| Project/Area Number |
22580340
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Basic veterinary science/Basic zootechnical science
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| Research Institution | Nihon University |
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Principal Investigator |
KANO Koichiro 日本大学, 生物資源科学部, 准教授 (80271039)
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| Project Period (FY) |
2010 – 2012
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| Project Status |
Completed (Fiscal Year 2012)
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| Budget Amount *help |
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2012: ¥260,000 (Direct Cost: ¥200,000、Indirect Cost: ¥60,000)
Fiscal Year 2011: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
Fiscal Year 2010: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | 発生 / 多能性獲得 / 脱分化 / 脱分化脂肪細胞 / DFAT / バイオインフォマティクス / プロトプラスト / 脂肪細胞 / 卵胞顆粒層細胞 / 成熟脂肪細胞 |
|---|
| Research Abstract |
We conducted global gene expression analyses to clarify the dedifferentiationmechanism of mature adipocytes(MAs) and follicular granulosa cells(GCs). By comparingglobal gene expression profiles during the dedifferentiation process, we searched for particular biological functions in those genes whose expression intensities wereincreased or decreased by dedifferentiation of MAs and GCs. After dedifferentiation,expression of those genes expressed strongly in MAs and GCs, such as genes for lipidmetabolism and reproductive system development, was remarkably decreased. Moreover, in the proeess of dedifferentiation of both MAs and GCs, genes involved in cell morphology,cytoskeleton, cell adhesion, angiogenesis, cell proliferation, and tissue developmentwere upregulated, whereas those involved in the regulation of gene expression weredownregulated.
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