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Developments for tick prevention using by baculovirus expressed with high-potential milk protein

Research Project

Project/Area Number 22580335
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Basic veterinary science/Basic zootechnical science
Research InstitutionKagoshima University

Principal Investigator

TANAKA Tetsuya  鹿児島大学, 共同獣医学部, 准教授 (00322842)

Co-Investigator(Kenkyū-buntansha) FUJISAKI Kozo  鹿児島大学, 農学部, 教授 (00292095)
Co-Investigator(Renkei-kenkyūsha) XUAN Xuennan  帯広畜産大学, 原虫病研究, センター (10292096)
Project Period (FY) 2010 – 2012
Project Status Completed (Fiscal Year 2012)
Budget Amount *help
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2012: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2011: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2010: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Keywordsラクトフェリン / トランスフェリン / フェリチン / マダニ / ラクトペルオキシダーゼ / バキュロウイルス
Research Abstract

W e hypothesized that high potential milk protein (HMP) might effect against the hatching and ovipositing of ticks. However, host blood-derived lactoferrin including in the HMP was not detected into tick after blood feeding. When we tried to detect host blood-derived transferrin which has the function aswell as lactoferrin, transferrin showed its unique transfer in tick by passing through the midgut until it reached the ovary. This suggest that host-derived transferrin plays a role in transporting iron to the ovary for embrygenesis.

Report

(4 results)
  • 2012 Annual Research Report   Final Research Report ( PDF )
  • 2011 Annual Research Report
  • 2010 Annual Research Report
  • Research Products

    (46 results)

All 2013 2012 2011 2010 Other

All Journal Article (10 results) (of which Peer Reviewed: 10 results) Presentation (31 results) Book (2 results) Remarks (3 results)

  • [Journal Article] Inhibitory effect of cyclophilin A from the hard tick Haemaphysalis longicornison the growth of Babesia bovisand Babesia bigemina2013

    • Author(s)
      Maeda, H., Boldbaatar, D., Kusakisako, K., Galay, RL., Aung, KM., Umemiya-Shirafuji, R., Mochizuki, M., Fujisaki, K., Tanaka, T
    • Journal Title

      Parasite Res

      Volume: 112 Issue: 6 Pages: 2207-2213

    • DOI

      10.1007/s00436-013-3390-7

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Multiple ferritins are vital to successful blood feeding and reproduction of the hard tick Haemaphysalis longicornis2013

    • Author(s)
      Galay, RL., Aung, KM., Umemiya-Shirafuji, R., Maeda, H., Matsuo, T., Kawaguchi, H., Miyoshi, N., Suzuki, H., Xuan, X., Mochizuki, M., Fujisaki, K., Tanaka, T
    • Journal Title

      J. Exp. Biol

      Volume: 216 Pages: 1905-1915

    • DOI

      10.1242/jeb.081240

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Multiple ferritins are vital to successful blood feeding and reproduction of the hard tick Haemaphysalis longicornis2013

    • Author(s)
      Galay RL et. al.
    • Journal Title

      J. Exp. Biol.

      Volume: In press

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Inhibitory effect of cyclophilin A from the hard tick Haemaphysalis longicornis on the growth of Babesia bovis and Babesia bigemina2013

    • Author(s)
      Maeda H et al.
    • Journal Title

      Parasite Res. (In press)

      Volume: In press

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Parasiticidal activity of Haemaphysalis longicornis longicin P4 peptide against Toxoplasma gondii2012

    • Author(s)
      Tanaka T, Maeda H, Matsuo T, Boldbattar D, Umemiya-Shirafuji R, Kume A, Suzuki H, Xuan X, Tsuji N, Fujisaki K.
    • Journal Title

      Peptides

      Volume: 34 Issue: 1 Pages: 242-250

    • DOI

      10.1016/j.peptides.2011.07.027

    • Related Report
      2012 Annual Research Report 2012 Final Research Report 2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Tick longicin implicated in the arthropod transmission of Toxoplasma gondii2012

    • Author(s)
      Tanaka, T., Maeda, H., Galay, RL., Boldbaatar, D., Umemiya-Shirafuji, R., Suzuki, H., Xuan, X., Tsuji, N., Fujisaki, K
    • Journal Title

      J. Veterinar. Sci. Technolo

      Volume: 03 Issue: 02 Pages: 112-112

    • DOI

      10.4172/2157-7579.1000112

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Anti-babesial activity of a potent peptide fragment derived from longicin of Haemaphysalis longicornis2012

    • Author(s)
      Galay, RL., Maeda, H., Aung, KM., Umemiya-Shirafuji, R., Xuan, X., Igarashi, I., Tsuji, N., Tanaka, T., Fujisaki, K
    • Journal Title

      Trop. Anim. Health Pro

      Volume: 44 Pages: 343-348

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Anti-babesial activity of a potent peptide fragment derived from longicin of Haemaphysalis longicornis2012

    • Author(s)
      Galay RL, Maeda H, Aung KM, Umemiya-Shirafuji R, Xuan X, IgarashiI, Tsuji N, Tanaka T, Fujisaki K.
    • Journal Title

      Trop Anim Health Prod

      Volume: 44 Issue: 2 Pages: 343-348

    • DOI

      10.1007/s11250-011-0027-7

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Tick longicin implicated in the arthropod transmission of Toxoplasma gondii2012

    • Author(s)
      Tanaka T et al.
    • Journal Title

      J. Veterinar. Sci. Technolo.

      Volume: 3 (2) Pages: 112-116

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Anti-babesial activity of a potent peptide fragment derived from longicin of Haemaplysalis longicornis2012

    • Author(s)
      Galay RL, et al
    • Journal Title

      Trop.Anim.Health Pro.

      Volume: 44(2) Pages: 343-348

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Presentation] The function of ferritin in iron transport、storage and protection against iron toxicity in the hard tick Haemaphysalis longicornis2013

    • Author(s)
      Remil Linggatong Galay, Kyaw Min Aung, Hiroki Maeda, Rika Umemiya-Shirafuji, Eugene Bacolod, Jiro Koyama, Masami Mochizuki, Kozo Fujisaki, Tetsuya Tanak
    • Organizer
      第82回日本寄生虫学会大会
    • Place of Presentation
      東京
    • Related Report
      2012 Final Research Report
  • [Presentation] フタトゲチマダニロンギシンにおける抗バベシア活性を持つペプチド部位の検索2013

    • Author(s)
      田仲哲也、Galay Remil Linggatong、前田大輝、Kyaw Min Aung、白藤(梅宮)梨可、玄 学南、五十嵐郁男、望月雅美、辻 尚利、藤崎幸蔵
    • Organizer
      第154回日本獣医学会学術集会
    • Place of Presentation
      東京
    • Related Report
      2012 Final Research Report
  • [Presentation] The function of ferritin in iron transport, storage and protection against iron toxicity in the hard tick Haemaphysalis longicornis2013

    • Author(s)
      Galay R et al.
    • Organizer
      第82回日本寄生虫学会大会
    • Place of Presentation
      東京医科歯科大学 (東京都)
    • Related Report
      2012 Annual Research Report
  • [Presentation] フタトゲチマダニにおける宿主血液由来トランスフェリンの動態について2012

    • Author(s)
      田仲哲也, ら
    • Organizer
      第153回日本獣医学会学術集会
    • Place of Presentation
      大宮ソニックシティ(さいたま市)
    • Year and Date
      2012-03-28
    • Related Report
      2011 Annual Research Report
  • [Presentation] Charactrization of two ferritin genes from Haemaphysalis longicornis, in particular, their role in blood feeding and reproduction2012

    • Author(s)
      Galay RL, et al
    • Organizer
      第81回日本寄生虫学会大会
    • Place of Presentation
      兵庫医科大学(西宮市)
    • Year and Date
      2012-03-24
    • Related Report
      2011 Annual Research Report
  • [Presentation] フタトゲチマダニの吸血および産卵におけるフェリチンの重要性2012

    • Author(s)
      Remil Linggatong Galay、Kyaw Min Aung、白藤(梅宮)梨可、前田大輝、松尾智英、川口博明、三好宣彰、鈴木宏志、玄 学南、望月雅美、藤崎幸蔵、田仲哲也
    • Organizer
      第35回日本分子生物学会年会
    • Place of Presentation
      福岡
    • Related Report
      2012 Final Research Report
  • [Presentation] フタトゲチマダニにおけるウサギ血液由来トランスフェリンの動態2012

    • Author(s)
      森寛之、Remil Linggatong Galay、前田大輝、松尾智英、白藤(梅宮)梨可、望月雅美、藤崎幸蔵、田仲哲也
    • Organizer
      第65回日本寄生虫学会南日本支部大会・第62回日本衛生動物学会南日本支部大会合同大会
    • Place of Presentation
      長崎
    • Related Report
      2012 Final Research Report
  • [Presentation] フタトゲチマダニ体内でのBabesia microtiの動態について2012

    • Author(s)
      草木迫浩大、前田大輝、Remil Linggatong Galay、松尾智英、白藤(梅宮)梨可、望月雅美、藤崎幸蔵、田仲哲也
    • Organizer
      第65回日本寄生虫学会南日本支部大会・第62回日本衛生動物学会南日本支部大会合同大会
    • Place of Presentation
      長崎
    • Related Report
      2012 Final Research Report
  • [Presentation] Characterization of two ferritin from Haemaphysalis longicornis、in particular their role in blood feeding and reproduction2012

    • Author(s)
      Remil Linggatong Galay, Kyaw Min Aung, Rika Umemiya-Shirafuji, Hiroki Maeda,Hiroshi Suzuki, Xuenan Xuan, Masami Mochizuki, Kozo Fujisaki, Tetsuya Tanaka
    • Organizer
      Chula International Workshop PEP2012
    • Place of Presentation
      タイ
    • Related Report
      2012 Final Research Report
  • [Presentation] フタトゲチマダニ由来サイクロフィリン A とバベシア原虫の関連性2012

    • Author(s)
      前田大輝, Damdinsuren Boldbaatar, 草木迫浩大, Kyaw Min Aung, Galay Remil, 白藤(梅宮)梨可, 望月雅美, 藤崎幸蔵, 田仲哲也
    • Organizer
      第154回日本獣医学会学術集会
    • Place of Presentation
      岩手
    • Related Report
      2012 Final Research Report
  • [Presentation] The importance of iron storage protein ferritin on successful blood feeding and reproduction of the hard tick Haemaphysalis longicornis2012

    • Author(s)
      Galay Remil, Kyaw Min Aung, 白藤(梅宮)梨可, 前田大輝, 松尾智英, 川口博明, 望月雅美, 藤崎幸蔵, 田仲哲也
    • Organizer
      第154回日本獣医学会学術集会
    • Place of Presentation
      岩手
    • Related Report
      2012 Final Research Report
  • [Presentation] Characterization and movement of iron binding proteins on Haemaphysalis longicornis2012

    • Author(s)
      Tetsuya Tanaka, Remil Linggatong Galay, Hiroyuki Mori, Kyaw Min Aung, Hiroki Maeda, Rika Umemiya-Shirafuji, Xuean Xuen, Hiroshi Suzuki, Masami Mochizuki, Kozo Fujisaki
    • Organizer
      XXIV International Congress of Entomology
    • Place of Presentation
      韓国
    • Related Report
      2012 Final Research Report
  • [Presentation] Charactrization of two ferritin genes from Haemaphysalis longicornis, in particular, their role in blood feeding and reproduction2012

    • Author(s)
      Remil Linggatong Galay, Kyaw Min Aung, Rika Umemiya-Shirafuji, Tetsuya Tanaka, Kozo Fujisaki
    • Organizer
      第81回日本寄生虫学会大会
    • Place of Presentation
      兵庫
    • Related Report
      2012 Final Research Report
  • [Presentation] フタトゲチマダニにおける宿主血液由来トランスフェリンの動態について2012

    • Author(s)
      田仲哲也、森 寛行、Galay Remil Linggatong、前田大輝、白藤(梅宮)梨可、藤崎幸蔵
    • Organizer
      第153回日本獣医学会学術集会
    • Place of Presentation
      埼玉
    • Related Report
      2012 Final Research Report
  • [Presentation] Characterization and movement of iron binding proteins on Haemaphysalis longicornis2012

    • Author(s)
      Tetsuya Tanaka et al.
    • Organizer
      XXIV International Congress of Entomology
    • Place of Presentation
      Exco (Daegu, Korea)
    • Related Report
      2012 Annual Research Report
  • [Presentation] The importance of iron storage protein ferritin on successful blood feeding and reproduction of the hard tick Haemaphysalis longicornis2012

    • Author(s)
      Galay R et al.
    • Organizer
      第154回日本獣医学会学術集会
    • Place of Presentation
      岩手大学 (岩手市)
    • Related Report
      2012 Annual Research Report
  • [Presentation] フタトゲチマダニ由来サイクロフィリンAとバベシア原虫の関連性2012

    • Author(s)
      前田大輝ら
    • Organizer
      第154回日本獣医学会学術集会
    • Place of Presentation
      岩手大学 (岩手市)
    • Related Report
      2012 Annual Research Report
  • [Presentation] Characterization of two ferritin from Haemaphysalis longicornis, in particular their role in blood feeding and reproduction2012

    • Author(s)
      Galay R et al.
    • Organizer
      Chula International Workshop PEP2012
    • Place of Presentation
      Chulalongkon university (Bangkok, Thailand)
    • Related Report
      2012 Annual Research Report
  • [Presentation] フタトゲチマダニにおけるウサギ血液由来トランスフェリンの動態2012

    • Author(s)
      森 寛之ら
    • Organizer
      第65回日本寄生虫学会南日本支部大会・第62回日本衛生動物学会南日本支部大会合同大会
    • Place of Presentation
      長崎大学 (長崎市)
    • Related Report
      2012 Annual Research Report
  • [Presentation] フタトゲチマダニの吸血および産卵におけるフェリチンの重要性2012

    • Author(s)
      Galay R et al.
    • Organizer
      第35回日本分子生物学会年会
    • Place of Presentation
      福岡国際会議場 (福岡市)
    • Related Report
      2012 Annual Research Report
  • [Presentation] Parasiticidal activity of tick longicin peptide2011

    • Author(s)
      Tetsuya Tanaka, et al
    • Organizer
      第34回日本分子生物学会年会
    • Place of Presentation
      パシフィコ横浜(横浜市)
    • Year and Date
      2011-12-16
    • Related Report
      2011 Annual Research Report
  • [Presentation] Parasiticidal activity of Haemaphysalis longicornis longicin P4 peptide against Toxoplasma gondii2011

    • Author(s)
      Tetsuya Tanaka, et al
    • Organizer
      17th Japanese-German Cooperative Symposium on Protozoan Diseases & Workshop of Gastrointestinal Protozoan Diseases
    • Place of Presentation
      Nara Women's University (Nara)
    • Year and Date
      2011-09-14
    • Related Report
      2011 Annual Research Report
  • [Presentation] Parasiticidal activity of tick longicin peptide2011

    • Author(s)
      Tetsuya Tanaka, Hiroki Maeda, Tomohide Matsuo, Remil Linggatong Galay, Damdinsuren Boldbattar, Rika Umemiya-Shirafuji, Hiroshi Suzuki, Xuenan Xuan, Naotoshi Tsuji, Kozo Fujisaki
    • Organizer
      第34回日本分子生物学会年会
    • Place of Presentation
      横浜
    • Related Report
      2012 Final Research Report
  • [Presentation] フタトゲチマダニ由来サイクロフィリン A の抗原虫作用2011

    • Author(s)
      前田大輝、田仲哲也、Damdinsuren Boldbaatar、草木迫浩太、Kyaw Min Aung、Remil Linggatong Galay、白藤(梅宮)梨可、玄学南、藤崎幸蔵
    • Organizer
      第64回日本寄生虫学会南日本支部大会・第61回日本衛生動物学会南日本支部大会合同大会
    • Place of Presentation
      宮崎
    • Related Report
      2012 Final Research Report
  • [Presentation] Parasiticidal activity of Haemaphysalis longicornislongicin P4 peptide against Toxoplasma gondii2011

    • Author(s)
      Tetsuya Tanaka, Hiroki Maeda, Tomohide Matsuo, Damdinsuren Boldbattar, Rika Umemiya-Shirafuji, Aiko Kume, Hiroshi Suzuki, Xuenan Xuan, Naotoshi Tsuji, Kozo Fujisaki
    • Organizer
      17th Japanese-German Cooperative Symposium on Protozoan Diseases &Workshop of Gastrointestinal Protozoan Diseases
    • Place of Presentation
      日本
    • Related Report
      2012 Final Research Report
  • [Presentation] Parasiticidal activity of Haemaphysalis longicornislongicin against Toxoplasma gondii2011

    • Author(s)
      Tetsuya Tanaka, Hiroki Maeda, Tomohide Matsuo, Damdinsuren Boldbattar, Rika Umemiya-Shirafuji, Aiko Kume, Hiroshi Suzuki, Xuenan Xuan, Naotoshi Tsuji, Kozo Fujisaki
    • Organizer
      TTP7: Ticks and Tick-Borne Pathogens
    • Place of Presentation
      スペイン
    • Related Report
      2012 Final Research Report
  • [Presentation] フタトゲチマダニロンギシンのトキソプラズマ原虫に及ぼす影響について2011

    • Author(s)
      田仲哲也、前田大輝、松尾智英、Damidinsuren Boldbaatar、白藤(梅宮)梨可、玄学南、 辻尚利、藤崎幸蔵
    • Organizer
      第151回日本獣医学会学術集会
    • Place of Presentation
      東京
    • Related Report
      2012 Final Research Report
  • [Presentation] Parasiticidal activity of Haemaphysalis longicornis longicin against Toxoplasma gondii2011

    • Author(s)
      Tetsuya Tanaka, et al
    • Organizer
      TTP7 : Ticks and Tick-Borne Pathogens
    • Place of Presentation
      City Auditorium (Zaragoza, Spin)
    • Related Report
      2011 Annual Research Report
  • [Presentation] フタトゲチマダニロンギシンP4のトキソプラズマに対する抗原虫作用2010

    • Author(s)
      田仲哲也, ら
    • Organizer
      第33回日本分子生物学会年会・第83回日本生化学会大会合同大会
    • Place of Presentation
      神戸国際会議場(神戸市)
    • Year and Date
      2010-12-10
    • Related Report
      2010 Annual Research Report
  • [Presentation] フタトゲチマダニロンギシン P4 のトキソプラズマに対する抗原虫作用2010

    • Author(s)
      田仲哲也、松尾智英、Md. Morshedur Rahman、Boldbaatar Damdinsuren、白藤(梅宮)梨可、玄学南、辻尚利、藤崎幸蔵
    • Organizer
      第33回日本分子生物学会年会・第83回日本生化学会大会合同大会
    • Place of Presentation
      兵庫
    • Related Report
      2012 Final Research Report
  • [Presentation] フタトゲチマダニロンギシン P4 のトキソプラズマにおける影響2010

    • Author(s)
      田仲哲也、 前田大輝、 松尾智英、 Md. Morshedur Rahman 、 Boldbaatar Damdinsuren、白藤(梅宮)梨可、玄学南、 辻尚利、 藤崎幸蔵
    • Organizer
      第63回日本寄生虫学会南日本支部大会・第60回日本衛生動物学会南日本支部大会合同大会
    • Place of Presentation
      鹿児島
    • Related Report
      2012 Final Research Report
  • [Book] in Microbial pathogens and strategies for combating them2013

    • Author(s)
      Tanaka, T., Fujisaki, K
    • Publisher
      science, technology and education ed. By A. Mendez-Vilas.
    • Related Report
      2012 Final Research Report
  • [Book] Expression and characterization of bovine milk antimicrobial proteins lactoperoxidase and lactoferrin by vaccinia virus2012

    • Author(s)
      Tanaka, T., Xuan X., Fujisaki, K., Shimazaki, K
    • Publisher
      InTech-Open Access
    • Related Report
      2012 Final Research Report
  • [Remarks] 鹿児島大学 共同獣医学部 新興感染症学分野 ホームページ

    • URL

      http://www.vet.kagoshima-u.ac.jp/kadai/V-infection/sinkoukansen/top.html

    • Related Report
      2012 Annual Research Report
  • [Remarks]

    • URL

      http://w3vet.agri.kagoshima-u.ac.jp/V-Infection/sinkoukansen/toppage.html

    • Related Report
      2011 Annual Research Report
  • [Remarks]

    • URL

      http://w3vet.agri.kagoshima-u.ac.jp/V-Infection/sinkoukansen/toppage.html

    • Related Report
      2010 Annual Research Report

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Published: 2010-08-23   Modified: 2019-07-29  

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