| Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2012: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2011: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2010: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
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| Research Abstract |
We have clarified that four chitinases (ChiA, ChiB, ChiC, and ChiD), three β-N-acetylglucosaminidases (GlcNAcases A, B, and C), a transglycosylative enzyme (Hex99), a chitin-binding protein (Cbp1), and two proteases (AprIV and MprIII) are involved in chitin degradation of the marine bacterium, Pseudoalteromonas piscicida strain O-7. Recently, the genome analysis of strain O-7 was performed by Illumina Genome Analyzer. The homology search indicated that there are additional chitinase-, chitin-binding protein-, and N-acetylglucosaminidase-encoding genes in the genome. Real-time quantitative PCR analyses demonstrated that the expression of these genes was induced by the presence of GlcNAc. The proteins were produced in E. coli BL21 (DE3) and purified with Ni Sepharose Fast Flow (GE Healthcare).
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