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Production and analysis of oocyte-like cells derived from embryonic stem cells.

Research Project

Project/Area Number 22580323
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Applied animal science
Research InstitutionKinki University

Principal Investigator

HOSOI Yoshihiko  近畿大学, 生物理工学部, 教授 (70192739)

Project Period (FY) 2010 – 2012
Project Status Completed (Fiscal Year 2012)
Budget Amount *help
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2012: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2011: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2010: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Keywords胚性幹細胞 / 卵子様細胞 / 始源生殖細胞 / 生殖細胞分化 / mES細胞 / 分化誘導法 / PGC様細胞 / 配偶子形成 / マウス胚性幹細胞 / 生殖細胞関連遺伝子 / 接着培養 / 生殖細胞系譜 / 三次元培養
Research Abstract

The difference in the stock line of embryonic stem cells or an incubation atmosphere can be considered as a factor which influences an efficiency of a differentiation induction from an embryonic stem cell line. In our results, it was suggested that a status of embryonic stem cells for differentiation to primordial germ cells in vitro was also important, not only other secreted substances.

Report

(4 results)
  • 2012 Annual Research Report   Final Research Report ( PDF )
  • 2011 Annual Research Report
  • 2010 Annual Research Report
  • Research Products

    (21 results)

All 2013 2012 2011 2010

All Journal Article (11 results) (of which Peer Reviewed: 9 results) Presentation (7 results) Book (3 results)

  • [Journal Article] Generation of embryonic stem cell lines from immature rabbit ovarian follicles.2013

    • Author(s)
      Teramura T, Sugimoto H, Frampton J, Kida Y, Nakano M, Kawakami M, Izumi H, Fukunaga N, Onodera Y, Takehara T, Fukuda K, Hosoi Y.
    • Journal Title

      Stem Cells Dev.

      Volume: 15;22(6) Pages: 928-38

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Generation of embryonic stem cell lines from immature rabbit ovarian follicles.2013

    • Author(s)
      Teramura, T., Hosoi, Y., et al.,
    • Journal Title

      Stem Cells Dev.

      Volume: 22 Pages: 928-938

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Embryonic Stem Cell: New Resource for Germ Cell Study.2012

    • Author(s)
      Fukunaga N, Kawakami M, Saeki K, Matsumoto K, Hosoi Y
    • Journal Title

      JMOR

      Volume: Vo.29, No.1

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Embryonic Stem Cell: New Resource for Germ Cell Study.2012

    • Author(s)
      Fukunaga, N., Teramura, T. & Hosoi Y.
    • Journal Title

      Jornal of Mammalian Ova Research

      Volume: 29 Pages: 11-16

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Development of interspecies cloned embryos reconstructed with rabbit (Oryctolagus cuniculus) oocytes and cynomolgus monkey (Macaca fascicularis) fibroblast cell nuclei.2012

    • Author(s)
      Yamochi, T., Hosoi,Y., et al.,
    • Journal Title

      Zygote

      Volume: April 5 Issue: 4 Pages: 1-9

    • DOI

      10.1017/s0967199412000019

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Embryonic Stem Cells : Novel Attractive Research Tool tor Germ Cell Development2012

    • Author(s)
      N.Fukunaga, T.Teramura, Y.Hosoi
    • Journal Title

      J.Mammalian Ova Research

      Volume: Vo.29,No.1(in print)

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] 再生医療からみた生殖医療の将来展望2011

    • Author(s)
      福永直人、細井美彦
    • Journal Title

      産婦人科の実際

      Volume: 60(5) Pages: 751-758

    • Related Report
      2012 Final Research Report 2011 Annual Research Report
  • [Journal Article] マウスエピブラスト幹細胞からの生殖細胞の分化誘導2011

    • Author(s)
      福永直人、荒田隆志、大野真奈、岸上哲士、細井美彦
    • Journal Title

      近畿大学先端技術総合研究所紀要

      Volume: 第16号 Pages: 19-26

    • NAID

      120005736480

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] 目で見る生殖と再生-体細胞クローンー2011

    • Author(s)
      矢持隆之、岸上哲士、細井美彦
    • Journal Title

      Hormone Frontier in Gynecology

      Volume: Vol.18 No.1 Pages: 2-5

    • Related Report
      2012 Final Research Report
  • [Journal Article] マウスエピブラスト幹細胞からの生殖細胞の分化誘導2011

    • Author(s)
      福永直人
    • Journal Title

      近畿大学先端技術総合研究所紀要

      Volume: vol.16(In print)

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Leukemia inhibitory factor (LIF) enhances germ cell differentiation from primate embryonic stem cells.2010

    • Author(s)
      Fukunaga N, Teramura, T, Onodera Y, Takehara T, Fukuda K, Hosoi Y.
    • Journal Title

      Cell Reprogram

      Volume: 12(4) Pages: 369-76

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Presentation] Derivation of primordial germ cells from mouse embryonic stem cells in a ground state pluripotency2012

    • Author(s)
      Fukunaga N. Takeshi Teramura, Toshiyuki Takehara, Yuta Onodera, Kanji Fukuda, Yoshihiko Hosoi
    • Organizer
      4th Congress of the ASPIRE (Asia Pacific Initiative on Reproduction)
    • Place of Presentation
      Osaka Iterntional Convention Center
    • Related Report
      2012 Final Research Report
  • [Presentation] 基底状態にあるマウスES 細胞からの始原生殖細胞の分化誘導2012

    • Author(s)
      福永直人、寺村岳士、竹原俊幸、小野寺勇太、福田寛二、細井美彦
    • Organizer
      第11回 日本再医療学会総会
    • Place of Presentation
      パシフィコ横浜
    • Related Report
      2012 Final Research Report
  • [Presentation] 基底状態にあるマウスES 細胞からの始原生殖細胞の分化誘導2012

    • Author(s)
      福永直人、細井美彦 他
    • Organizer
      第11回 日本再医療学会総会
    • Place of Presentation
      パシフィコ横浜
    • Related Report
      2012 Annual Research Report
  • [Presentation] ウサギ体外培養卵胞内卵子を用いた胚性幹細胞樹立2012

    • Author(s)
      川上真貴子、細井美彦 他
    • Organizer
      第30回 日本受精着床学会
    • Place of Presentation
      大阪国際会議場(グランキューブ大阪)
    • Related Report
      2012 Annual Research Report
  • [Presentation] 生殖細胞の分化誘導に適したマウス ES細胞の培養環境の検討2011

    • Author(s)
      福永直人,川上真貴子、矢持隆之、木田雄大、岸上哲士、細井美彦
    • Organizer
      第29回受精着床学会
    • Place of Presentation
      京王プラザホテル
    • Related Report
      2012 Final Research Report
  • [Presentation] 生殖細胞の分化誘導に適したマウスES細胞の培養環境の検討2011

    • Author(s)
      福永、細井, 他
    • Organizer
      第29回受精着床学会
    • Place of Presentation
      京王プラザホテル(東京都)
    • Related Report
      2011 Annual Research Report
  • [Presentation] 良好胚の作製2010

    • Author(s)
      細井美彦
    • Organizer
      第55回日本生殖医学会総会・学術講演会
    • Place of Presentation
      徳島県郷土文化会館(徳島市)
    • Year and Date
      2010-11-12
    • Related Report
      2010 Annual Research Report
  • [Book] 生殖卵巣学臨床への進展、卵子の発生2011

    • Author(s)
      細井美彦、福永直人
    • Related Report
      2012 Final Research Report
  • [Book] 生殖卵巣学臨床への進展,第1章,2-1卵子の発生(分担執筆)2011

    • Author(s)
      細井美彦、福永直人
    • Total Pages
      296
    • Publisher
      医歯薬出版株式会社
    • Related Report
      2011 Annual Research Report
  • [Book] 生殖卵巣学第一章2節(1)卵子の発生2011

    • Author(s)
      細井美彦
    • Total Pages
      191
    • Publisher
      医歯薬出版株式会社
    • Related Report
      2010 Annual Research Report

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Published: 2010-08-23   Modified: 2019-07-29  

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