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Improvement of somatic cell nuclear transfer techniques in miniature pigs using a noninvasive evaluation system for reprogramming status

Research Project

Project/Area Number 22580321
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Applied animal science
Research InstitutionKagoshima University

Principal Investigator

MIYOSHI Kazuchika  鹿児島大学, 農学部, 准教授 (70363611)

Co-Investigator(Kenkyū-buntansha) SATO Masahiro  鹿児島大学, 医用ミニブタ・先端医療開発 研究センター, 教授 (30287099)
YOSHIDA Mitsutoshi  鹿児島大学, 農学部, 教授 (00174954)
Project Period (FY) 2010 – 2012
Project Status Completed (Fiscal Year 2012)
Budget Amount *help
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2012: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2011: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2010: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Keywords体細胞クローニング / 核移植 / リプログラミング / ミニブタ
Research Abstract

It was indicated that treatment with some histone deacetylase inhibitors, such as valproic acid, suberoyl bishydroxamic acid and scriptaid, is beneficial for the efficient production of miniature pig somatic cell nuclear transfer embryos using a noninvasive evaluation system for the reprogramming status of donor nuclei on the basis of Oct-3/4 expression. In addition, an alive transgenic miniature pig expressing human apolipoprotein(a), a risk factor for the development of atherosclerosis, was successfully obtained using treatment with scriptaid.

Report

(4 results)
  • 2012 Annual Research Report   Final Research Report ( PDF )
  • 2011 Annual Research Report
  • 2010 Annual Research Report
  • Research Products

    (35 results)

All 2013 2012 2011 2010 Other

All Journal Article (17 results) (of which Peer Reviewed: 17 results) Presentation (13 results) Book (2 results) Remarks (2 results) Patent(Industrial Property Rights) (1 results)

  • [Journal Article] Effect of postactivation treatment with latrunculin A on in vitro and in vivo development of cloned embryos derived from kidney fibroblasts of an aged Clawn miniature boar2012

    • Author(s)
      Himaki T, Mizobe Y, Tsuda K, Suetomo M, Yamakuchi H, Miyoshi K, Takao S, Yoshida M
    • Journal Title

      Journal of Reproduction and Development

      Volume: 58 Pages: 398-403

    • NAID

      10031066746

    • Related Report
      2012 Annual Research Report 2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Gene expression differences in oocytes derived from adult and prepubertal Japanese Black Cattle during in vitro maturation2012

    • Author(s)
      Dorji
    • Journal Title

      Reproduction in Domestic Animals

      Volume: 47 Issue: 3 Pages: 392-402

    • DOI

      10.1111/j.1439-0531.2011.01887.x

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Determination of the Optimal Concentration of Several Selective Drugs Useful for Generating Multi-Transgenic Porcine Embryonic Fibroblasts2012

    • Author(s)
      Sato M, Ohtsuka M, Miura H, Miyoshi K, Watanabe S.
    • Journal Title

      Reproduction in Domestic Animals/John Wiley & Sons, Inc.

      Volume: 47 Issue: 5 Pages: 759-765

    • DOI

      10.1111/j.1439-0531.2011.01964.x

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Gene expression profile differences in embryos derived from prepubertal and adult Japanese Black Cattle during in vitro development2012

    • Author(s)
      Dorji
    • Journal Title

      Reproduction, Fertility and Development

      Volume: 24 Issue: 2 Pages: 370-381

    • DOI

      10.1071/rd11048

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Expression analysis of an α-1,3-galactosyltransferase, an enzyme that creates xenotransplantation-related α-Gal epitope, in pig preimplantation embryos2012

    • Author(s)
      Chi H
    • Journal Title

      Animal Science Journal

      Volume: 83 Issue: 1 Pages: 88-93

    • DOI

      10.1111/j.1740-0929.2011.00964.x

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Successful Suppression of Endogenous <i>&alpha;-1,3-Galactosyltransferase</i> Expression by RNA Interference in Pig Embryos Generated <i>In Vitro</i>2012

    • Author(s)
      Chi H
    • Journal Title

      Journal of Reproduction and Development

      Volume: 58 Issue: 1 Pages: 69-76

    • DOI

      10.1262/jrd.10-165M

    • NAID

      130001170088

    • ISSN
      0916-8818, 1348-4400
    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Whole-genome amplification-based GenomiPhi for multiple genomic analysis of individual early porcine embryos2011

    • Author(s)
      Akasaka E
    • Journal Title

      Theriogenology

      Volume: 75 Issue: 8 Pages: 1543-1549

    • DOI

      10.1016/j.theriogenology.2010.12.018

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Cultivation with untransfected fibroblasts stimulates proliferation of a single gene-modified fibroblast derived from a Clawn miniature swine fetus2011

    • Author(s)
      Sato M
    • Journal Title

      Reproduction in Domestic Animals

      Volume: 46 Issue: 5 Pages: 911-916

    • DOI

      10.1111/j.1439-0531.2010.01727.x

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Production of genetically modified porcine blastocysts by somatic cell nuclear transfer: Preliminary results toward production of xenograft-competent miniature pigs2010

    • Author(s)
      Himaki T, WatanabeS, ChiH, YoshidaM, Miyoshi K, Sato M
    • Journal Title

      Journal of Reproduction and Development

      Volume: 56 Pages: 630-638

    • NAID

      10027730311

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Effects of trichostatin A on in vitro development and transgene function in somatic cell nuclear transfer embryos derived from transgenic Clawn miniature pig cells2010

    • Author(s)
      Himaki T, Yokomine T, Sato M, Takao S, Miyoshi K, Yoshida M
    • Journal Title

      Animal Science Journal

      Volume: 81 Issue: 5 Pages: 558-563

    • DOI

      10.1111/j.1740-0929.2010.00772.x

    • NAID

      10027889588

    • Related Report
      2012 Final Research Report 2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Stage-specific effects of osmolarity of a culture medium on development of pig oocytes and miniature pig somatic cell nuclear transfer embryos activated by ultrasound treatment2010

    • Author(s)
      Mizobe Y, Kurino S, Sata Y, Mori H, Yoshida M, Miyoshi K
    • Journal Title

      Animal Science Journal

      Volume: 81 Pages: 453-460

    • NAID

      10027889195

    • Related Report
      2012 Final Research Report 2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Beneficial effects of reversine on in vitro development of miniature pig somatic cell nuclear transfer embryos2010

    • Author(s)
      Miyoshi K, Mori H, Mizobe Y, Himaki T, Yoshida M, Sato M
    • Journal Title

      Journal of Reproduction and Development

      Volume: 56 Pages: 291-296

    • NAID

      10026324772

    • Related Report
      2012 Final Research Report 2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Enhancement of cytoplasmic maturation of in vitro-matured pig oocytes by mechanical vibration2010

    • Author(s)
      Mizobe Y, Yoshida M, Miyoshi K
    • Journal Title

      Journal of Reproduction and Development

      Volume: 56 Pages: 285-290

    • NAID

      10026324747

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Latrunculin A dramatically improves the developmental capacity of nuclear tramsfer embryos derived from gene-modified Clawn miniature pig cells2010

    • Author(s)
      Himaki T, Mori H, Mizobe Y, Miyoshi K, Sato M, Takao S, Yoshida M
    • Journal Title

      Cellular Reprogramming

      Volume: 12 Issue: 2 Pages: 127-131

    • DOI

      10.1089/cell.2009.0066

    • Related Report
      2012 Final Research Report 2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Enhancement of cytoplasmic maturation of in vitromatured pig oocytes by mechanical vibration2010

    • Author(s)
      Mizobe Y
    • Journal Title

      Journal of Reproduction and Development

      Volume: 56 Pages: 281-290

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Usefulness of a non-invasive reporter system for monitoring reprogramming state in pig cells : Results of a cell fusion experiment2010

    • Author(s)
      Ozawa A
    • Journal Title

      Journal of Reproduction and Development

      Volume: 56 Pages: 363-369

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Production of genetically modified porcine blastocysts by somatic cell nuclear transfer : Preliminary results toward production of xenograft-competent miniature pigs2010

    • Author(s)
      Himaki T
    • Journal Title

      Journal of Reproduction and Development

      Volume: 56 Pages: 630-638

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Presentation] 成熟培養中の種々の期間における振動がブタ卵丘卵子複合体の膨化および卵子の単為発生に及ぼす影響2013

    • Author(s)
      長尾洋三
    • Organizer
      第116回日本畜産学会
    • Place of Presentation
      安田女子大学
    • Year and Date
      2013-03-30
    • Related Report
      2012 Annual Research Report 2012 Final Research Report
  • [Presentation] 可溶型ヒトネクチン-1 発現クラウン系ミニブタクローン作出に関する研究.2013

    • Author(s)
      岩田一紘
    • Organizer
      第116回日本畜産学会
    • Place of Presentation
      安田女子大学
    • Year and Date
      2013-03-30
    • Related Report
      2012 Final Research Report
  • [Presentation] 電気活性化後のオキサムフラチン添加が遺伝子改変ミニブタ体細胞クローン胚の体外発生に及ぼす影響2013

    • Author(s)
      日巻武裕
    • Organizer
      第116回日本畜産学会
    • Place of Presentation
      安田女子大学
    • Year and Date
      2013-03-30
    • Related Report
      2012 Annual Research Report 2012 Final Research Report
  • [Presentation] 成熟および発生培養中の振動が電気刺激により活性化したブタ卵子の単為発生に及ぼす影響2013

    • Author(s)
      児島輝仁
    • Organizer
      第115回日本畜産学会
    • Place of Presentation
      名古屋大学
    • Year and Date
      2013-03-28
    • Related Report
      2012 Final Research Report
  • [Presentation] 成熟および発生培養中の振動が電気刺激により活性化したブタ卵子の単為発生に及ぼす影響2012

    • Author(s)
      児島輝仁
    • Organizer
      日本畜産学会第115回大会
    • Place of Presentation
      名古屋大学IB電子情報館(愛知県)
    • Year and Date
      2012-03-28
    • Related Report
      2011 Annual Research Report
  • [Presentation] ミニブタ体細胞クローン胚作出技術の高度化. 先進医用ブタの開発と前臨床研究拠点形成プロジェクト2012

    • Author(s)
      三好和睦
    • Organizer
      第2回公開シンポジウム~ブタの医用動物への展開~
    • Place of Presentation
      鹿児島大学
    • Year and Date
      2012-03-22
    • Related Report
      2012 Final Research Report
  • [Presentation] ミニブタ体細胞クローン胚作出技術の高度化2012

    • Author(s)
      三好和睦
    • Organizer
      先進医用ブタの開発と前臨床研究拠点形成プロジェクト第2回公開シンポジウム~ブタの医用動物への展開~
    • Place of Presentation
      鹿児島大学稲盛会館(鹿児島県)(招待講演)
    • Year and Date
      2012-03-22
    • Related Report
      2011 Annual Research Report
  • [Presentation] マイクロアレイを用いた黒毛和種ウシ体外成熟卵の網羅的遺伝子発現解析2011

    • Author(s)
      Dorji
    • Organizer
      第104回日本繁殖生物学会
    • Place of Presentation
      いわて県民情報交流センター・アイーナ(岩手県)
    • Year and Date
      2011-09-17
    • Related Report
      2011 Annual Research Report
  • [Presentation] 活性化後のスクリプタイドまたはスベロイルビスヒドロキサム酸処理がミニブタ体細胞クローン胚の体外発生に及ぼす影響2011

    • Author(s)
      小川純輝
    • Organizer
      第104回日本繁殖生物学会
    • Place of Presentation
      いわて県民情報交流センター・アイーナ
    • Year and Date
      2011-09-15
    • Related Report
      2012 Final Research Report
  • [Presentation] 異種移植関連抗原α-Galエピトープ陰性ブタ体細胞の選択的濃縮のためのtargeted toxinの有効性2011

    • Author(s)
      池海英
    • Organizer
      第104回日本繁殖生物学会
    • Place of Presentation
      いわて県民情報交流センター・アイーナ(岩手県)
    • Year and Date
      2011-09-15
    • Related Report
      2011 Annual Research Report
  • [Presentation] 活性化後のスクリプタイドまたはスベロイルビスヒドロキサム酸処理がミニブタ体細胞クローン胚の体外発生に及ぼす影響2011

    • Author(s)
      小川純輝
    • Organizer
      第104回日本繁殖生物学会
    • Place of Presentation
      いわて県民情報交流センター・アイーナ(岩手県)
    • Year and Date
      2011-09-15
    • Related Report
      2011 Annual Research Report
  • [Presentation] 全ゲノム増幅法による核移植ブタ胚でのgene-targeted allele検出の有効性2011

    • Author(s)
      池海英
    • Organizer
      第58回日本実験動物学会
    • Place of Presentation
      タワーホール船掘(東京都)
    • Year and Date
      2011-05-25
    • Related Report
      2011 Annual Research Report
  • [Presentation] 可溶型ヒトネクチン-1発現クラウン系ミニブタクローン作出に関する研究

    • Author(s)
      岩田一紘
    • Organizer
      第116回日本畜産学会
    • Place of Presentation
      安田女子大学
    • Related Report
      2012 Annual Research Report
  • [Book] Targeted toxin as a useful reagent for enrichment of x-Gal epitope-negative cells used for somatic cell nuclear transfer in pigs2012

    • Author(s)
      Sato M, Chi H, Miyoshi K. In Tech
    • Publisher
      Xenotransplantation
    • Related Report
      2012 Final Research Report
  • [Book] Xenotransplantation2012

    • Author(s)
      Sato M
    • Publisher
      In Tech
    • Related Report
      2011 Annual Research Report
  • [Remarks]

    • URL

      http://lms.agri.kagoshima-u.ac.jp/~animal/reproduction/index.html

    • Related Report
      2012 Final Research Report
  • [Remarks] 鹿児島大学農学部家畜生産学講座家畜繁殖学研究室ホームページ

    • URL

      http://lms.agri.kagoshima-u.ac.jp/~animal/reproduction/index.html

    • Related Report
      2012 Annual Research Report
  • [Patent(Industrial Property Rights)] 超音波による卵子またはクローン胚の活性化方法ならびに該方法により活性化したクローン胚からのクローン動物の作出2011

    • Inventor(s)
      三好和睦, 吉田光敏
    • Industrial Property Rights Holder
      鹿児島大学
    • Filing Date
      2011-07-08
    • Related Report
      2012 Final Research Report

URL: 

Published: 2010-08-23   Modified: 2019-07-29  

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