Research for therapeutic application of dominant negative GAPDH molecule on oxidative stress-induced disorder
| Project/Area Number |
22580339
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Basic veterinary science/Basic zootechnical science
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| Research Institution | Osaka Prefecture University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
KUWAMURA Mitsuru 大阪府立大学, 生命環境科学研究科, 准教授 (20244668)
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| Co-Investigator(Renkei-kenkyūsha) |
HIKIDA Takatoshi (財)大阪バイオサイエンス研究所, システムズ生物学部門, 研究員 (70421378)
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| Project Period (FY) |
2010 – 2012
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| Project Status |
Completed (Fiscal Year 2012)
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| Budget Amount *help |
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2012: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2011: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2010: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Keywords | 薬理 / 神経変性疾患 / トランスジェニックマウス / 酸化ストレス / 治療 / 疾病予防・制御 / 新規治療薬開発 / GAPDH / 脳神経疾患 / ドミナントネガティブ分子 / アミロイド |
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| Research Abstract |
In the present study, we aim to establish a conditional transgenic mice containing mutant GAPDH (C152A-GPDH), which inhibits oxidative stress-induced cell death mediated by GAPDH aggregation dominant negatively, and to investigate whether therapeutic benefits of C152A-GAPDH might be showedin vivo. As a result, we have succeeded two strains of mice in which the expression of C152A-GAPDH is under the control of a tetracycline-inducible system. And then, we are examining the possibility for therapeutic application of C152A-GAPDH on oxidative stress-induced disorders such as stroke model or methamphetamine-elicited Parkinson disease-like model using the established transgenic mice, compared to that of wild-type mice.
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Report
(4 results)
Research Products
(27 results)
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[Journal Article] A rapid, targeted, neuron-selective, in vivo knockdown following a single intracerebroventricular injection of a novel chemically modified siRNA in the adult rat brain2012
Author(s)
Nakajima H, Kubo T, Semi Y, Itakura M, Kuwamura M, Izawa T, Azuma YT, Takeuchi T
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Journal Title
Journal of Biotechnology
Volume: 152(2)
Issue: 2
Pages: 326-333
DOI
Related Report
Peer Reviewed
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