| Project/Area Number |
22580325
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
|
| Allocation Type | Single-year Grants |
|---|
| Section | 一般 |
|---|
| Research Field |
Applied animal science
|
|---|
| Research Institution | National Agriculture and Food Research Organization |
|---|
Principal Investigator |
TAGAMI Takahiro 独立行政法人農業・食品産業技術総合研究機構, 畜産草地研究所・家畜育種繁殖研 究領域, 主任研究員 (60355104)
|
|---|
| Co-Investigator(Renkei-kenkyūsha) |
OGAWA Haruko 帯広畜産大学, 動物・食品衛生研究センター, 准教授 (10400079)
|
|---|
| Research Collaborator |
OISHI Isao 独立行政法人産業総合技術研究所, 健康工学研究部門, 主任研究員 (50314472)
KAGAMI Hiroshi 信州大学, 農学部・食料生産科学科, 教授 (80308303)
|
|---|
| Project Period (FY) |
2010 – 2012
|
| Project Status |
Completed (Fiscal Year 2012)
|
| Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2012: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2011: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2010: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
|
|---|
| Keywords | ニワトリ / 始原生殖細胞 / トランスジェニックニワトリ / Vasa ホモログタンパク質(CVH) / レンチウイルスベクター / バイオテクノロジー / vasa 遺伝子 / トランスジェニック / ニワトリVasaホモログタンパク質(Cvh) / 緑色蛍光タンパク(GFP) |
|---|
| Research Abstract |
Visualization of chicken germ cells is useful to generate for transgenic chickens and critical to understanding the differentiation and proliferationof germ cells. As a first step, chicken vasa homologue (CVH) promoter sequence was searched. We tried to produce the transgenic chicken line in which the germline cells were visualized by CVH promoter-driven YFP or GFP expression. However, no germ cells in gonads of thetransgenic chickens expressed the fluorescence signal.
|
