Establishment of assay system for vaccination adverse reaction and its clinical application.

• Project/Area Number: 22580372
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Clinical veterinary science
• Research Institution: The Institute of Physical and Chemical Research
• Principal Investigator: MASUDA Kenichi 独立行政法人理化学研究所, ワクチンデザイン研究チーム, 客員研究員 (40313077)
• Co-Investigator(Kenkyū-buntansha): SAKAGUCHI Masahiro 麻布大学, 獣医学部, 教授 (20170590)
• Project Period (FY): 2010 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000); Fiscal Year 2012: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000); Fiscal Year 2011: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2010: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
• Keywords: マウス / イヌ / ウシ血清アルブミン / IgE / IgG / ワクチン / 犬 / 牛血清アルブミン / アレルギー / アナフィラキシー

Research Abstract

Recognition site of mouse IgG monoclonal antibody to bovine serum albumin was sequenced for development of recombinant canine IgE against bovine serum albumin. A thresfold of serum IgE concentration to induce in vivo allergic reaction was examined with PK test; however, ideal results were not obtained. It is necessary to select allergens used for this study.

Research Products

• [Journal Article] Large-scale survey of adverse reactions to canine non-rabies combined vaccines in Japan (2012)
  • Author(s): Miyaji, K., Suzuki, A., Shimakura, H., Takase, Y., Kiuchi, A., Fujimura, M., Kurita, G., Tsujimoto, H. and Sakaguchi, M.
  • Journal Title: Veterinary Immunology and Immunopathology Volume: 145 Pages: 447-452
  • Peer Reviewed
• [Journal Article] Flow Cytometric Analysis of Lymphocyte Proliferative Responses to Food Allergens in Dogs with Food Allergy (2011)
  • Author(s): Fujimura, M., et al
  • Journal Title: Journal of Veterinary Medical Science Volume: 73 Issue: 10 Pages: 1309-1317
  • DOI: 10.1292/jvms.10-0410
  • NAID: 130000868205
  • ISSN: 0916-7250, 1347-7439
  • Peer Reviewed
• [Journal Article] Establishment of a quantitative ELISA for the measurement of allergen-specific IgE in dogs using atni-IgE antibody cross-reactive to mouse and dog IgE. (2011)
  • Author(s): Okayama, T., et al.
  • Journal Title: Veterinary Immunology and Immunopathology Volume: 139 Pages: 99-106
  • Peer Reviewed
• [Presentation] 日本における犬混合ワクチン接種後副反応に関する大規模な疫学調査 (2012)
  • Author(s): 宮地一樹、木内明男、藤村正人、栗田吾郎、阪口雅弘
  • Organizer: 第87回麻布獣医学会
  • Place of Presentation: 相模原
  • Year and Date: 2012-11-10
• [Presentation] 犬用ワクチン接種後副反応の対策と最新情報 (2011)
  • Author(s): 藤村正人、蔵田圭吾、阪口雅弘
  • Organizer: 第7回日本獣医内科アカデミー
  • Place of Presentation: 横浜
  • Year and Date: 2011-03-13