Global study using fluorescent Neospora caninum on the vertical transmission mechanism in neosporosis
| Project/Area Number |
22580342
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Basic veterinary science/Basic zootechnical science
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| Research Institution | Nippon Veterinary and Life Science University |
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Principal Investigator |
IKE Kazunori 日本獣医生命科学大学, 獣医学部・獣医学科, 准教授 (50159597)
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| Co-Investigator(Kenkyū-buntansha) |
IMAI Soichi 日本獣医生命科学大学, 獣医学部・獣医学科, 教授 (90120758)
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| Project Period (FY) |
2010 – 2012
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| Project Status |
Completed (Fiscal Year 2012)
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| Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2012: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2011: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2010: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Keywords | 寄生虫 / Neospora caninum / ネオスポラ症 / 垂直感染 / 蛍光発色原虫 / ワクチン |
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| Research Abstract |
At first we visualized Neospora caninum by making a fluorescent color development this protozoan and facilitated various kinds of experiments of neosporosis in this study. We identified the gene encoded the NcBAG1 protein which could become the marker of bradyzoite stage. Furthermore, we confirmed the effect of this protein in the horizontal transmitted mouse model of the antigen derived from bradyzoite. The mixed vaccine of the both antigens showed synergy effects against the horizontal transmission, but the effect was not found in the vertical transmission. We were not able to confirm the effect of the estrogen in the vertical transmission in this study.
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Report
(4 results)
Research Products
(22 results)
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[Journal Article] Comparative study of protective abilities of NcBAG1, NcBSR4, NcMAG1, and NcSAG4 antigens against Neospora caninum infection in acute mouse model.2013
Author(s)
Uchida, M., Nagashima, K., Akatsuka, Y., Ito, A., Imai, S., Ike, K.
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Journal Title
Parasitol. Res.
Volume: 112
Pages: 655-663
Related Report
Peer Reviewed
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[Journal Article] Development 1 of two murine antibodies against Neospora caninum using phage display technology and application on the detection of neosporosis.2013
Author(s)
Dong, J., Otsuki, T., Kato, T., Kohsaka, T., Ike, K., Park, E. Y.
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Journal Title
Related Report
Peer Reviewed
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[Journal Article] Gene cloning and characterization of the protein encoded by the Neospora caninum bradyzoite-specific antigengene BAG1.2013
Author(s)
Kobayashi, T., Narabu, S., Yanai, Y., Hatano, Y., Ito, A., Imai, S., Ike, K.
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Journal Title
J. Parasitol.
Volume: 99
Pages: 453-458
Related Report
Peer Reviewed
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[Journal Article] Detection of Nesopora caninum-specific DNA from cerebrospinal fluid by polymerase chain reaction in a dog with confirmed neosporosis.2012
Author(s)
Ishigaki, K., Noya, M., Kagawa, Y., Ike, K. , Orima, H., Imai, S.
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Journal Title
J. Vet. Med. Sci.
Volume: 74
Pages: 1051-1055
NAID
Related Report
Peer Reviewed
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