Identification of genes involved in Na/K ratio-independent growth of Melissococcus plutonius.
Project/Area Number |
22580345
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Single-year Grants |
Section | 一般 |
Research Field |
Basic veterinary science/Basic zootechnical science
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Research Institution | National Agriculture and Food Research Organization |
Principal Investigator |
TAKAMATSU Daisuke 独立行政法人農業・食品産業技術総合研究機構, 動物衛生研究所細菌・寄生虫研究 領域, 主任研究員 (60414728)
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Co-Investigator(Renkei-kenkyūsha) |
OSAKI Makoto 独立行政法人農業・食品産業技術総合研究機構, 動物衛生研究所細菌・寄生虫研究 領域, 主任研究員 (80355164)
OKURA Masatoshi 独立行政法人農業・食品産業技術総合研究機, 構動物衛生研究所細菌・寄生虫研究 領域, 研究員 (60508315)
|
Project Period (FY) |
2010 – 2012
|
Project Status |
Completed (Fiscal Year 2012)
|
Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2012: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2011: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2010: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
|
Keywords | 病原微生物 / ヨーロッパ腐蛆病菌 / カリウム要求性 / ミツバチ / ヨーロッパ腐蛆病 / 培養性状 / Na+排出ポンプ / 病原細菌の進化 / ゲノム配列 / ナトリウム排出系 / カリウム代謝系 |
Research Abstract |
Melissococcus plutonius is a fastidious honeybee pathogen, and the addition of KH2PO4 to culture medium is required for the growth of typical M. plutonius strains isolated in the world. In contrast, atypical strains isolated only in Japan do not show potassium requirement for the growth. Using genome sequences and a newly developed vector, we showed that mutations in genes encoding Na^+/H^+ antiporter and cation-transporting ATPase are involved in the potassium requirement for the growth of typical M. plutonius strains.
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Report
(5 results)
Research Products
(23 results)
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[Journal Article] Development of Duplex PCR Assay for Detection and Differentiation of Typical and Atypical <i>Melissococcus plutonius</i> strains2014
Author(s)
Rie Arai, Tohru Miyoshi-Akiyama, Kayo Okumura, Yuiko Morinaga, Meihua Wu, Yuya Sugimura, Mikio Yoshiyama, Masatoshi Okura, Teruo Kirikae, Daisuke Takamatsu
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Journal Title
Journal of Veterinary Medical Science
Volume: 76
Issue: 4
Pages: 491-498
DOI
NAID
ISSN
0916-7250, 1347-7439
Related Report
Peer Reviewed / Open Access
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[Journal Article] Diversity of Melissococcus plutonius from Honeybee Larvae in Japan and Experimental Reproduction of European Foulbrood with Cultured Atypical Isolates2012
Author(s)
Rie Arai, Kiyoshi Tominaga, Meihua Wu, Masatoshi Okura, Kazutomo Ito, Naomi Okamura, Hidetaka Onishi, Makoto Osaki, Yuya Sugimura, Mikio Yoshiyama, Daisuke Takamatsu
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Journal Title
Related Report
Peer Reviewed
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[Presentation] Phenotypic and genetic diversity of Melissococcus plutonius isolates from honeybee larvae in Japan and experimental reproduction of European foulbrood by cultured bacteria., ASM20122012
Author(s)
Daisuke Takamatsu, Rie Arai, Kiyoshi Tominaga, Meihua Wu, Masatoshi Okura, Kazutomo Ito, Naomi Okamura, Hidetaka Onishi, Makoto Osaki, Yuya Sugimura,Mikio Yoshiyama
Organizer
112th General Meeting of the American Society for Microbiology
Place of Presentation
Moscone Center, San Francisco (USA)
Related Report
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[Presentation] Phenotypic and genetic diversity of Melissococcus plutonius isolates from honeybee larvae in Japan and experimental reproduction of European foulbrood by cultured bacteria2012
Author(s)
Daisuke Takamatsu, Rie Arai, Kiyoshi Tominaga, Meihua Wu, Masatoshi Okura, Kazutomo Ito, Naomi Okamura, Hidetaka Onishi, Makoto Osaki, Yuya Sugimura, Mikio Yoshiyama
Organizer
ASM2012, 112th General Meeting of the American Society for Microbiology
Place of Presentation
Moscone Center, San Francisco, USA
Related Report
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[Presentation] Diversity of Melissococcus plutonius Isolates from Honeybee Larvae in Japan2012
Author(s)
Daisuke Takamatsu, Rie Arai, Kiyoshi Tominaga, Meihua Wu, Masatoshi Okura, Kazutomo Ito, Naomi Okamura, Hidetaka Onishi, Makoto Osaki, Yuya Sugimura, Mikio Yoshiyama
Organizer
Thailand-Japan Joint Conference on Animal Health 2012: The 25th Year Anniversary of National Institute of Animal Health
Place of Presentation
Rama Gardens Hotel, Bangkok, Thailand
Related Report
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