| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2012: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2011: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2010: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
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| Research Abstract |
Prostaglandin F2αand reactive oxygen species (ROS) regulates the corpus luteum (CL) function. The accumulation of ROS is controlled by superoxide dismutase (SOD). Thus, PGF may induce luteolysis by decreasing the expression of SOD and its bioactivity. We examined whether SOD is involved in the luteolytic action of PGF in bovine CL. SOD protein expression and SOD activity increased at 2 h but decreased at 24 h after administration of a luteolytic dose of PGF.In addition, PGF and H2O2increased SOD1 protein expression and SOD activity at 2 h but suppressed it at 24 h in cultured luteal cells. Furthermore, H2O2increased PGF production by luteal endothelial cells and steroidogenic cells in a dose- and time-dependent manner. PGF in turn, induced ROS production. These results indicate that PGF via interaction with ROS regulate bovineluteal SOD in a biphasic manner with an initial increase at time of functional luteolysis (2 h) followed by a decrease at the time of structural luteolysis (24 h). The down regulation of SOD during structural luteolysis may enhance ROS production and luteal cell death to ensure the regression of bovine CL.
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