Development of in vitro culture system for oocytes and embryos using marker assist selection together with pharmaceutical activation
| Project/Area Number |
22580316
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Applied animal science
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| Research Institution | Utsunomiya University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
FUKUI Emiko 宇都宮大学, 農学部, 准教授 (20208341)
YOSHIZAWA Midori 宇都宮大学, 農学部, 教授 (60114162)
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| Project Period (FY) |
2010 – 2012
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| Project Status |
Completed (Fiscal Year 2012)
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| Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2012: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2011: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2010: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Keywords | 繁殖 / 受胎率 / 着床率 / 遺伝子多型 / 胚発生 / 妊娠 / 胚発蛋 |
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| Research Abstract |
Nucleotide sequence of Bovine BRCA1 gene was highly conserved between Holstein cows and Japanese Black cows. Expression of Brca1 was strongly upregulated in implantation-competent blastocysts in the mouse, whereas the Brca1 expression of was low in blastocysts derived from in vitro fertilization. In contrast, 4-hydroxyestradiol and prolactin upregulated Brca1 levels in blastocysts derived from in vitro fertilization. Our results suggest that Brca1 most likely plays a physiological role indirecting implantation-competent blastocysts in the mouse.
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Report
(4 results)
Research Products
(62 results)
