Transcriptomic profiling of skeletal muscle microRNA involved in muscle fiber type specification
| Project/Area Number |
22580326
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
|
| Allocation Type | Single-year Grants |
|---|
| Section | 一般 |
|---|
| Research Field |
Applied animal science
|
|---|
| Research Institution | National Agriculture and Food Research Organization |
|---|
Principal Investigator |
MUROYA Susumu 独立行政法人農業・食品産業技術総合研究機構, 畜産草地研究所畜産物研究領域, 上席研究員 (50355062)
|
|---|
| Project Period (FY) |
2010 – 2012
|
| Project Status |
Completed (Fiscal Year 2012)
|
| Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2012: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2011: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2010: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
|
|---|
| Keywords | ウシ / 筋線維型 / 骨格筋 / マイクロRNA / 次世代シーケンサー |
|---|
| Research Abstract |
To elucidate roles of miRNA in bovine muscle type specification and maintenance, we sought to determine differentially expressed miRNA between semitendinosus (STD) and masseter (MS) muscles from 3 Japanese black cattle by massively parallel sequencing. Differential gene expression of myosin heavy chain (MyHC) isoforms confirmed that STD and MS were MyHC-2x- and MyHC-1-abundant muscles, respectively. In total, 192 known miRNA and 20 potential new bovine miRNA were obtained from the sequencing. The differentially expressed miRNA with more than 2-fold difference in each muscle were identified. In particular, miR-196a and miR-885 were exclusively expressed in STD muscle, which was validated by quantitative reverse transcription-PCR (P=0.045 and P<0.001, respectively), whereas a slow type-directing miR-208b was highly expressed in MS compared with STD (false discovery rate<0.05). In addition, 16 potential novel miRNA were mapped and confirmed for their precursor structures by computational analyses.
|
Report
(4 results)
Research Products
(17 results)
-
-
-
-
-
-
-
-
-
-
-
-
-
[Presentation] Structural functions of skeletal muscle-specific calpain-3 in Ca2+ efflux from the sarcoplasmic reticulum2011
Author(s)
尾嶋孝一, Ono, Y., Ottenheijm, C., Hata, S., Suzuki, H., 大江美香, 中島郁世, 室谷進, Granzier, H., Sorimachi, H.
Organizer
2011 Annual Meeting of the American Society for Cell Biology
Place of Presentation
コロラド州デンバー(アメリカ)
Related Report
-
-
-
-
