Improvement of in vitro production system for porcine embryos by elucidation of mechanism for oocyte maturation in a chemically defined medium
| Project/Area Number |
22580324
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Applied animal science
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| Research Institution | National Agriculture and Food Research Organization |
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Principal Investigator |
YOSHIOKA Koji 独立行政法人農業・食品産業技術総合研究機構, 動物衛生研究所・病態研究領域, 上席研究員 (20355192)
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| Co-Investigator(Renkei-kenkyūsha) |
HOSHI Hiroyoshi 株式会社機能性ペプチド研究所, 取締役所長 (50500708)
MITO Tomomi 株式会社機能性ペプチド研究所, 研究員
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| Project Period (FY) |
2010 – 2012
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| Project Status |
Completed (Fiscal Year 2012)
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| Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2012: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2011: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2010: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Keywords | 発生・分化 / 卵子成熟 / 応用動物 / ブタ / 体外生産 / 卵丘膨化 / TGF-α / FSH / HAPLN |
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| Research Abstract |
The addition of follicle-stimulating hormone (FSH) and tansforming growth factor (TGF)-α to the defined IVM medium synergistically enhanced nuclear maturation, cumulus expansion and the developmental ability of porcine embryos. FSH and TGF-α appear to enhance porcine oocyte maturation through cumulus cells by partly different modes of action. Exogenous hyaluronan and proteoglycan binding link protein 1 and versican also stimulated cumulus expansion.
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Report
(4 results)
Research Products
(12 results)
