Clarification of the mechanisms of action of prostaglandin and reactive oxygen species in bovine luteal endothelial cells
Project/Area Number |
22580318
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Single-year Grants |
Section | 一般 |
Research Field |
Applied animal science
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Research Institution | Okayama University |
Principal Investigator |
ACOSTA J, Tomas 岡山大学, 大学院・環境生命科学研究科, 准教授 (80379718)
|
Project Period (FY) |
2010 – 2012
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Project Status |
Completed (Fiscal Year 2012)
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Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2012: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2011: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2010: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
|
Keywords | ウシ / 繁殖 / 発情周期 / 黄体 / プロスタグランジン / 活性酸素 / 妊娠 / 効率的生産 / グルココルチコイド / 黄体細胞 / 一酸化窒素 / 黄体退行 / 内分泌 / プロスタグランジンF2α / 血管内皮細胞 |
Research Abstract |
Prostaglandin F2αand reactive oxygen species (ROS) regulates the corpus luteum (CL) function. The accumulation of ROS is controlled by superoxide dismutase (SOD). Thus, PGF may induce luteolysis by decreasing the expression of SOD and its bioactivity. We examined whether SOD is involved in the luteolytic action of PGF in bovine CL. SOD protein expression and SOD activity increased at 2 h but decreased at 24 h after administration of a luteolytic dose of PGF.In addition, PGF and H2O2increased SOD1 protein expression and SOD activity at 2 h but suppressed it at 24 h in cultured luteal cells. Furthermore, H2O2increased PGF production by luteal endothelial cells and steroidogenic cells in a dose- and time-dependent manner. PGF in turn, induced ROS production. These results indicate that PGF via interaction with ROS regulate bovineluteal SOD in a biphasic manner with an initial increase at time of functional luteolysis (2 h) followed by a decrease at the time of structural luteolysis (24 h). The down regulation of SOD during structural luteolysis may enhance ROS production and luteal cell death to ensure the regression of bovine CL.
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Report
(4 results)
Research Products
(64 results)
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[Journal Article] Expression of Glucocorticoid Receptor α and Its Regulation in the Bovine Endometrium: Possible Role in Cyclic Prostaglandin F2α Production2013
Author(s)
Kuse, M., Lee, HY., Acosta, TJ., Hojo, T., Okuda, K.
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Journal Title
Journal of Reproduction and Development
Volume: 59
Issue: 4
Pages: 346-352
DOI
NAID
ISSN
0916-8818, 1348-4400
Related Report
Peer Reviewed
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[Journal Article] Seasonal changes in luteal progesterone concentration and mRNA expressions of progesterone synthesis-related proteins in the corpus luteum of mares.2012
Author(s)
Kozai, K., Hojo, T., Takahashi, M., Acosta, TJ., Nambo, Y., Okuda, K
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Journal Title
J Reprod Dev
Volume: 58
Pages: 393-397
Related Report
Peer Reviewed
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[Journal Article] Conversion of cortisone to cortisol and prostaglandin F2αproduction by the reproductive tract of cows at the late luteal stage in vivo2012
Author(s)
Duong, H., Skarzynski, DJ., Piotrowska-Tomala, KK., Bah, M., Jankowska, P., Lukasik, K., Okuda, K., Acosta, TJ.
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Journal Title
Reprod Domest Anim
Volume: 47
Pages: 939-945
Related Report
Peer Reviewed
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[Journal Article] Effects of cortisol on pregnancy rate and corpus luteum function in heifers: an in vivo study2012
Author(s)
Duong, HT., Piotrowska-Tomala, KK., Acosta, TJ., Bah, MM., Sinderewicz, E., Majewska, M., Jankowska, K., Okuda, K., Skarzynski, DJ.
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Journal Title
J Reprod Dev
Volume: 58
Pages: 223-230
Related Report
Peer Reviewed
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[Journal Article] Acute changes in the concentrations of prostaglandin F2α(PGF) and cortisol in uterine and ovarian venous blood during PGF-induced luteolysis in cows2012
Author(s)
Duong, H., Vu, H, Bah M, Woclawek-Potocka, I., Dam, T., Skarzynski, D., Okuda, K., Acosta, TJ.
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Journal Title
Reprod Domest Anim
Volume: 47
Pages: 238-243
Related Report
Peer Reviewed
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[Journal Article] Is cortisol a modulator of interferon tau action in the endometrium during early pregnancy in cattle?2012
Author(s)
Majewska, M., Lee, HY., Tasaki Y., Acosta TJ., Szostek, AZ., Siemieniuch, M., Okuda, K., Skarzynski, DJ
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Journal Title
J Reprod Immunol
Volume: 93
Pages: 82-93
Related Report
Peer Reviewed
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