Establishment of immunoassays for insulin-like peptide 3 in canine and bovine blood and their application for examination of testicular functions

• Project/Area Number: 22580365
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Clinical veterinary science
• Research Institution: Osaka Prefecture University
• Principal Investigator: KAWATE Noritoshi 大阪府立大学, 大学院・生命環境科学研究科, 准教授 (80221901)
• Co-Investigator(Kenkyū-buntansha): TAKAHASHI Masahiro 大阪府立大学, 大学院・生命環境科学研究科, 助教 (50582334) ; TAMADA Hiromichi 大阪府立大学, 大学院・生命環境科学研究科, 教授 (10155252) ; INABA Toshio 大阪府立大学, 大学院・生命環境科学研究科, 教授 (00137241)
• Project Period (FY): 2010 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000); Fiscal Year 2012: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2011: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2010: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
• Keywords: 臨床繁殖 / 産科 / INSL3 / イヌ / ウシ / 潜在精巣 / テストステロン / ライディッヒ細胞 / EIA法 / TR-FIA法 / 雄イヌ / 雄ウシ / 性成熟過程 / 精巣 / 精巣間質細胞(ライディッヒ細胞) / 酵素免疫測定法(EIA法)

Research Abstract

We established immunoassays for canine and bovine insulin-peptide 3 (INSL3) and examined changes of the hormone secretion during sexual maturation and aging in male animals. We also analyzed the INSL3 secretion in cryptorchid cases to examine pathological condition and application to its diagnosis. As the results, the change of INSL3 concentration differed from those of testosterone during puberty. It is elucidated that endocrine function of testicular Leydig cells is reduced in cryptorchid cases. Also, it is suggested that retained testis can be predicted by measuring INSL3 in dogs without testis in scrotum.

Research Products

• [Journal Article] Insulin-like peptide 3 stimulates testosterone secretion in mouse Leydig cells via cAMP pathway (2012)
  • Author(s): Pathirana IN, Kawate N, Bullesbach EE, Takahashi M, Hatoya S, Inaba T, Tamada H.
  • Journal Title: Regul Pept Volume: 178 Issue: 1-3 Pages: 102-6
  • DOI: 10.1016/j.regpep.2012.07.003
  • Peer Reviewed
• [Journal Article] Plasma insulin-like peptide3and testosterone concentrations in male dogs : changes with age and effects of cryptorchidism (2012)
  • Author(s): Pathirana IN, Yamasaki H, Kawate N, Tsuji M, Bullesbach EE, Takahashi M, Hatoya S, Inaba T, Tamada H
  • Journal Title: Theriogenology Volume: 77巻 Issue: 3 Pages: 550-557
  • DOI: 10.1016/j.theriogenology.2011.08.030
  • Peer Reviewed
• [Journal Article] Changes in plasma concentrations of insulin-like peptide 3 and testosterone from birth to pubertal age in beef bulls (2011)
  • Author(s): Kawate N, Ohnari A, Pathirana IN, Sakase M, Bullesbach EE, Takahashi M, Inaba T, Tamada H.
  • Journal Title: Theriogenology. Volume: 76 Issue: 9 Pages: 1632-8
  • DOI: 10.1016/j.theriogenology.2011.07.011
  • Peer Reviewed
• [Journal Article] In vitro effects of estradiol-17β, monobutyl phthalate and mono-(2-ethylhexyl) phthalate on the secretion of testosterone and insulin-like peptide 3 by interstitial cells of scrotal and retained testes in dogs (2011)
  • Author(s): Pathirana IN, Kawate N, Tsuji M, Takahashi M, Hatoya S, Inaba T, Tamada H.
  • Journal Title: Theriogenology. Volume: 76 Pages: 1227-33
  • Peer Reviewed
• [Journal Article] Homozygosity of single nucleotide polymorphisms in the 3' region of the canine estrogen receptor 1 gene is greater in Toy Poodles than in Miniature Dachshunds and Chihuahuas. (2011)
  • Author(s): Pathirana IN, Tanaka K, Kawate N, Tsuji M, Hatoya S, Inaba T, Tamada H.
  • Journal Title: Anim Sci J. Volume: 82 Issue: 3 Pages: 390-5
  • DOI: 10.1111/j.1740-0929.2010.00864.x
  • NAID: 10030376967
  • Peer Reviewed
• [Journal Article] Comparison of testosterone and insulin-like peptide 3 secretions in response to human chorionic gonadotropin in cultured interstitial cells from scrotal and retained testes in dogs (2011)
  • Author(s): Pathirana IN, Ashida Y, Kawate N, Tanaka K, Makoto T, Takahashi M, Hatoya S, Inaba T, Tamada H
  • Journal Title: Anim. Reprod. Sci Volume: 124巻 Issue: 1-2 Pages: 138-144
  • DOI: 10.1016/j.anireprosci.2011.02.014
  • Peer Reviewed
• [Journal Article] Comparison of Plasma Concentrations of Estradiol-17.BETA. and Progesterone, and Conception in Dairy Cows with Cystic Ovarian Diseases between Ovsynch and Ovsynch plus CIDR Timed AI Protocols (2011)
  • Author(s): Kawate N, Watanabe K, Uenaka K, Takahashi M, Inaba T, Tamada H
  • Journal Title: Journal of Reproduction and Development Volume: 57 Issue: 2 Pages: 267-272
  • DOI: 10.1262/jrd.10-066T
  • NAID: 10028275486
  • ISSN: 0916-8818, 1348-4400
  • Peer Reviewed
• [Journal Article] Induction of fertile oestrus in dioestrous bitches by prostaglandin F2α and a GnRH-agonist (2011)
  • Author(s): Ohmura M, Torii R, Hatoya S, Sugiura K, Tamada H, Kawate N, Takahashi M, Sawada T, Inaba T
  • Journal Title: Vet. Rec Volume: 168巻 Issue: 25 Pages: 669-669
  • DOI: 10.1136/vr.d1183
  • Peer Reviewed
• [Journal Article] In vitro effects of estradiol-17β, monobutyl phthalate and mono-(2-ethyl hexyl) phthalate on the secretion of testosterone and insulin-like peptide 3 by interstitial cells of scrotal and retained testes in dogs (2011)
  • Author(s): Pathirana IN, Kawate N, Tsuji M, Takahashi M, Hatoya S, Inaba T, Tamada H
  • Journal Title: Theriogenology Volume: 76巻 Issue: 7 Pages: 1227-1233
  • DOI: 10.1016/j.theriogenology.2011.05.027
  • Peer Reviewed
• [Journal Article] Comparison of testosterone and insulin-like peptide 3 secretions in response to human chorionic gonadotropin in cultured interstitialcells from scrotal and retained testes in dogs (2011)
  • Author(s): Pathirana IN, Ashida Y, Kawate N, Tanaka K, Tsuji M, Takahashi M, Hatoya S, Inaba T, Tamada H.
  • Journal Title: Animal Reproduction Science Volume: 124 Pages: 138-144
  • Peer Reviewed
• [Journal Article] Comparison of Plasma Concentrations of Estradiol-17β and Progesterone, and Conception in Dairy Cows with Cystic Ovarian Diseases between Ovsynch and Ovsynch plus CIDR Timed AI Protocols. (2011)
  • Author(s): Kawate N, Watanabe K, Uenaka K, Takahashi M, Inaba T, Tamada H.
  • Journal Title: Journal of Reproduction and Development Volume: 57(印刷中)
  • NAID: 10028275486
  • Peer Reviewed
• [Journal Article] Homozygosity of single nucleotide polymorphisms in the 3' region of the canine estrogen receptor 1 gene is greater in Toy Poodles than in Miniature Dachshunds and Chihuahuas (2011)
  • Author(s): Pathirana IN, Tanaka K, Kawate N, Tsuji M, Hatoya S, Inaba T, Tamada H.
  • Journal Title: Animal Science Journal Volume: 82(印刷中)
  • NAID: 10030376967
  • Peer Reviewed
• [Journal Article] Analysis of single nucleotide polymorphisms in the 3' region of the estrogen receptor 1 gene in normal and cryptorchid Miniature Dachshunds and Chihuahuas (2010)
  • Author(s): Pathirana IN, Tanaka K, Kawate N, Tsuji M, Kida K, Hatoya S, Inaba T, Tamada H
  • Journal Title: J Reprod Dev Volume: 56 Pages: 405-10
  • NAID: 10026621610
  • Peer Reviewed
• [Journal Article] Analysis of single nucleotide polymorphisms in the 3' region of the estrogen receptor 1 gene in normal and cryptorchid Miniature Dachshunds and Chihuahuas (2010)
  • Author(s): Pathirana IN, Tanaka K, Kawate N, Tsuji M, Kida K, Hatoya S, Inaba T, Tamada H.
  • Journal Title: Journal of Reproduction and Development Volume: 56 Pages: 405-410
  • NAID: 10026621610
  • Peer Reviewed
• [Journal Article] Detection of relaxin mRNA in the corpus luteum, uterus, and uterine cervix in the bitch. (2010)
  • Author(s): Tamada H, Kawate N, Kawata N, Inaba T, Kida K, Hatoya S, Akune A, Nakama K, Kohsaka T, Sawada T.
  • Journal Title: Journal of Veterinary Medical Science Volume: 72 Pages: 1383-1386
  • NAID: 130000264202
  • Peer Reviewed
• [Presentation] Effect of insulin-like peptide 3 on testosterone secretion and cAMP release in mouse Leydig cells. (2013)
  • Author(s): Pathirana IN, Takahashi M, Hatoya S, Inaba T, Tamada H. Kawate N
  • Organizer: Conference on Sri Lanka Japan Collaborative Research 2013, Day 1 Session 6, Health and Hygiene
  • Place of Presentation: ペラデニヤ大学工学部、ペラデニヤ、スリランカ.
  • Year and Date: 2013-03-31
• [Presentation] Effect of Insulin-like Peptide 3 on Testosterone Secretion and cAMP Release in Mouse Leydig Cells (2013)
  • Author(s): Pathirana, I, Takahashi, M, Hatoya, S, Inaba, T, Tamada, H, Kawate, N.
  • Organizer: Conference on Sri Lanka Japan Collaborative Research 2013
  • Place of Presentation: スリランカ ペラデニヤ大学
• [Presentation] Plasma insulin-like peptide 3 and testosterone concentrations in male dogs: hormonal dynamics related to age and cryptorchidism. (2012)
  • Author(s): Pathirana IN, Yamasaki H, Takahashi M, Tamada H, Kawate N.
  • Organizer: Sri Lanka Veterinary Association Annual Meeting, 2012, Common session for selected paper
  • Place of Presentation: 植物遺伝資源センター、ガンノルワ、スリランカ.
  • Year and Date: 2012-05-11
• [Presentation] PLASMA INSULIN-LIKE PEPTIDE 3 AND TESTOSTERONE CONCENTRATIONS IN MALE DOGS: HORMONAL DYNAMICS RELATED TO AGE AND CRYPTORCHIDISM (2012)
  • Author(s): Pathirana, I, Yamasaki, H, Takahashi, M, Tamada, H, Kawate, N.
  • Organizer: Sri Lanka Veterinary Association Annual Meeting 2012
  • Place of Presentation: スリランカ 植物遺伝資源センター
• [Presentation] ウシにおける排卵同期化技術とその応用ならびに精巣内分泌に関する最近の知見 (2011)
  • Author(s): 川手憲俊
  • Organizer: 平成23 年度全国NOSAI 講習会
  • Place of Presentation: NOSAI 兵庫家畜臨床研修所、神戸市.
  • Year and Date: 2011-11-15
• [Presentation] ウシにおける排卵同期化技術とその応用ならびに精巣内分泌に関する最近の知見 (2011)
  • Author(s): 川手憲俊
  • Organizer: 平成23年度全国家畜診療技術講習会
  • Place of Presentation: 兵庫県農業共済組合家畜臨床総合研修所(招待講演)
  • Year and Date: 2011-11-15
• [Presentation] 雄イヌの正常例および潜在精巣例の血中インスリン様ペプチド 3 濃度 (2011)
  • Author(s): 山崎寛子、Indunil Pathirana、Erika Bullesbach、辻 誠、鳩谷晋吾、稲葉俊夫、高橋正弘、玉田尋通、川手憲俊
  • Organizer: 第152回日本獣医学会学術集会 2011, 獣医繁殖分科会 G-9
  • Place of Presentation: 大阪府立大学、堺市.
  • Year and Date: 2011-09-20
• [Presentation] イヌ正常精巣および停留精巣の培養細胞におけるテストステロン分泌に及ぼすインスリン様ペプチド 3 の効果 (2011)
  • Author(s): 藤本泰史、Indunil Pathirana、Erika Bullesbach、辻 誠、鳩谷晋吾、稲葉俊夫、高橋正弘、玉田尋通、川手憲俊
  • Organizer: 第152回日本獣医学会学術集会 2011, 獣医繁殖分科会 G-10
  • Place of Presentation: 大阪府立大学、堺市.
  • Year and Date: 2011-09-20
• [Presentation] Plasma insulin-like peptide 3 and testosterone concentrations in normal and cryptorchid beef bulls (2011)
  • Author(s): Indunil Pathirana、大成衷子、坂瀬充洋、Erika Bullesbach、高橋正弘、稲葉俊夫、玉田尋通、川手憲俊
  • Organizer: 第152回日本獣医学会学術集会 2011, 獣医繁殖分科会 G-11.
  • Place of Presentation: 大阪府立大学、堺市.
  • Year and Date: 2011-09-20
• [Presentation] Plasma insulin-like peptide 3 and testosterone concentrations in normal and cryptorchid beef bulls (2011)
  • Author(s): I.N.Pathirana, A.Ohnari, M.Sakase, E.E.Bullesbach, M.Takahashi, T.Inaba, H.Tamada, N.Kawate
  • Organizer: 第152回獣医学会学術集会
  • Place of Presentation: 大阪府立大学
  • Year and Date: 2011-09-20
• [Presentation] Comparison of secretory response of insulin-peptide 3 and testosterone to hCG in cultured testicular cells of cryptorchid and normal dogs. (2010)
  • Author(s): Indunil Pathirana、芦田ゆきの、田中 翔、辻 誠、鳩谷晋吾、稲葉俊夫、高橋正弘、玉田尋通、川手憲俊
  • Organizer: 第150回日本獣医学会学術集会 2010, 獣医繁殖分科会 G-16
  • Place of Presentation: 帯広畜産大学、帯広市.
  • Year and Date: 2010-09-16
• [Presentation] Comparison of secretory responses of insulin-like peptide 3 and testosterone to hCG in cultured testicular cells of cryptorchid and normal dogs (2010)
  • Author(s): Pathirana IN, Ashida Y, Tanaka K, Tsuji M, Hatoya S, Inaba T, Takahashi M, Tamada H, Kawate N.
  • Organizer: 第150回日本獣医学会学術集会
  • Place of Presentation: 帯広畜産大学
  • Year and Date: 2010-09-16
• [Presentation] Effects of estrogen and phthalates on testosterone secretion in cultured testicular cells from cryptorchid and normal dogs. (2010)
  • Author(s): Pathirana IN, Tanaka K, Tsuji M, Hatoya S, Inaba T, Takahashi M, Tamada H, Kawate N
  • Organizer: 第103回日本繁殖生物学会大会 2010, 臨床応用技術 OR-31
  • Place of Presentation: 北里大学獣医学部、十和田市.
  • Year and Date: 2010-09-04
• [Presentation] Effects of estrogen and phthalates on testosterone secretion in cultured testicular cells from cryptorchid and normal dogs (2010)
  • Author(s): Pathirana IN, Tanaka K, Tsuji M, Hatoya S, Inaba T, Takahashi M, Tamada H, Kawate N.
  • Organizer: 第103回日本生物繁殖学会大会
  • Place of Presentation: 北里大学獣医学部
  • Year and Date: 2010-09-04
• [Book] 獣医繁殖学 (2012)
  • Author(s): 川手憲俊
  • Publisher: 文英堂
• [Remarks]
  • URL: http://www.vet.osakafu-u.ac.jp/reprod/kawate-j.htm
• [Remarks] Noritoshi Kawate, DVM, PhD.
  • URL: http://www.vet.osakafu-u.ac.jp/reprod/kawate-j.htm
• [Remarks]
  • URL: http://www.vet.osakafu-u.ac.jp/reprod/kawate-j.html
• [Remarks]
  • URL: http://www.vet.osakafu-u.ac.jp/reprod/kawate-j.html
• [Patent(Industrial Property Rights)] カテーテル挿入補助器具 (2010)
  • Inventor(s): 川手憲俊
  • Industrial Property Rights Holder: 公立大学大阪府立大学
  • Acquisition Date: 2010-07-28
• [Patent(Industrial Property Rights)] カテーテル挿入補助器具 (2010)
  • Inventor(s): 川手憲俊
  • Industrial Property Rights Holder: 大阪府立大学
  • Filing Date: 2010-05-31
• [Patent(Industrial Property Rights)] カテーテル挿入補助器県 (2010)
  • Inventor(s): 川手憲俊
  • Industrial Property Rights Holder: 大阪府立大学
  • Acquisition Date: 2010-07-28