Biological activities of ovine and bovine placental prolactin family proteins

• Project/Area Number: 22580327
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Applied animal science
• Research Institution: National Institute of Agrobiological Sciences
• Principal Investigator: TAKAHASHI Toru 独立行政法人農業生物資源研究所, 動物生産生理機能研究ユニット, 上級研究員 (20355738)
• Project Period (FY): 2010 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2012: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2011: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2010: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
• Keywords: ウシ / 胎盤 / プロラクチン / カテプシン / 血管内皮細胞 / MMP / 7S / IV型コラーゲン

Research Abstract

Bovine prolactin-related protein-I (PRP1) is a non-classical member of prolactin (PRL) family proteins. However, its function is still unknown. PRL, when cleaved by cathepsin D and matrix metalloproteinases (MMPs), resulted in N-terminal 16 kDa fragments (16K-PRL) that have antiangiogenetic properties in human and rodents. We examined the possibility of similar activity of bovine PRP1. PRP1 (normally 33 kDa) was cleaved by cathepsins (CTSs), MMPs, and bovine cotyledonary-conditioned medium (BCCM), and generated mainly 26 kDa N-terminal fragments. Two specific enzyme families, CTSs and MMPs cleaved intact PRP1, and BCCM also contained PRP1 cleavage activity. Bioactivity of cleaved PRP1 was examined in a cell proliferation assay using bovine brain vascular endothelial cells. The cleaved PRP1 stimulated the proliferation of endothelial cells in vitro. The endothelial cell proliferation activity of cleaved PRP1 may be shared in specific bovine placentomal angiogenesis.

Research Products

• [Journal Article] Biology of the placental proteins in domestic ruminants: Expression, proposed roles and practical applications (2013)
  • Author(s): Takahashi T, Hosoe M and Hayashi KG
  • Journal Title: Japan Agricultural Research Quarterly Volume: 47 Pages: 43-51
  • NAID: 130003377757
  • Peer Reviewed
• [Journal Article] Expression of extracellular matrix metalloproteinase inducer (EMMPRIN) and its expected roles in the bovine endometrium during gestation. (2011)
  • Author(s): Mishra B, Kizaki K, Koshi K, Ushizawa K, Takahashi T, Hosoe M, Sato T, Ito A, Hashizume K
  • Journal Title: Domest Anim Endocrinol Volume: 42 Pages: 63-73
  • Peer Reviewed
• [Journal Article] 着床とプロラクチン-ウシPRL/GHファミリーは着床誘導物質か? (2011)
  • Author(s): 橋爪一善 、高橋 透、木崎景一郎
  • Journal Title: HORMONE FRONTIER IN GYNECOLOGY Volume: 18 Pages: 259-267
  • Peer Reviewed
• [Journal Article] Expression of extracellular matrix metalloproteinase inducer (EMMPRIN) and its expected roles in the bovine endometrium during gestation (2011)
  • Author(s): Mishra B, Kizaki K, Koshi K, Ushizawa K, Takahashi T, Hosoe M, Sato T, Ito A, Hashizume K
  • Journal Title: Domest Anim Endocrinol Volume: 42 Pages: 63-73
  • Peer Reviewed
• [Journal Article] 着床とプロラクチンーウシPRL/GHファミリーは着床誘導物質か? (2011)
  • Author(s): 橋爪一善、高橋透、木崎景一郎
  • Journal Title: HORMONE FRONTIER IN GYNECOLOGY Volume: 18 Pages: 259-267
  • Peer Reviewed
• [Journal Article] Cleaved bovine prolactin-related protein-I stimulates vascular endothelial cell proliferation (2010)
  • Author(s): Ushizawa K, Takahashi T, Hosoe M, Kizaki K, Hashizume K
  • Journal Title: Molecular and Cellular Endocrinology Volume: 323 Pages: 277-281
  • Peer Reviewed
• [Journal Article] (2010)
  • Author(s): Mishra B, Kizaki K, Koshi K, Ushizawa K, Takahashi T, Hosoe M, Sato T, Ito A, Hashizume K
  • Journal Title: Reproductive Biology and Endocrinology Volume: 8 Pages: 60-60
  • Peer Reviewed
• [Journal Article] Expression of extracellular matrix metalloproteinase inducer (EMMPRIN) and its related extracellular matrix degrading enzymes in the endometrium during estrous cycle and early gestation in cattle (2010)
  • Author(s): Mishra B, Kizaki K, Koshi K, Ushizawa K, Takahashi T, Hosoe M, Sato T, Ito A, Hashizume K
  • Journal Title: Reproductive Biology and Endocrinology Volume: 18 Pages: 60-60
  • Peer Reviewed
• [Presentation] ウシ抹消白血球に発現する妊娠シグナル (2013)
  • Author(s): 橋爪一善、木崎あゆみ、茂野智子、木崎景一郎、 松田秀雄、橋谷田 豊、今井 敬、古澤 軌、細江 実佐
  • Organizer: 第27回東日本家畜受精卵移植技術研究会大会(招待講演)
  • Place of Presentation: 栃木県宇都宮市
• [Presentation] ウシ抹消白血球に発現する妊娠シグナル (2013)
  • Author(s): 橋爪一善、木崎あゆみ、茂野智子、木崎景一郎、松田秀雄、橋谷田 豊、今井 敬、古澤 軌、細江実佐
  • Organizer: 第27回東日本家畜受精卵移植技術研究会大会
  • Place of Presentation: 宇都宮市
  • Invited
• [Presentation] Novel proteins affecting cell proliferation and angiogenesis of bovine placenta. (2011)
  • Author(s): Toru Takahashi, Koichi Ushizawa, Shobu Sasaki, Misa Hosoe, Keiichiro Kizaki and Kazuyoshi Hashizume
  • Organizer: International Meeting for Evolution of Reproductive Biology and Task of Frontiers: Trajectory and Prospects of IVF, Stem Cell and Epigenetic Studies
  • Place of Presentation: 岩手県盛岡市
• [Presentation] Novel proteins affecting cell proliferation and angiogenesis of bovine placenta (2011)
  • Author(s): Toru Takahashi, Koichi Ushizawa, Shobu Sasaki, Misa Hosoe, Keiichiro Kizaki, Kazuyoshi Hashizume
  • Organizer: International Meeting for Evolution of Reproductive Biology and Task of Frontiers : Trajectory and Prospects of IVF, Stem Cell and Epigenetic Studies
  • Place of Presentation: 岩手県盛岡市(招待講演)
• [Presentation] A novel factor for the regulation of utero-placental angiogenesis (2010)
  • Author(s): Takahashi T, Ushizawa K, Hosoe M, Kizaki K, Hashizume K
  • Organizer: SRD Symposium
  • Place of Presentation: 青森県十和田市
  • Year and Date: 2010-09-04
• [Presentation] Anovel factor for the regulation of utero-placental angiogenesis (2010)
  • Author(s): Takahashi T, Ushizawa K, Hosoe M, Kizaki K, Hashizume K
  • Organizer: SRD Symposium
  • Place of Presentation: 北里大学
  • Year and Date: 2010-09-04