Improvement of in vitro production system for porcine embryos by elucidation of mechanism for oocyte maturation in a chemically defined medium

• Project/Area Number: 22580324
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Applied animal science
• Research Institution: National Agriculture and Food Research Organization
• Principal Investigator: YOSHIOKA Koji 独立行政法人農業・食品産業技術総合研究機構, 動物衛生研究所・病態研究領域, 上席研究員 (20355192)
• Co-Investigator(Renkei-kenkyūsha): HOSHI Hiroyoshi 株式会社機能性ペプチド研究所, 取締役所長 (50500708) ; MITO Tomomi 株式会社機能性ペプチド研究所, 研究員
• Project Period (FY): 2010 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000); Fiscal Year 2012: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2011: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2010: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
• Keywords: 発生・分化 / 卵子成熟 / 応用動物 / ブタ / 体外生産 / 卵丘膨化 / TGF-α / FSH / HAPLN

Research Abstract

The addition of follicle-stimulating hormone (FSH) and tansforming growth factor (TGF)-α to the defined IVM medium synergistically enhanced nuclear maturation, cumulus expansion and the developmental ability of porcine embryos. FSH and TGF-α appear to enhance porcine oocyte maturation through cumulus cells by partly different modes of action. Exogenous hyaluronan and proteoglycan binding link protein 1 and versican also stimulated cumulus expansion.

Research Products

• [Journal Article] Recombinant human follicle- stimulating hormone and transforming growth factor-α enhance in vitro maturation of porcine oocytes (2013)
  • Author(s): Mito T, Yoshioka K, Noguchi M, Yamashita S, Hoshi H.
  • Journal Title: Mol. Reprod Volume: 80 Issue: 7 Pages: 549-560
  • DOI: 10.1002/mrd.22190
  • Peer Reviewed
• [Journal Article] Recombinant human follicle-stimulating hormone and transforming growth factor-α enhance in vitro maturation of porcine oocytes (2013)
  • Author(s): Mito T, Yoshioka K, Noguchi M, Yamashita S, Hoshi H
  • Journal Title: Molecular Reproduction and Development Volume: 80
  • Peer Reviewed
• [Journal Article] Glucose and glycine synergistically enhance in vitro development of porcine blastocysts in a chemically defined medium (2012)
  • Author(s): Mito T, Yoshioka K, Yamashita S, Suzuki C, Noguchi M, Hoshi H
  • Journal Title: Reproduction, Fertility and Development Volume: VOL.24 Issue: 3 Pages: 443-450
  • DOI: 10.1071/rd11197
  • Peer Reviewed
• [Journal Article] Production of piglets from in vitro-produced embryos following non-surgical transfer (2012)
  • Author(s): Yoshioka K, Noguchi M, Suzuki C
  • Journal Title: Animal Reproduction Science Volume: VOL.131 Issue: 1-2 Pages: 23-29
  • DOI: 10.1016/j.anireprosci.2012.01.018
  • Peer Reviewed
• [Journal Article] Development and Application of a Chemically Defined Medium for the In Vitro Production of Porcine Embryos (2011)
  • Author(s): Yoshioka K.
  • Journal Title: Journal of Reproduction and Development Volume: 57 Issue: 1 Pages: 9-16
  • DOI: 10.1262/jrd.10-196E
  • NAID: 10027900771
  • ISSN: 0916-8818, 1348-4400
  • Peer Reviewed
• [Journal Article] Development and application of a chemically defined medium for the in vitro production of porcine embryos (2011)
  • Author(s): Yoshioka K
  • Journal Title: Journal of Reproduction and Development Volume: 57 Pages: 9-16
  • NAID: 10027900771
  • Peer Reviewed
• [Presentation] Hyaluronan and proteoglycan binding link protein 1 and versican enhance cumulus expansion during in vitro maturation of porcine cumulus oocyte complexes (2012)
  • Author(s): Yoshioka K, Noguchi M, Mito T
  • Organizer: The 17th International Congress on Animal Reproduction
  • Place of Presentation: Vancouver, Canada
  • Year and Date: 2012-07-31
• [Presentation] Hyaluronan and proteoglycan binding link protein 1 and versican enhance cumulus expansion during in vitro maturation of porcine cumulus oocyte complexes (2012)
  • Author(s): Yoshioka K, Noguchi M, Mito T
  • Organizer: The 17th International Congress on Animal Reproduction
  • Place of Presentation: バンクーバー（カナダ）
• [Presentation] 化学組成の明らかな培地によるブタ胚の体外生産に関する研究 (2010)
  • Author(s): 吉岡耕治
  • Organizer: 第103回日本繁殖生物学会大会
  • Place of Presentation: 十和田市
  • Year and Date: 2010-09-03
• [Presentation] ブタ卵子の成熟におけるリコンビナントヒト卵胞刺激ホルモン(rhFSH)およびTGF-αの影響と作用機構の解明 (2010)
  • Author(s): 水戸友美、吉岡耕治、鈴木千恵、山下祥子、星宏良
  • Organizer: 第103回日本繁殖生物学会大会
  • Place of Presentation: 十和田市
  • Year and Date: 2010-09-02
• [Presentation] ブタ卵子の成熟におけるリコンビナントヒト卵胞刺激ホルモン(rhFSH)およびTGF-αの影響と作用機構の解明 (2010)
  • Author(s): 水戸友美、吉岡耕治、鈴木千恵、山下祥子、星宏良
  • Organizer: 日本繁殖生物学会
  • Place of Presentation: 北里大学(青森県)
  • Year and Date: 2010-09-02
• [Presentation] ブタ体外生産胚の移植による子ブタ生産の現状と展望
  • Author(s): 吉岡 耕治
  • Organizer: NIAS/NILGS合同シンポジウム「動物生殖技術研究の現状と今後の展望」
  • Place of Presentation: つくば国際会議場（茨城県）
  • Invited