharide increases nitrogen utilization through cecotrophy in rabbits (2012)
  • Author(s): Yuta Tsuzuki, Xiao Li, Ei Sakaguchi
  • Organizer: Ninth Biennial Symposium of the Comparative Nutrition Society
  • Place of Presentation: パシフィックグローブ(カリフォルニア, USA)
• [Presentation] Effect of fructooligosaccaride on nitrogen utilization in growing rabbits fed on different dietary protein levels (2012)
  • Author(s): Naoko Ota, Xiao Li, Xiao Min and (Ei Sakaguchi)
  • Organizer: Asian-Australasian Animal Production (AAAP) Congress
  • Place of Presentation: バンコク（タイ国）
• [Presentation] Transfer of blood urea nitrogen to cecal microbial nitrogen is increased by fructooligosaccharide feeding in guinea pigs (2012)
  • Author(s): Kiyonori Kawasaki, Xiao Min, Xiao Li, Ena Hasegawa, (Ei Sakaguchi)
  • Organizer: Ninth Biennial Symposium of the Comparative Nutrition Society
  • Place of Presentation: パシフィックグローブ（カリフォルニア，アメリカ合衆国）
• [Presentation] Fermentable sugar fructooligosaccharide increases nitrogen utilization through cecotrophy in rabbits (2012)
  • Author(s): Yuta Tsuzuki, Xiao Li, （Ei Sakaguchi）
  • Organizer: Ninth Biennial Symposium of the Comparative Nutrition Society
  • Place of Presentation: パシフィックグローブ（カリフォルニア，アメリカ合衆国）
• [Presentation] Fermentable sugar increases transfer of blood urea N to cecal microbial N in growing rabbits (2011)
  • Author(s): Xiao L., Xiao M., 坂口英
  • Organizer: 第61回関西畜産学会大会
  • Place of Presentation: 岡山大学
  • Year and Date: 2011-09-13
• [Presentation] 飼料条件の違いが50日齢のウサギにおけるフラクトオリゴ糖の窒素蓄積効率に及ぼす効果に対する影響 (2011)
  • Author(s): 太田奈緒子, 曉敏, 曉利, 川崎淨教, 坂口英
  • Organizer: 第61回関西畜産学会大会
  • Place of Presentation: 岡山大学
  • Year and Date: 2011-09-13
• [Presentation] ウサギの窒素利用に及ぼす異なる難消化性糖質の影響 (2011)
  • Author(s): 曉敏, 曉利, 太田奈緒子, 肥田愛澄, 坂口英
  • Organizer: 第61回関西畜産学会大会
  • Place of Presentation: 岡山大学
  • Year and Date: 2011-09-13
• [Presentation] Fermentable sugar increases transfer of blood urea N to cecal microbial N in growing rabbits fed timothy hay (2011)
  • Author(s): Xiao Li
  • Organizer: 日本畜産学会第113回大会
  • Place of Presentation: 東京農業大学(演題受理後東日本大震災により中止)
  • Year and Date: 2011-03-29
• [Presentation] Fermentable sugar increases transfer of blood urea N to cecal microbial N in growing rabbits fed timothy hay (2011)
  • Author(s): Xiao Li,Sakaguchi Ei
  • Organizer: 日本畜産学会第113回大会
  • Place of Presentation: 東京農業大学(東京都)