• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Osteoinductivity of gelatin/β-tricalcium phosphate sponges loaded with different concentrations of mesenchymal stem cells and bone morphogenetic protein-2 in an equine bone defect.

Research Project

Project/Area Number 22580360
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Clinical veterinary science
Research InstitutionObihiro University of Agriculture and Veterinary Medicine

Principal Investigator

SASAKI Naoki  帯広畜産大学, 畜産学部, 准教授 (00360975)

Project Period (FY) 2010 – 2012
Project Status Completed (Fiscal Year 2012)
Budget Amount *help
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2012: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2011: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2010: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Keywords再生医療 / 骨 / 関節軟骨 / ゼラチンハイドロゲル / 骨形成蛋白 / 多血小板血漿 / 生体組織工学 / 足場 / ドラックデリバリーシステム / 幹細胞 / ウマ / 骨折 / 軟骨 / 骨スクリュー / スキヤフォールド
Research Abstract

β-TCP sponge was an excellent scaffold material, which builds a DDS for BMP-2. Based on these results, it was shown that the combined use of growth factors and scaffolds is effective in bone regeneration. it was suggested that the combined use of MSC, BMP-2 and □-TCP sponge efficiently promoted bone regeneration. Based on the results of the five studies, it was demonstrated that the application of tissue engineering combining growth factors, cells, and scaffolds promoted bone and articular cartilage regeneration. Therefore, future clinical applications to bone and articular cartilage diseases of horses are expected.

Report

(4 results)
  • 2012 Annual Research Report   Final Research Report ( PDF )
  • 2011 Annual Research Report
  • 2010 Annual Research Report
  • Research Products

    (26 results)

All 2013 2012 2011 2010

All Journal Article (12 results) (of which Peer Reviewed: 4 results) Presentation (14 results)

  • [Journal Article] Use of a bioengineered osteochondral precursor for treatment of osteochondritis dissecans in a Thoroughbred filly2013

    • Author(s)
      Nao Tsuzuki, Jong-pil Seo, Shingo Haneda, Kazutaka Yamada, Yasuhiko Tabata, Naoki Sasaki
    • Journal Title

      Australian Veterinary Journal

      Volume: (in press)

    • Related Report
      2012 Final Research Report
  • [Journal Article] Comparison of allogeneic platelet lysate and fetal bovine serum for in vitro expansion of equine bone marrow-derived mesenchymal stem cells2013

    • Author(s)
      Jong-pil Seo, Nao Tsuzuki, Shingo Haneda, Kazutaka Yamada, Hidefumi Furuoka, Yasuhiko Tabata, Naoki Sasaki
    • Journal Title

      Research in Veterinary Science

      Volume: (in press)

    • Related Report
      2012 Final Research Report
  • [Journal Article] The effect of bone morphogenetic protein-2, platelet-rich-plasma incorporated gelatin hydrogel microsphere, mesenchymal stem cell and gelatin s-tricalcium phosphate sponge on equine articular cartilage defect2013

    • Author(s)
      Nao Tsuzuki, Jong-pil Seo, Kazutaka Yamada, Shingo Haneda, Hidefumi Furuoka, Yasuhiko Tabata, Naoki Sasaki
    • Journal Title

      Canadian Veterinary Journal

      Volume: (in print)

    • Related Report
      2012 Final Research Report
  • [Journal Article] The effect of gelatin hydrogel sheet containing bFGF on proximal sesamoid bone transverse fracture in the horse2013

    • Author(s)
      Naoki Sasaki, Satoru Nishii, Kazutaka Yamada, Hidehumi Huruoka, Yasuhiko Tabata
    • Journal Title

      Journal of Euine Veterinary Science

      Volume: 33・17 Pages: 210-214

    • Related Report
      2012 Final Research Report
  • [Journal Article] The effect of gelatin hydrogel sheet containing bFGF on proximal sesamoid bone transverse fracture in the horse.2013

    • Author(s)
      Naoki Sasaki, Satoru Nishii, Kazutaka Yamada, Hidehumi Huruoka, Yasuhiko Tabata
    • Journal Title

      Journal of Euine Veterinary Science

      Volume: 33 Pages: 210214-210214

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] The effect of bone morphogenetic protein-2, platelet-rich-plasma incorporated gelatin hydrogel microsphere, mesenchymal stem cell and gelatin ß-tricalcium phosphate sponge on equine articular cartilage defect.2013

    • Author(s)
      Nao Tsuzuki, Jong-pil Seo, Kazutaka Yamada, Shingo Haneda, Hidefumi Furuoka, Yasuhiko Tabata, Naoki Sasaki
    • Journal Title

      Canadian Veterinary Journal

      Volume: 1 Pages: 35-35

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Comparison of allogeneic platelet lysate and fetal bovine serum for in vitro expansion of equine bone marrow-derived mesenchymal stem cells.2013

    • Author(s)
      Jong-pil Seo, Nao Tsuzuki, Shingo Haneda, Kazutaka Yamada, Hidefumi Furuoka, Yasuhiko Tabata, Naoki Sasaki
    • Journal Title

      Research in Veterinary Science

      Volume: 2 Pages: 15-15

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Effect of compound of gelatin hydrogel microsphere incorporated with platelet-rich-plasma and alginate on sole defect in cattle2012

    • Author(s)
      Nao Tsuzuki, Jong-pil Seo, Kazutaka Yamada, Shingo Haneda, Yasuhiko Tabata and Naoki Sasaki
    • Journal Title

      Journal of Veterinary Medical Science

      Volume: 74 ・8 Pages: 1041-1044

    • NAID

      130001879793

    • Related Report
      2012 Final Research Report
  • [Journal Article] Proliferation of equine bone marrow-derived mesenchymal stem cells in gelatin/β-tricalcium phosphate sponges2012

    • Author(s)
      Jong-pil Seo, Nao Tsuzuki, Shingo Haneda, Kazutaka Yamada, Hidehumi Furuoka, Yasuhiko Tabata, Naoki Sasaki
    • Journal Title

      Research in Veterinary Science

      Volume: 93 ・16 Pages: 1481-1486

    • Related Report
      2012 Annual Research Report 2012 Final Research Report
  • [Journal Article] In vivo osteoinductivity of gelatin β-tri-calcium phosphate sponge and bone morphogenetic protein-2 on an equine third metacarpal bone defect2012

    • Author(s)
      N. Tsuzuki, K. Otsuka, J. Seo, K. Yamada, S. Haneda, H. Furuoka, Y. Tabata, N. Sasaki
    • Journal Title

      Research in Veterinary Science

      Volume: 93・15 Pages: 1021-1025

    • Related Report
      2012 Annual Research Report 2012 Final Research Report
  • [Journal Article] 骨関節疾患に関する再生医療研究の目指すもの2011

    • Author(s)
      佐々木直樹
    • Journal Title

      北海道獣医師会雑誌

      Volume: 55 Pages: 545-549

    • Related Report
      2011 Annual Research Report
  • [Journal Article] basic Fibroblast Growth Factor含浸ゼラチンハイドロゲルシートならびにバンコマイシン含有ポリ乳酸グルコール酸共重合体シートを用いた中足骨開放骨折の乳用子牛の1症例2010

    • Author(s)
      瀬尾洋行、佐々木直樹、杉山仁志、都築直、西井知、大塚健史、山田明夫、田畑泰彦
    • Journal Title

      日本獣医師会雑誌

      Volume: 63巻 Pages: 435-438

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Presentation] 馬の距骨外側滑車の関節軟骨欠損に対する生体組織工学を用いた2層法の影響2012

    • Author(s)
      田邊貴史、都築直、徐鐘筆、羽田真悟、古岡秀文、山田一孝、田畑泰彦、佐々木直樹
    • Organizer
      第25回日本ウマ科学会学術集会
    • Year and Date
      2012-12-03
    • Related Report
      2012 Final Research Report
  • [Presentation] ウマの骨および関節軟骨に対する生体組織工学を用いた再生医療の応用2012

    • Author(s)
      都築直、徐鐘筆、大塚健史、中尾奨吾、大下のえ、山田一孝、羽田真悟、古岡田畑泰彦、佐々木直樹
    • Organizer
      第25回日本ウマ科学会学術集会
    • Year and Date
      2012-12-03
    • Related Report
      2012 Final Research Report
  • [Presentation] The effect of bone morphogenetic protein-2, gelatin hydrogel microsphere incorporating platelet rich plasma, bone marrow derived mesenchymal stem cell and gelatin b-tri calcium phosphate sponge on an equine cartilage defect2012

    • Author(s)
      Nao Tsuzuki, Jong-pil Seo, Kazutaka Yamada, Shingo Haneda, Hidefumi Furuoka, Yasuhiko Tabata, Naoki Sasaki
    • Organizer
      2012 American college of veterinary surgeons veterinary symposium (USA)
    • Year and Date
      2012-11-01
    • Related Report
      2012 Final Research Report
  • [Presentation] Proliferation of equine bone marrow-derived mesenchymal stem cells in gelatin/β-tricalcium phosphate sponges2012

    • Author(s)
      Jong-pil Seo, Nao Tsuzuki, Shingo Haneda, Kazutaka Yamada, Hidehumi Furuoka1, Yasuhiko Tabata, Naoki Sasaki
    • Organizer
      2012 American college of veterinary surgeons veterinary symposium (USA)
    • Year and Date
      2012-11-01
    • Related Report
      2012 Final Research Report
  • [Presentation] 足場内における馬幹細胞増殖に関するin vitro での検討2011

    • Author(s)
      徐鐘筆、都築直、中尾奨吾、大下のえ、田畑泰彦、羽田真悟、山田一孝、佐々木直樹
    • Organizer
      日本ウマ科学会
    • Year and Date
      2011-11-28
    • Related Report
      2012 Final Research Report
  • [Presentation] ウマの関節軟骨欠損モデルに対する多血小板血漿ならびに鏡視下ドリリングの影響2011

    • Author(s)
      大下のえ、都築直、徐鐘筆、中尾奨吾、古岡秀文、田畑泰彦、山田一孝、羽田真悟、佐々木直樹
    • Organizer
      日本ウマ科学会
    • Year and Date
      2011-11-28
    • Related Report
      2012 Final Research Report 2011 Annual Research Report
  • [Presentation] ウマの骨欠損モデルに対する間葉系幹細胞混合BMP-2含浸ゼラチンβ-TCPスポンジの影響2011

    • Author(s)
      中尾奨吾、都築直、徐鐘筆、大下のえ、田邊貴文、山家史、古岡秀文、田畑泰彦、山田一孝、羽田真悟、佐々木直樹
    • Organizer
      日本ウマ科学会
    • Year and Date
      2011-11-28
    • Related Report
      2012 Final Research Report 2011 Annual Research Report
  • [Presentation] ウマの離断性骨軟骨症に対して骨髄由来間葉系幹細胞、BMP-2ならびに多血小板血漿含浸ゼラチンβ-TCPスポンジを用いた1例2011

    • Author(s)
      都築直、徐鐘筆、大下のえ、中尾奨吾、羽田真悟、山田一孝、古岡秀文、田畑泰彦、佐々木直樹
    • Organizer
      日本ウマ科学会
    • Year and Date
      2011-11-28
    • Related Report
      2012 Final Research Report
  • [Presentation] 足場内における馬幹細胞増殖に関するin vitroでの検討2011

    • Author(s)
      徐鐘筆、都築直、中尾奨吾、大下のえ、田畑泰彦、羽田真悟、山田一孝、佐々木直樹
    • Organizer
      日本ウマ科学会
    • Place of Presentation
      東京大学
    • Year and Date
      2011-11-28
    • Related Report
      2011 Annual Research Report
  • [Presentation] ウマのの離断性骨軟骨症に対して骨髄由来間葉系幹細胞、BMP-2ならびに多血小板血漿含浸ゼラチンβ-TCPスポンジを用いた1例2011

    • Author(s)
      都築直、徐鐘筆、大下のえ、中尾奨吾、羽田真悟、山田一孝、古岡秀文、田畑泰彦、佐々木直樹
    • Organizer
      日本ウマ科学会
    • Place of Presentation
      東京大学
    • Year and Date
      2011-11-28
    • Related Report
      2011 Annual Research Report
  • [Presentation] Effect of gelatin β-tri-calcium phosphate sponge containing bone morphogenetic protein-2 on an equine metacarpal bone defect2011

    • Author(s)
      Sasaki Naoki, Tsuzuki Nao, Otsuka Kenji, Yamada Kazutaka, Haneda Shingo, Furuoka Hidefumi, Tabata Yasukiko
    • Organizer
      2011 Asian Meeting of Animal Medicine Specialties
    • Place of Presentation
      Thailand
    • Year and Date
      2011-05-08
    • Related Report
      2011 Annual Research Report
  • [Presentation] In vivo osteoinductivity of gelatin β-tri-calcium phosphate sponge containing bone morphogenetic protein-2 on an equine third metacarpal bone defect2011

    • Author(s)
      Nao Tsuzuki, Kenji Otsuka, Kazutaka Yamada, Shingo Haneda, Hidehumi Furuoka, Yasuhiko Tabata, Naoki Sasaki
    • Organizer
      2nd Joint Workshop between College of Veterinary Medicine, Seoul National University and United Graduate School of Veterinary Sciences, Gifu University
    • Year and Date
      2011-02-20
    • Related Report
      2012 Final Research Report
  • [Presentation] サラブレッド種ウマにおけるゼラチンβ-リン酸-3-カルシウムスポンジからのBone Morphogenetic Protein-2 徐放化による骨再生効果2010

    • Author(s)
      大塚健史、都築直、中尾奨吾、大下のえ、古岡秀文、羽田真吾、田畑泰彦、山田一孝、佐々木直樹
    • Organizer
      第23回日本ウマ科学会
    • Year and Date
      2010-11-29
    • Related Report
      2012 Final Research Report
  • [Presentation] ウマの第3中手骨骨欠損モデルに対するBone morphogenetic protein-2含浸ゼラチンβ-リン酸-3-カルシウムスポンジの影響2010

    • Author(s)
      大塚健史、都築直、中尾奨吾、大下のえ、古岡秀文、田畑泰彦、山田一孝、佐々木直樹
    • Organizer
      第150回日本獣医学会学術集会
    • Place of Presentation
      帯広畜産大学
    • Year and Date
      2010-09-17
    • Related Report
      2010 Annual Research Report

URL: 

Published: 2010-08-23   Modified: 2019-07-29  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi