Production and analysis of oocyte-like cells derived from embryonic stem cells.

• Project/Area Number: 22580323
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Applied animal science
• Research Institution: Kinki University
• Principal Investigator: HOSOI Yoshihiko 近畿大学, 生物理工学部, 教授 (70192739)
• Project Period (FY): 2010 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000); Fiscal Year 2012: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2011: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2010: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
• Keywords: 胚性幹細胞 / 卵子様細胞 / 始源生殖細胞 / 生殖細胞分化 / mES細胞 / 分化誘導法 / PGC様細胞 / 配偶子形成 / マウス胚性幹細胞 / 生殖細胞関連遺伝子 / 接着培養 / 生殖細胞系譜 / 三次元培養

Research Abstract

The difference in the stock line of embryonic stem cells or an incubation atmosphere can be considered as a factor which influences an efficiency of a differentiation induction from an embryonic stem cell line. In our results, it was suggested that a status of embryonic stem cells for differentiation to primordial germ cells in vitro was also important, not only other secreted substances.

Research Products

• [Journal Article] Generation of embryonic stem cell lines from immature rabbit ovarian follicles. (2013)
  • Author(s): Teramura T, Sugimoto H, Frampton J, Kida Y, Nakano M, Kawakami M, Izumi H, Fukunaga N, Onodera Y, Takehara T, Fukuda K, Hosoi Y.
  • Journal Title: Stem Cells Dev. Volume: 15;22(6) Pages: 928-38
  • Peer Reviewed
• [Journal Article] Generation of embryonic stem cell lines from immature rabbit ovarian follicles. (2013)
  • Author(s): Teramura, T., Hosoi, Y., et al.,
  • Journal Title: Stem Cells Dev. Volume: 22 Pages: 928-938
  • Peer Reviewed
• [Journal Article] Embryonic Stem Cell: New Resource for Germ Cell Study. (2012)
  • Author(s): Fukunaga N, Kawakami M, Saeki K, Matsumoto K, Hosoi Y
  • Journal Title: JMOR Volume: Vo.29, No.1
  • Peer Reviewed
• [Journal Article] Embryonic Stem Cell: New Resource for Germ Cell Study. (2012)
  • Author(s): Fukunaga, N., Teramura, T. & Hosoi Y.
  • Journal Title: Jornal of Mammalian Ova Research Volume: 29 Pages: 11-16
  • Peer Reviewed
• [Journal Article] Development of interspecies cloned embryos reconstructed with rabbit (Oryctolagus cuniculus) oocytes and cynomolgus monkey (Macaca fascicularis) fibroblast cell nuclei. (2012)
  • Author(s): Yamochi, T., Hosoi,Y., et al.,
  • Journal Title: Zygote Volume: April 5 Issue: 4 Pages: 1-9
  • DOI: 10.1017/s0967199412000019
  • Peer Reviewed
• [Journal Article] Embryonic Stem Cells : Novel Attractive Research Tool tor Germ Cell Development (2012)
  • Author(s): N.Fukunaga, T.Teramura, Y.Hosoi
  • Journal Title: J.Mammalian Ova Research Volume: Vo.29,No.1(in print)
  • Peer Reviewed
• [Journal Article] 再生医療からみた生殖医療の将来展望 (2011)
  • Author(s): 福永直人、細井美彦
  • Journal Title: 産婦人科の実際 Volume: 60(5) Pages: 751-758
• [Journal Article] マウスエピブラスト幹細胞からの生殖細胞の分化誘導 (2011)
  • Author(s): 福永直人、荒田隆志、大野真奈、岸上哲士、細井美彦
  • Journal Title: 近畿大学先端技術総合研究所紀要 Volume: 第16号 Pages: 19-26
  • NAID: 120005736480
  • Peer Reviewed
• [Journal Article] 目で見る生殖と再生-体細胞クローンー (2011)
  • Author(s): 矢持隆之、岸上哲士、細井美彦
  • Journal Title: Hormone Frontier in Gynecology Volume: Vol.18 No.1 Pages: 2-5
• [Journal Article] マウスエピブラスト幹細胞からの生殖細胞の分化誘導 (2011)
  • Author(s): 福永直人
  • Journal Title: 近畿大学先端技術総合研究所紀要 Volume: vol.16(In print)
  • Peer Reviewed
• [Journal Article] Leukemia inhibitory factor (LIF) enhances germ cell differentiation from primate embryonic stem cells. (2010)
  • Author(s): Fukunaga N, Teramura, T, Onodera Y, Takehara T, Fukuda K, Hosoi Y.
  • Journal Title: Cell Reprogram Volume: 12(4) Pages: 369-76
  • Peer Reviewed
• [Presentation] Derivation of primordial germ cells from mouse embryonic stem cells in a ground state pluripotency (2012)
  • Author(s): Fukunaga N. Takeshi Teramura, Toshiyuki Takehara, Yuta Onodera, Kanji Fukuda, Yoshihiko Hosoi
  • Organizer: 4th Congress of the ASPIRE (Asia Pacific Initiative on Reproduction)
  • Place of Presentation: Osaka Iterntional Convention Center
• [Presentation] 基底状態にあるマウスES 細胞からの始原生殖細胞の分化誘導 (2012)
  • Author(s): 福永直人、寺村岳士、竹原俊幸、小野寺勇太、福田寛二、細井美彦
  • Organizer: 第11回 日本再医療学会総会
  • Place of Presentation: パシフィコ横浜
• [Presentation] 基底状態にあるマウスES 細胞からの始原生殖細胞の分化誘導 (2012)
  • Author(s): 福永直人、細井美彦 他
  • Organizer: 第11回 日本再医療学会総会
  • Place of Presentation: パシフィコ横浜
• [Presentation] ウサギ体外培養卵胞内卵子を用いた胚性幹細胞樹立 (2012)
  • Author(s): 川上真貴子、細井美彦 他
  • Organizer: 第30回 日本受精着床学会
  • Place of Presentation: 大阪国際会議場(グランキューブ大阪)
• [Presentation] 生殖細胞の分化誘導に適したマウス ES細胞の培養環境の検討 (2011)
  • Author(s): 福永直人,川上真貴子、矢持隆之、木田雄大、岸上哲士、細井美彦
  • Organizer: 第29回受精着床学会
  • Place of Presentation: 京王プラザホテル
• [Presentation] 生殖細胞の分化誘導に適したマウスES細胞の培養環境の検討 (2011)
  • Author(s): 福永、細井, 他
  • Organizer: 第29回受精着床学会
  • Place of Presentation: 京王プラザホテル(東京都)
• [Presentation] 良好胚の作製 (2010)
  • Author(s): 細井美彦
  • Organizer: 第55回日本生殖医学会総会・学術講演会
  • Place of Presentation: 徳島県郷土文化会館(徳島市)
  • Year and Date: 2010-11-12
• [Book] 生殖卵巣学臨床への進展、卵子の発生 (2011)
  • Author(s): 細井美彦、福永直人
• [Book] 生殖卵巣学臨床への進展,第1章,2-1卵子の発生(分担執筆) (2011)
  • Author(s): 細井美彦、福永直人
  • Total Pages: 296
  • Publisher: 医歯薬出版株式会社
• [Book] 生殖卵巣学第一章2節(1)卵子の発生 (2011)
  • Author(s): 細井美彦
  • Total Pages: 191
  • Publisher: 医歯薬出版株式会社