Developments for tick prevention using by baculovirus expressed with high-potential milk protein

• Project/Area Number: 22580335
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Basic veterinary science/Basic zootechnical science
• Research Institution: Kagoshima University
• Principal Investigator: TANAKA Tetsuya 鹿児島大学, 共同獣医学部, 准教授 (00322842)
• Co-Investigator(Kenkyū-buntansha): FUJISAKI Kozo 鹿児島大学, 農学部, 教授 (00292095)
• Co-Investigator(Renkei-kenkyūsha): XUAN Xuennan 帯広畜産大学, 原虫病研究, センター (10292096)
• Project Period (FY): 2010 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2012: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2011: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2010: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
• Keywords: ラクトフェリン / トランスフェリン / フェリチン / マダニ / ラクトペルオキシダーゼ / バキュロウイルス

Research Abstract

W e hypothesized that high potential milk protein (HMP) might effect against the hatching and ovipositing of ticks. However, host blood-derived lactoferrin including in the HMP was not detected into tick after blood feeding. When we tried to detect host blood-derived transferrin which has the function aswell as lactoferrin, transferrin showed its unique transfer in tick by passing through the midgut until it reached the ovary. This suggest that host-derived transferrin plays a role in transporting iron to the ovary for embrygenesis.

Research Products

• [Journal Article] Inhibitory effect of cyclophilin A from the hard tick Haemaphysalis longicornison the growth of Babesia bovisand Babesia bigemina (2013)
  • Author(s): Maeda, H., Boldbaatar, D., Kusakisako, K., Galay, RL., Aung, KM., Umemiya-Shirafuji, R., Mochizuki, M., Fujisaki, K., Tanaka, T
  • Journal Title: Parasite Res Volume: 112 Issue: 6 Pages: 2207-2213
  • DOI: 10.1007/s00436-013-3390-7
  • Peer Reviewed
• [Journal Article] Multiple ferritins are vital to successful blood feeding and reproduction of the hard tick Haemaphysalis longicornis (2013)
  • Author(s): Galay, RL., Aung, KM., Umemiya-Shirafuji, R., Maeda, H., Matsuo, T., Kawaguchi, H., Miyoshi, N., Suzuki, H., Xuan, X., Mochizuki, M., Fujisaki, K., Tanaka, T
  • Journal Title: J. Exp. Biol Volume: 216 Pages: 1905-1915
  • DOI: 10.1242/jeb.081240
  • Peer Reviewed
• [Journal Article] Multiple ferritins are vital to successful blood feeding and reproduction of the hard tick Haemaphysalis longicornis (2013)
  • Author(s): Galay RL et. al.
  • Journal Title: J. Exp. Biol. Volume: In press
  • Peer Reviewed
• [Journal Article] Inhibitory effect of cyclophilin A from the hard tick Haemaphysalis longicornis on the growth of Babesia bovis and Babesia bigemina (2013)
  • Author(s): Maeda H et al.
  • Journal Title: Parasite Res. (In press) Volume: In press
  • Peer Reviewed
• [Journal Article] Parasiticidal activity of Haemaphysalis longicornis longicin P4 peptide against Toxoplasma gondii (2012)
  • Author(s): Tanaka T, Maeda H, Matsuo T, Boldbattar D, Umemiya-Shirafuji R, Kume A, Suzuki H, Xuan X, Tsuji N, Fujisaki K.
  • Journal Title: Peptides Volume: 34 Issue: 1 Pages: 242-250
  • DOI: 10.1016/j.peptides.2011.07.027
  • Peer Reviewed
• [Journal Article] Tick longicin implicated in the arthropod transmission of Toxoplasma gondii (2012)
  • Author(s): Tanaka, T., Maeda, H., Galay, RL., Boldbaatar, D., Umemiya-Shirafuji, R., Suzuki, H., Xuan, X., Tsuji, N., Fujisaki, K
  • Journal Title: J. Veterinar. Sci. Technolo Volume: 03 Issue: 02 Pages: 112-112
  • DOI: 10.4172/2157-7579.1000112
  • Peer Reviewed
• [Journal Article] Anti-babesial activity of a potent peptide fragment derived from longicin of Haemaphysalis longicornis (2012)
  • Author(s): Galay, RL., Maeda, H., Aung, KM., Umemiya-Shirafuji, R., Xuan, X., Igarashi, I., Tsuji, N., Tanaka, T., Fujisaki, K
  • Journal Title: Trop. Anim. Health Pro Volume: 44 Pages: 343-348
  • Peer Reviewed
• [Journal Article] Anti-babesial activity of a potent peptide fragment derived from longicin of Haemaphysalis longicornis (2012)
  • Author(s): Galay RL, Maeda H, Aung KM, Umemiya-Shirafuji R, Xuan X, IgarashiI, Tsuji N, Tanaka T, Fujisaki K.
  • Journal Title: Trop Anim Health Prod Volume: 44 Issue: 2 Pages: 343-348
  • DOI: 10.1007/s11250-011-0027-7
  • Peer Reviewed
• [Journal Article] Tick longicin implicated in the arthropod transmission of Toxoplasma gondii (2012)
  • Author(s): Tanaka T et al.
  • Journal Title: J. Veterinar. Sci. Technolo. Volume: 3 (2) Pages: 112-116
  • Peer Reviewed
• [Journal Article] Anti-babesial activity of a potent peptide fragment derived from longicin of Haemaplysalis longicornis (2012)
  • Author(s): Galay RL, et al
  • Journal Title: Trop.Anim.Health Pro. Volume: 44(2) Pages: 343-348
  • Peer Reviewed
• [Presentation] The function of ferritin in iron transport、storage and protection against iron toxicity in the hard tick Haemaphysalis longicornis (2013)
  • Author(s): Remil Linggatong Galay, Kyaw Min Aung, Hiroki Maeda, Rika Umemiya-Shirafuji, Eugene Bacolod, Jiro Koyama, Masami Mochizuki, Kozo Fujisaki, Tetsuya Tanak
  • Organizer: 第82回日本寄生虫学会大会
  • Place of Presentation: 東京
• [Presentation] フタトゲチマダニロンギシンにおける抗バベシア活性を持つペプチド部位の検索 (2013)
  • Author(s): 田仲哲也、Galay Remil Linggatong、前田大輝、Kyaw Min Aung、白藤(梅宮)梨可、玄 学南、五十嵐郁男、望月雅美、辻 尚利、藤崎幸蔵
  • Organizer: 第154回日本獣医学会学術集会
  • Place of Presentation: 東京
• [Presentation] The function of ferritin in iron transport, storage and protection against iron toxicity in the hard tick Haemaphysalis longicornis (2013)
  • Author(s): Galay R et al.
  • Organizer: 第82回日本寄生虫学会大会
  • Place of Presentation: 東京医科歯科大学 (東京都)
• [Presentation] フタトゲチマダニにおける宿主血液由来トランスフェリンの動態について (2012)
  • Author(s): 田仲哲也, ら
  • Organizer: 第153回日本獣医学会学術集会
  • Place of Presentation: 大宮ソニックシティ(さいたま市)
  • Year and Date: 2012-03-28
• [Presentation] Charactrization of two ferritin genes from Haemaphysalis longicornis, in particular, their role in blood feeding and reproduction (2012)
  • Author(s): Galay RL, et al
  • Organizer: 第81回日本寄生虫学会大会
  • Place of Presentation: 兵庫医科大学(西宮市)
  • Year and Date: 2012-03-24
• [Presentation] フタトゲチマダニの吸血および産卵におけるフェリチンの重要性 (2012)
  • Author(s): Remil Linggatong Galay、Kyaw Min Aung、白藤(梅宮)梨可、前田大輝、松尾智英、川口博明、三好宣彰、鈴木宏志、玄 学南、望月雅美、藤崎幸蔵、田仲哲也
  • Organizer: 第35回日本分子生物学会年会
  • Place of Presentation: 福岡
• [Presentation] フタトゲチマダニにおけるウサギ血液由来トランスフェリンの動態 (2012)
  • Author(s): 森寛之、Remil Linggatong Galay、前田大輝、松尾智英、白藤(梅宮)梨可、望月雅美、藤崎幸蔵、田仲哲也
  • Organizer: 第65回日本寄生虫学会南日本支部大会・第62回日本衛生動物学会南日本支部大会合同大会
  • Place of Presentation: 長崎
• [Presentation] フタトゲチマダニ体内でのBabesia microtiの動態について (2012)
  • Author(s): 草木迫浩大、前田大輝、Remil Linggatong Galay、松尾智英、白藤(梅宮)梨可、望月雅美、藤崎幸蔵、田仲哲也
  • Organizer: 第65回日本寄生虫学会南日本支部大会・第62回日本衛生動物学会南日本支部大会合同大会
  • Place of Presentation: 長崎
• [Presentation] Characterization of two ferritin from Haemaphysalis longicornis、in particular their role in blood feeding and reproduction (2012)
  • Author(s): Remil Linggatong Galay, Kyaw Min Aung, Rika Umemiya-Shirafuji, Hiroki Maeda,Hiroshi Suzuki, Xuenan Xuan, Masami Mochizuki, Kozo Fujisaki, Tetsuya Tanaka
  • Organizer: Chula International Workshop PEP2012
  • Place of Presentation: タイ
• [Presentation] フタトゲチマダニ由来サイクロフィリン A とバベシア原虫の関連性 (2012)
  • Author(s): 前田大輝, Damdinsuren Boldbaatar, 草木迫浩大, Kyaw Min Aung, Galay Remil, 白藤(梅宮)梨可, 望月雅美, 藤崎幸蔵, 田仲哲也
  • Organizer: 第154回日本獣医学会学術集会
  • Place of Presentation: 岩手
• [Presentation] The importance of iron storage protein ferritin on successful blood feeding and reproduction of the hard tick Haemaphysalis longicornis (2012)
  • Author(s): Galay Remil, Kyaw Min Aung, 白藤(梅宮)梨可, 前田大輝, 松尾智英, 川口博明, 望月雅美, 藤崎幸蔵, 田仲哲也
  • Organizer: 第154回日本獣医学会学術集会
  • Place of Presentation: 岩手
• [Presentation] Characterization and movement of iron binding proteins on Haemaphysalis longicornis (2012)
  • Author(s): Tetsuya Tanaka, Remil Linggatong Galay, Hiroyuki Mori, Kyaw Min Aung, Hiroki Maeda, Rika Umemiya-Shirafuji, Xuean Xuen, Hiroshi Suzuki, Masami Mochizuki, Kozo Fujisaki
  • Organizer: XXIV International Congress of Entomology
  • Place of Presentation: 韓国
• [Presentation] Charactrization of two ferritin genes from Haemaphysalis longicornis, in particular, their role in blood feeding and reproduction (2012)
  • Author(s): Remil Linggatong Galay, Kyaw Min Aung, Rika Umemiya-Shirafuji, Tetsuya Tanaka, Kozo Fujisaki
  • Organizer: 第81回日本寄生虫学会大会
  • Place of Presentation: 兵庫
• [Presentation] フタトゲチマダニにおける宿主血液由来トランスフェリンの動態について (2012)
  • Author(s): 田仲哲也、森 寛行、Galay Remil Linggatong、前田大輝、白藤(梅宮)梨可、藤崎幸蔵
  • Organizer: 第153回日本獣医学会学術集会
  • Place of Presentation: 埼玉
• [Presentation] Characterization and movement of iron binding proteins on Haemaphysalis longicornis (2012)
  • Author(s): Tetsuya Tanaka et al.
  • Organizer: XXIV International Congress of Entomology
  • Place of Presentation: Exco (Daegu, Korea)
• [Presentation] The importance of iron storage protein ferritin on successful blood feeding and reproduction of the hard tick Haemaphysalis longicornis (2012)
  • Author(s): Galay R et al.
  • Organizer: 第154回日本獣医学会学術集会
  • Place of Presentation: 岩手大学 (岩手市)
• [Presentation] フタトゲチマダニ由来サイクロフィリンAとバベシア原虫の関連性 (2012)
  • Author(s): 前田大輝ら
  • Organizer: 第154回日本獣医学会学術集会
  • Place of Presentation: 岩手大学 (岩手市)
• [Presentation] Characterization of two ferritin from Haemaphysalis longicornis, in particular their role in blood feeding and reproduction (2012)
  • Author(s): Galay R et al.
  • Organizer: Chula International Workshop PEP2012
  • Place of Presentation: Chulalongkon university (Bangkok, Thailand)
• [Presentation] フタトゲチマダニにおけるウサギ血液由来トランスフェリンの動態 (2012)
  • Author(s): 森 寛之ら
  • Organizer: 第65回日本寄生虫学会南日本支部大会・第62回日本衛生動物学会南日本支部大会合同大会
  • Place of Presentation: 長崎大学 (長崎市)
• [Presentation] フタトゲチマダニの吸血および産卵におけるフェリチンの重要性 (2012)
  • Author(s): Galay R et al.
  • Organizer: 第35回日本分子生物学会年会
  • Place of Presentation: 福岡国際会議場 (福岡市)
• [Presentation] Parasiticidal activity of tick longicin peptide (2011)
  • Author(s): Tetsuya Tanaka, et al
  • Organizer: 第34回日本分子生物学会年会
  • Place of Presentation: パシフィコ横浜(横浜市)
  • Year and Date: 2011-12-16
• [Presentation] Parasiticidal activity of Haemaphysalis longicornis longicin P4 peptide against Toxoplasma gondii (2011)
  • Author(s): Tetsuya Tanaka, et al
  • Organizer: 17th Japanese-German Cooperative Symposium on Protozoan Diseases & Workshop of Gastrointestinal Protozoan Diseases
  • Place of Presentation: Nara Women's University (Nara)
  • Year and Date: 2011-09-14
• [Presentation] Parasiticidal activity of tick longicin peptide (2011)
  • Author(s): Tetsuya Tanaka, Hiroki Maeda, Tomohide Matsuo, Remil Linggatong Galay, Damdinsuren Boldbattar, Rika Umemiya-Shirafuji, Hiroshi Suzuki, Xuenan Xuan, Naotoshi Tsuji, Kozo Fujisaki
  • Organizer: 第34回日本分子生物学会年会
  • Place of Presentation: 横浜
• [Presentation] フタトゲチマダニ由来サイクロフィリン A の抗原虫作用 (2011)
  • Author(s): 前田大輝、田仲哲也、Damdinsuren Boldbaatar、草木迫浩太、Kyaw Min Aung、Remil Linggatong Galay、白藤(梅宮)梨可、玄学南、藤崎幸蔵
  • Organizer: 第64回日本寄生虫学会南日本支部大会・第61回日本衛生動物学会南日本支部大会合同大会
  • Place of Presentation: 宮崎
• [Presentation] Parasiticidal activity of Haemaphysalis longicornislongicin P4 peptide against Toxoplasma gondii (2011)
  • Author(s): Tetsuya Tanaka, Hiroki Maeda, Tomohide Matsuo, Damdinsuren Boldbattar, Rika Umemiya-Shirafuji, Aiko Kume, Hiroshi Suzuki, Xuenan Xuan, Naotoshi Tsuji, Kozo Fujisaki
  • Organizer: 17th Japanese-German Cooperative Symposium on Protozoan Diseases &Workshop of Gastrointestinal Protozoan Diseases
  • Place of Presentation: 日本
• [Presentation] Parasiticidal activity of Haemaphysalis longicornislongicin against Toxoplasma gondii (2011)
  • Author(s): Tetsuya Tanaka, Hiroki Maeda, Tomohide Matsuo, Damdinsuren Boldbattar, Rika Umemiya-Shirafuji, Aiko Kume, Hiroshi Suzuki, Xuenan Xuan, Naotoshi Tsuji, Kozo Fujisaki
  • Organizer: TTP7: Ticks and Tick-Borne Pathogens
  • Place of Presentation: スペイン
• [Presentation] フタトゲチマダニロンギシンのトキソプラズマ原虫に及ぼす影響について (2011)
  • Author(s): 田仲哲也、前田大輝、松尾智英、Damidinsuren Boldbaatar、白藤(梅宮)梨可、玄学南、 辻尚利、藤崎幸蔵
  • Organizer: 第151回日本獣医学会学術集会
  • Place of Presentation: 東京
• [Presentation] Parasiticidal activity of Haemaphysalis longicornis longicin against Toxoplasma gondii (2011)
  • Author(s): Tetsuya Tanaka, et al
  • Organizer: TTP7 : Ticks and Tick-Borne Pathogens
  • Place of Presentation: City Auditorium (Zaragoza, Spin)
• [Presentation] フタトゲチマダニロンギシンP4のトキソプラズマに対する抗原虫作用 (2010)
  • Author(s): 田仲哲也, ら
  • Organizer: 第33回日本分子生物学会年会・第83回日本生化学会大会合同大会
  • Place of Presentation: 神戸国際会議場(神戸市)
  • Year and Date: 2010-12-10
• [Presentation] フタトゲチマダニロンギシン P4 のトキソプラズマに対する抗原虫作用 (2010)
  • Author(s): 田仲哲也、松尾智英、Md. Morshedur Rahman、Boldbaatar Damdinsuren、白藤(梅宮)梨可、玄学南、辻尚利、藤崎幸蔵
  • Organizer: 第33回日本分子生物学会年会・第83回日本生化学会大会合同大会
  • Place of Presentation: 兵庫
• [Presentation] フタトゲチマダニロンギシン P4 のトキソプラズマにおける影響 (2010)
  • Author(s): 田仲哲也、 前田大輝、 松尾智英、 Md. Morshedur Rahman 、 Boldbaatar Damdinsuren、白藤(梅宮)梨可、玄学南、 辻尚利、 藤崎幸蔵
  • Organizer: 第63回日本寄生虫学会南日本支部大会・第60回日本衛生動物学会南日本支部大会合同大会
  • Place of Presentation: 鹿児島
• [Book] in Microbial pathogens and strategies for combating them (2013)
  • Author(s): Tanaka, T., Fujisaki, K
  • Publisher: science, technology and education ed. By A. Mendez-Vilas.
• [Book] Expression and characterization of bovine milk antimicrobial proteins lactoperoxidase and lactoferrin by vaccinia virus (2012)
  • Author(s): Tanaka, T., Xuan X., Fujisaki, K., Shimazaki, K
  • Publisher: InTech-Open Access
• [Remarks] 鹿児島大学 共同獣医学部 新興感染症学分野 ホームページ
  • URL: http://www.vet.kagoshima-u.ac.jp/kadai/V-infection/sinkoukansen/top.html
• [Remarks]
  • URL: http://w3vet.agri.kagoshima-u.ac.jp/V-Infection/sinkoukansen/toppage.html
• [Remarks]
  • URL: http://w3vet.agri.kagoshima-u.ac.jp/V-Infection/sinkoukansen/toppage.html