Functional analysis of cyclophilins on feline infectious peritonitis virus replication

• Project/Area Number: 22580343
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Basic veterinary science/Basic zootechnical science
• Research Institution: Nippon Veterinary and Life Science University
• Principal Investigator: TANAKA Yoshikazu 日本獣医生命科学大学, 獣医学部, 講師 (50291159)
• Project Period (FY): 2010 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000); Fiscal Year 2012: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2011: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2010: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
• Keywords: ウイルス複製 / サイクロフィリン / ウイルス複製阻害薬 / ウイルス複製機構 / ネコ感染症 / ネコ伝染性腹膜炎 / ウイルス / 感染症

Research Abstract

Feline infectious peritonitis virus (FIPV) causes lethal diseases and no effective vaccine or treatment has been developed. These experiments clarified that Cyclosporin A (CsA) strongly inhibits replication and transcription of FIPV. Also, both cellular cyclophilin A (CypA) and cyclophilin B (CypB), which bind to CsA enhanced the FIPV replication. PPIase activities of Cyp are affected the FIPV replication.

Research Products

• [Journal Article] Suppression of Coronavirus Replication by Cyclophilin Inhibitors. (2013)
  • Author(s): Tanaka Y, Sato Y, Sasaki T
  • Journal Title: Viruses. Volume: 5(5) Issue: 5 Pages: 1250-1260
  • DOI: 10.3390/v5051250
  • Peer Reviewed
• [Journal Article] Feline infectious peritonitis virus witha large deletion in the 5' terminal region of spike gene retains its virulence for cats. (2012)
  • Author(s): Terada Y, Shiozaki Y, Shimoda H, Mahmoud HY, Noguchi K, Nagao Y, Shimojima M, Iwata H, Mizuno T, Okuda M, Morimoto M, Hayashi T, Tanaka Y, Mochizuki M, Maeda K.
  • Journal Title: Journal of General Virology 2012 Volume: 93(Pt 9) Pages: 1930-4
  • Peer Reviewed
• [Journal Article] Population genetic structures of Staphilicoccus aureus isolates from cats and dogs in Japan. (2012)
  • Author(s): Sasaki T, Tsubakishita S, Tanaka Y, Ohtsuka M, Hongo I, Fukata T, Kabeya H, Maruyama S, Hirotaki S, Hiramatsu K.
  • Journal Title: Journal of Clinical Microbiology.
  • Peer Reviewed
• [Journal Article] Suppression of feline coronavirus replication in vitro by cyclosporin A. (2012)
  • Author(s): Tanaka Y, Sato Y, Osawa S, Inoue M, Tanaka S, Sasaki T.
  • Journal Title: Veterinary Research Volume: 43(1) Pages: 41-41
  • Peer Reviewed
• [Journal Article] Suppression of feline corona virus replication in vitro by cyclosporin A (2012)
  • Author(s): Tanaka Y, Sato Y, Osawa S, Inoue M, Tanaka S, Sasaki T.
  • Journal Title: Veterinary Research. Volume: 30:43(1) Issue: 1 Pages: 41-41
  • DOI: 10.1186/1297-9716-43-41
  • Peer Reviewed
• [Journal Article] Feline infectious peritonitis virus with a large deletion in the 5'-terminal region of the spike gene retains its virulence for cats. (2012)
  • Author(s): Terada Y
  • Journal Title: Journal of General Virology Volume: 93(Pt 9): Issue: 9 Pages: 1930-4
  • DOI: 10.1099/vir.0.043992-0
  • Peer Reviewed
• [Journal Article] Population genetic structures of Staphylococcus aureus isolates from cats and dogs in Japan. (2012)
  • Author(s): Sasaki T, Tsubakishita S, Tanaka Y, Ohtsuka M, Hongo I, Fukata T, Kabeya H, Maruyama S, Hiramatsu K.
  • Journal Title: Journal of Clinical Microbiology Volume: 50(6) Issue: 6 Pages: 2152-2155
  • DOI: 10.1128/jcm.06739-11
  • Peer Reviewed
• [Journal Article] Multiplex-PCR method for speciesidentification of coagulase-positive staphylococci. (2010)
  • Author(s): Sasaki T, Tsubakishita S, Tanaka Y, Sakusabe A, Ohtsuka M, Hirotaki S,Kawakami T, Fukata T, Hiramatsu K.
  • Journal Title: Journal of Clinical Microbiology. Volume: 48 (3) Pages: 765-769
  • Peer Reviewed
• [Journal Article] Multiplex-PCR method for species identification of coagulase-positive staphylococci. (2010)
  • Author(s): Sasaki T
  • Journal Title: Journal of Clinical Microbiology Volume: 48 Pages: 765-769
  • Peer Reviewed
• [Presentation] Peptidylprolyl cis-trans isomerase (PPIase) active sites of cyclophilin are crucial for feline coronavirus replication (2013)
  • Author(s): Tanaka Y
  • Organizer: American Society for Virology
  • Place of Presentation: 米国、ペンシルベニア
• [Presentation] ネコ伝染性腹膜炎ウイルスの複製におけるシクロフィリンの役割 (2011)
  • Author(s): 佐藤由佳
  • Organizer: 第152回日本獣医学会学術集会講演要旨集,Page244
  • Place of Presentation: 大阪
• [Presentation] ネコ伝染性腹膜炎ウイルスの複製におけるシクロフィリンの役割 (2011)
  • Author(s): 佐藤由佳
  • Organizer: 第152回日本獣医学会学術集会
  • Place of Presentation: 大阪府立大学・大阪
• [Presentation] ネコ伝染性腹膜炎ウイルス複製におけるシクロフィリン阻害剤の効果 (2010)
  • Author(s): 田中良和
  • Organizer: 第150回 日本獣医学会学術集会
  • Place of Presentation: 帯広畜産大学,北海道
  • Year and Date: 2010-09-16
• [Presentation] Inhibition of feline infectious peritonitis viral replication by cyclosporine. American Society for Virology meeting (2010)
  • Author(s): Yoshikazu Tanaka
  • Organizer: 29th, Montana State University
  • Place of Presentation: Bozeman, MO, USA
  • Year and Date: 2010-07-19
• [Presentation] Inhibition of feline infectious peritonitis viral replication by cyclosporine. (2010)
  • Author(s): Tanaka Y.
  • Organizer: 29^<th> Annual Meeting of American society for virology
  • Place of Presentation: Montana State University USA
  • Year and Date: 2010-07-18
• [Presentation] オリンパス株式会社共催;ランチョンセミナー,予防動物医学の展開 (2010)
  • Author(s): 田中良和、
  • Organizer: 第149回日本獣医学会学術集会講演要旨集
  • Place of Presentation: 東京・日本獣医生命科学大学
  • Year and Date: 2010-03-26
• [Book] 「犬と猫の早期疾病診断学」分担猫のウイルス感染症,予防動物医学研究会編 (2011)
  • Author(s): 田中良和,他
  • Publisher: チクサン出版社
• [Book] 犬と猫の早期疾病診断学 (2011)
  • Author(s): 田中良和
  • Total Pages: 6
  • Publisher: チクサン出版社
• [Patent(Industrial Property Rights)] ネコ伝染性腹膜炎ウイルス(FIPV)の予 (2013)
  • Inventor(s): 田中良和
  • Industrial Property Rights Holder: 学校法人日本医科大学
  • Filing Date: 2013-05-10
• [Patent(Industrial Property Rights)] ネコ伝染性腹膜炎ウイルス(FIPV)の予 (2013)
  • Inventor(s): 田中良和
  • Industrial Property Rights Holder: 学校法人日本医科大学
  • Filing Date: 2013-05-10