| Budget Amount *help |
¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000)
Fiscal Year 2012: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2011: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2010: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
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| Research Abstract |
Black flies are blood sucking insects, and transmit a filarial parasite which cause zoonotic onchocerciasis and Leucocytozoon lavati of an endangered rock ptarmigan in Japan. But similarity in morphological characters of adult female made difficult to identify some species. To identify black fly species based on DNA sequences, sequences of mitochondrial genes (CO1, CO2 and 16S rRNA) were determined for 50 black fly species of Japan. So me species had similar sequences in 16S rRNA. But in CO1 and CO2 regions, all species have divergent sequences enough for species identification.
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