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Global study using fluorescent Neospora caninum on the vertical transmission mechanism in neosporosis

Research Project

Project/Area Number 22580342
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Basic veterinary science/Basic zootechnical science
Research InstitutionNippon Veterinary and Life Science University

Principal Investigator

IKE Kazunori  日本獣医生命科学大学, 獣医学部・獣医学科, 准教授 (50159597)

Co-Investigator(Kenkyū-buntansha) IMAI Soichi  日本獣医生命科学大学, 獣医学部・獣医学科, 教授 (90120758)
Project Period (FY) 2010 – 2012
Project Status Completed (Fiscal Year 2012)
Budget Amount *help
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2012: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2011: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2010: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Keywords寄生虫 / Neospora caninum / ネオスポラ症 / 垂直感染 / 蛍光発色原虫 / ワクチン
Research Abstract

At first we visualized Neospora caninum by making a fluorescent color development this protozoan and facilitated various kinds of experiments of neosporosis in this study. We identified the gene encoded the NcBAG1 protein which could become the marker of bradyzoite stage. Furthermore, we confirmed the effect of this protein in the horizontal transmitted mouse model of the antigen derived from bradyzoite. The mixed vaccine of the both antigens showed synergy effects against the horizontal transmission, but the effect was not found in the vertical transmission. We were not able to confirm the effect of the estrogen in the vertical transmission in this study.

Report

(4 results)
  • 2012 Annual Research Report   Final Research Report ( PDF )
  • 2011 Annual Research Report
  • 2010 Annual Research Report
  • Research Products

    (22 results)

All 2013 2012 2011 2010

All Journal Article (12 results) (of which Peer Reviewed: 12 results) Presentation (9 results) Book (1 results)

  • [Journal Article] Comparative study of protective abilities of NcBAG1, NcBSR4, NcMAG1, and NcSAG4 antigens against Neospora caninum infection in acute mouse model.2013

    • Author(s)
      Uchida, M., Nagashima, K., Akatsuka, Y., Ito, A., Imai, S., Ike, K.
    • Journal Title

      Parasitol. Res.

      Volume: 112 Pages: 655-663

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Development 1 of two murine antibodies against Neospora caninum using phage display technology and application on the detection of neosporosis.2013

    • Author(s)
      Dong, J., Otsuki, T., Kato, T., Kohsaka, T., Ike, K., Park, E. Y.
    • Journal Title

      PlosOne

      Volume: 8

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Gene cloning and characterization of the protein encoded by the Neospora caninum bradyzoite-specific antigengene BAG1.2013

    • Author(s)
      Kobayashi, T., Narabu, S., Yanai, Y., Hatano, Y., Ito, A., Imai, S., Ike, K.
    • Journal Title

      J. Parasitol.

      Volume: 99 Pages: 453-458

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Comparative study of protective abilities of NcBAG1, NcBSR4, NcMAG1, and NcSAG4 antigens against Neospora caninum infection in acute mouse model2013

    • Author(s)
      Masaki Uchida
    • Journal Title

      Parasitology Research

      Volume: 112 Issue: 2 Pages: 655-663

    • DOI

      10.1007/s00436-012-3182-5

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Development 1 of two murine antibodies against Neospora caninum using phage display technology and application on the detection of neosporosis2013

    • Author(s)
      Jinhua Dong
    • Journal Title

      PlosOne

      Volume: 8

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Detection of Nesopora caninum-specific DNA from cerebrospinal fluid by polymerase chain reaction in a dog with confirmed neosporosis.2012

    • Author(s)
      Ishigaki, K., Noya, M., Kagawa, Y., Ike, K. , Orima, H., Imai, S.
    • Journal Title

      J. Vet. Med. Sci.

      Volume: 74 Pages: 1051-1055

    • NAID

      130001879790

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Induction of T Helper 1 (Th1) immune response by an extract from Pleurotus eryngii (Eringi).2012

    • Author(s)
      Ike, K., Kameyama, N., Ito, A., Imai, S.
    • Journal Title

      J. Med. Food

      Volume: 15 Pages: 1124-1128

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Detection of Nesopora caninum-specific DNA from cerebrospinal fluid by polymerase chain reaction in a dog with confirmed neosporosis2012

    • Author(s)
      Kyohei Ishigaki
    • Journal Title

      The Journal of Veterinary Medical Science

      Volume: 74 Pages: 1051-1055

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Induction of T Helper 1 (Th1) immune response by an extract from Pleurotus eryngii (Eringi)2012

    • Author(s)
      Kazunori Ike
    • Journal Title

      Journal of Medical Food

      Volume: 15 Pages: 1124-1128

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Infraciliature of eight Triplumaria species (Ciliophora, Entodiniomorphida) from Asian elephants with the description of six new species2011

    • Author(s)
      Ito, A., Mishima.T., Nataami, K., Ike, K., Imai, S.
    • Journal Title

      Eur.J.Protistol.

      Volume: 47 Issue: 4 Pages: 256-273

    • DOI

      10.1016/j.ejop.2011.06.002

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Influence of zinc deficiency to the mice infected with Bahesia microti2011

    • Author(s)
      Hamaguchi K, Ike K, Yamazaki M, Morita T, Imai S
    • Journal Title

      Journal of Veterinary Medical Science

      Volume: 73 Pages: 263-267

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Bartonella species in wild rodents and fleas from them in Japan2011

    • Author(s)
      Kabeya H, Inoue K, Izumi Y, Morita T, Imai, S, Maruyama S
    • Journal Title

      Journal of Veterinary Medical Science

      Volume: 73 Pages: 1561-1567

    • NAID

      130001032749

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Presentation] マウスモデルにおけるNeospora caninumタキゾイトおよびブラディゾイト由来抗原の防御に関する相乗的効果2012

    • Author(s)
      永島琴美、池和憲、小林哲也、赤塚唯、沢村洋緒、栗原佑生子、松本裕美乃、森田達志、今井壮一
    • Organizer
      第154回日本獣医学会学術集会
    • Place of Presentation
      盛岡市
    • Year and Date
      2012-09-15
    • Related Report
      2012 Final Research Report
  • [Presentation] マウスモデルにおけるNeospora caninumタキゾイトおよびブラディゾイト由来抗原の防御に関する相乗的効果2012

    • Author(s)
      永島琴美
    • Organizer
      第154回日本獣医学会
    • Place of Presentation
      岩手大学(岩手県)
    • Related Report
      2012 Annual Research Report
  • [Presentation] ホンシュウジカに寄生するSarcocystisの分類2011

    • Author(s)
      木本佳那・松尾史朗・青木杏津沙・菊田基・森田達志・池和憲・今井壮一
    • Organizer
      第44回日本原生動物学会
    • Place of Presentation
      奈良女子大学(奈良市)
    • Related Report
      2011 Annual Research Report
  • [Presentation] Neospora caninum中間宿主ステージ特異的発色原虫の作出2010

    • Author(s)
      羽多野優芳、池和憲、内田昌樹、赤塚唯、永島琴美、森田達志、今井壮一
    • Organizer
      第150回日本獣医学会学術集会
    • Place of Presentation
      帯広市
    • Year and Date
      2010-09-18
    • Related Report
      2012 Final Research Report
  • [Presentation] Neospora caninumブラディゾイト由来抗原による防御能のマウスモデルにおける検討2010

    • Author(s)
      内田昌樹、池和憲、羽多野優芳、赤塚唯、永島琴美、森田達志、今井壮一
    • Organizer
      第150回日本獣医学会学術集会
    • Place of Presentation
      帯広市
    • Year and Date
      2010-09-18
    • Related Report
      2012 Final Research Report
  • [Presentation] 臨床症状を伴った犬ネオスポラ症の一例2010

    • Author(s)
      石垣恭平、池和憲、野矢雅彦、賀川由美子、織間博光、今井壮一
    • Organizer
      第150回日本獣医学会学術集会
    • Place of Presentation
      帯広市
    • Year and Date
      2010-09-18
    • Related Report
      2012 Final Research Report
  • [Presentation] 臨床症状を伴った犬ネオスポラ症の一例2010

    • Author(s)
      池和憲
    • Organizer
      第150回日本獣医学会学術集会
    • Place of Presentation
      帯広畜産大学(北海道)
    • Year and Date
      2010-09-18
    • Related Report
      2010 Annual Research Report
  • [Presentation] Neospora caninum中間宿主ステージ特異的発色原虫の作出2010

    • Author(s)
      羽多野優芳、池和憲
    • Organizer
      第150回日本獣医学会学術集会
    • Place of Presentation
      帯広畜産大学(北海道)
    • Year and Date
      2010-09-18
    • Related Report
      2010 Annual Research Report
  • [Presentation] Neospora caninumブラディゾイト由来抗原による防御能のマウスモデルにおける検討2010

    • Author(s)
      内田昌樹、池和憲
    • Organizer
      第150回日本獣医学会学術集会
    • Place of Presentation
      帯広畜産大学(北海道)
    • Year and Date
      2010-09-18
    • Related Report
      2010 Annual Research Report
  • [Book] 動物の感染症(第三版)2011

    • Author(s)
      今井壮一, ほか81名
    • Total Pages
      320
    • Publisher
      近代出版
    • Related Report
      2011 Annual Research Report

URL: 

Published: 2010-08-23   Modified: 2019-07-29  

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