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Molecular Bases of evolution and degeneration of microbes involved in degradation of environmental pollutants

Research Project

Project/Area Number 22580382
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Boundary agriculture
Research InstitutionBeppu University

Principal Investigator

FURUKAWA Kensuke  別府大学, 食物栄養科学部, 教授 (90221556)

Co-Investigator(Kenkyū-buntansha) HAYASI Takesi  別府大学, 食物栄養科学部, 准教授 (40399811)
藤原 秀彦  別府大学, 食物栄養科学部, 准教授 (10435167)
Project Period (FY) 2010 – 2012
Project Status Completed (Fiscal Year 2012)
Budget Amount *help
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2012: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2011: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2010: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Keywords生物環境 / 環境浄化微生物 / 分解遺伝子 / mer遺伝子 / 水銀耐性菌 / bph-salエレメント / 接合型トランスポゾン / 脱ハロゲン / クロロフォルム / Pseudoal teromonas / Pseudomonas putida / 脱ハロゲン細菌 / Desulfitobacterium / クロロエテン / PCB分解菌 / クロロエテン脱塩素菌 / pce遺伝子
Research Abstract

We investigated dynamism of genes involved in the degradation of polychlorinated biphenyls (PCBs), organomercurials, and chlorinated ethenes. We revealed that dynamic rearrangements of a conjugative bph-sal element in PCB-degrading bacteria, mer genes in mercury-resistant marine bacteria and dehalogenase genes in organohalide-respiring bacteria, and that various mobile elements were highly involved in the rearrangements.

Report

(4 results)
  • 2012 Annual Research Report   Final Research Report ( PDF )
  • 2011 Annual Research Report
  • 2010 Annual Research Report
  • Research Products

    (45 results)

All 2013 2012 2011 2010 Other

All Journal Article (20 results) (of which Peer Reviewed: 18 results) Presentation (22 results) (of which Invited: 2 results) Book (2 results) Remarks (1 results)

  • [Journal Article] 環境バイオ分解菌の新機能を探る2013

    • Author(s)
      古川謙介
    • Journal Title

      化学と生物

      Volume: 51(1) Pages: 998-1002

    • NAID

      10031146875

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Respiratory chain analysis of Zymomonas mobilis mutants producing high levels of ethanol2012

    • Author(s)
      Hayashi, T., Kato, T. and Furukawa, K
    • Journal Title

      Appl. Environ. Microbiol

      Volume: 78(16) Pages: 5622-5629

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] 呼吸低下株は好気エタノール発酵能と耐熱性を同時に獲得するZymomonas mobilis呼吸欠損株の新しい機能2012

    • Author(s)
      林毅、古川謙介
    • Journal Title

      化学と生物

      Volume: 50(11) Pages: 780-782

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] 環境微生物の新規機能の探索と開発2012

    • Author(s)
      古川謙介
    • Journal Title

      J. Environ. Biotechnol.

      Volume: 12(2) Pages: 97-112

    • Related Report
      2012 Final Research Report
  • [Journal Article] Respiratory chain analysis of Zymomonas mobilis mutants producing2012

    • Author(s)
      Hayashi, T., Kato, T. and Furukawa, K
    • Journal Title

      Appl. Environ. Microbiol.

      Volume: 78 Pages: 5622-5629

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] 環境微生物の新規な代謝機能の探索と開発2012

    • Author(s)
      古川謙介
    • Journal Title

      J. Environ. Biotechnol.

      Volume: 12 Pages: 97-112

    • NAID

      40019971960

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] 呼吸能低下株は好気エタノール発酵能と耐熱性を獲得する Zymomonas2012

    • Author(s)
      林 毅、古川謙介
    • Journal Title

      化学と生物

      Volume: 50 Pages: 780-782

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] 環境バイオ 分解菌の新機能を探る2012

    • Author(s)
      古川謙介
    • Journal Title

      化学と生物

      Volume: 51 Pages: 46-51

    • NAID

      10031146875

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Pseudomonas物語2011

    • Author(s)
      古川謙介
    • Journal Title

      日本生物工学会誌

      Volume: 89 Pages: 549-552

    • NAID

      110008750617

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Putative stress sensors WscA and WscB are involved in hypo-osmotic and acidic pH stress in Aspergillus nidulans.2011

    • Author(s)
      Futagami, T., Nakao, S., Kido, Y., Oka, T., Kajiwara, Y., Takahashi, H., Omori, T., Furukawa, K. and Goto, M.
    • Journal Title

      Eukaryotic Cell

      Volume: 10 Pages: 1504-1515

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Development of a strain for efficient degradation of polychlorinated biphenyls by patchwork assembly of degradation pathways.2011

    • Author(s)
      Ohmori, T., Morita, H., Tanaka, M., Miyauchi, K., Kasai, D., Furukawa, K., Miyashita, K., Ogawa, N, Masai, E. and Fukuda, M.
    • Journal Title

      J. Biosci. Biotechnol.

      Volume: 111(4) Pages: 437-442

    • NAID

      110008607499

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Respiration deficient mutants of Zymomonas mobilis show improved growth and enhanced fermentation under aerobic and high temperature condition.2011

    • Author(s)
      Hayashi, T., Furuta, Y. and Furukawa, K.
    • Journal Title

      J. Biosci. Bioeng.

      Volume: 111(4) Pages: 414-419

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Expression in Esherichia coli of biphenyl 2,3-dioxygenase genes from a gram-positive polychlorinated biphenyl degrader2011

    • Author(s)
      Ohmori, T., Morita, H., Tanaka, M., Tomoi, T., Miyauchi, K., Kasai, D., Furukawa, K., Masai, E. and Fukuda, M.
    • Journal Title

      Rhodococcus jostii RHA1. Biosci. Biotechnol. Biochem.

      Volume: 75(1) Pages: 26-33

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Expression in Escherichia coli of niphenyl 2,3-dioxygenase genes from a gram-positive polychlorinated biphenyl degrader, Rhodococcus jostii RHA12011

    • Author(s)
      Ohmori, T., Morita, H., Tanaka, M., Tomoi, T., Miyauchi, K., Kasai, D., Furukawa, K., Masai, E., Fukuda, M.
    • Journal Title

      Biosci.Biotechnol.Biochem.

      Volume: 75 Pages: 26-33

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Enrichment and characterization of a trichloroethene-dechlorinating consortium containing multiple Dehalococcoides strains2011

    • Author(s)
      Futagami, T., Okamoto, F., Hashimoto, H., Furuzawa K., Higashi, K., Nazir, K.H.M.N.H., Wada, E., Suyama, A., Takegawa, K., Goto, M., Nakamura, K., Furukawa, K.
    • Journal Title

      Biosci.Biotechnol.Biochem.

      Volume: 75 Pages: 1268-1274

    • NAID

      10029328958

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Putative stress sensors WscA and WscB are involved in hypo-osmotic and acidic pH stress in Aspergillus nidulans2011

    • Author(s)
      Futagami, T., Nakao, S., Kido, Y., Oka, T., Kajiwara, Y., Takashita, H., Omori, T., Furukawa, K., Goto, M.
    • Journal Title

      Eukaryotic Cell

      Volume: 10 Pages: 1504-1515

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Pseudomonas物語2011

    • Author(s)
      古川謙介
    • Journal Title

      生物工学会誌

      Volume: 89 Pages: 549-552

    • NAID

      110008750617

    • Related Report
      2011 Annual Research Report
  • [Journal Article] Respiration-deficient mutants of Zymomonas mobilis show improved growth and ethanol fermentation under aerobic and high temperature conditions.2011

    • Author(s)
      Hayashi, T., Furuta, Y., Furukawa, K.
    • Journal Title

      J.Biosci.Bioeng.

      Volume: Jan 12.(Epub ahead of print)

    • NAID

      110008607494

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Hybrid pseudomonads engineered by two step homologous recombination acquire novel degradation abilities toward aromatics and polychlorinated biphenyls.2010

    • Author(s)
      Suenaga, H., Nonaka, H., Fujihara, H., Goto, M. and Furukawa, K.
    • Journal Title

      App. Microbiol.Biotechnol.

      Volume: 88 Pages: 915-923

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Hybrid pseudomonads engineered by two-step homologous recambination acquire novel degradation abilities toward aromatics and polychlorinated biphenyls.2010

    • Author(s)
      Suenaga, H., Nonaka, K., Fujuhara, H., Goto, M., Furukawa, K.
    • Journal Title

      App.Microbiol.Biotechnol.

      Volume: 88 Pages: 915-923

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Presentation] Evolutionary engineering of dioxygenases for degradation of environmental pollutants and for synthesis of novel compounds.2012

    • Author(s)
      古川謙介
    • Organizer
      The 4th International Symposium on Environmental & Renewal Energy (ISERE2012)
    • Place of Presentation
      Pusan, Korea
    • Year and Date
      2012-12-14
    • Related Report
      2012 Final Research Report
  • [Presentation] Zymomonas mobilis高エタノール生産呼吸欠損株はNADH脱水素酵素欠損株である2012

    • Author(s)
      加藤剛士、林毅、古川謙介
    • Organizer
      第19回日本生物工学会九州支部大分大会
    • Place of Presentation
      別府
    • Year and Date
      2012-12-01
    • Related Report
      2012 Final Research Report
  • [Presentation] 自然界から分離した有用醸造公募の特性について2012

    • Author(s)
      新谷大、藤原絵美、丸岡生行、古寺美保子、梶原康博、高下秀春、浅田貴美子、古川謙介
    • Organizer
      第19回日本生物工学会九州支部大分大会
    • Place of Presentation
      別府
    • Year and Date
      2012-12-01
    • Related Report
      2012 Final Research Report
  • [Presentation] 環境微生物の新規な機能探索と開発2012

    • Author(s)
      古川謙介
    • Organizer
      環境バイオテクノロジー学会2012年度大会
    • Place of Presentation
      京都
    • Year and Date
      2012-06-26
    • Related Report
      2012 Final Research Report
  • [Presentation] 高エタノール生産Zymomonas mobilis変異株の呼吸鎖の解析2012

    • Author(s)
      林毅、加藤剛士、古川謙介
    • Organizer
      平成23年度日本農芸化学会大会
    • Place of Presentation
      京都女子大学(京都市)
    • Year and Date
      2012-03-25
    • Related Report
      2011 Annual Research Report
  • [Presentation] 環境中より分離した酵母からのSaccharomyces cerevisiaeの効率的二次スクリーニング法の開発2012

    • Author(s)
      藤原秀彦、古川謙介, 他
    • Organizer
      平成23年度日本農芸化学会大会
    • Place of Presentation
      京都女子大学(京都市)
    • Year and Date
      2012-03-24
    • Related Report
      2011 Annual Research Report
  • [Presentation] 高エタノール生産Zymomonas mobilis変異株の呼吸鎖の解析2012

    • Author(s)
      林毅、加藤剛士、古川謙介
    • Organizer
      平成24年度日本農芸化学会大会
    • Place of Presentation
      京都
    • Year and Date
      2012-03-23
    • Related Report
      2012 Final Research Report
  • [Presentation] 環境中から分離した酵母からのSaccharomyces cerevisiaeの高効率二次スクリーニング法の開発2012

    • Author(s)
      藤原秀彦、古川謙介
    • Organizer
      平成24年度日本農芸化学会大会
    • Place of Presentation
      京都
    • Year and Date
      2012-03-23
    • Related Report
      2012 Final Research Report
  • [Presentation] Ferredoxin及びFerredoxin reductaseをコードするbphA3及びbphA4の融合と融合酵素の機能解析2012

    • Author(s)
      福光裕宣、古川謙介、福田雅夫
    • Organizer
      平成24年度日本農芸化学会大会
    • Place of Presentation
      京都
    • Year and Date
      2012-03-23
    • Related Report
      2012 Final Research Report
  • [Presentation] Ferredoxin及びferredoxin reductaseをコードするbphA3及びbphA4の融合と融合酵素の機能解析2012

    • Author(s)
      福光祐宣、古川謙介、福田雅夫, 他
    • Organizer
      平成23年度日本農芸化学会大会
    • Place of Presentation
      京都女子大学(京都市)
    • Year and Date
      2012-03-23
    • Related Report
      2011 Annual Research Report
  • [Presentation] 変異型ビフェニルジオキシゲナーゼの酵素-基質ドッキング解析2011

    • Author(s)
      児玉崇、廣瀬遵、古川謙介, 他
    • Organizer
      平成23年度日本農芸化学会西日本支部・中四国支部合同大会
    • Place of Presentation
      宮崎大学(宮崎市)
    • Year and Date
      2011-09-16
    • Related Report
      2011 Annual Research Report
  • [Presentation] 変異型ビフェニルジオキシゲナーゼの酵素-基質ドッキング解析2011

    • Author(s)
      児玉崇、廣瀬遵、古川謙介
    • Organizer
      平成23年日本生物工学会西日本支部・中四国支部合同大会
    • Place of Presentation
      宮崎市
    • Year and Date
      2011-09-06
    • Related Report
      2012 Final Research Report
  • [Presentation] 呼吸欠損ザイモモナス菌は好気エタノール発酵能と高温耐性能を獲得する2010

    • Author(s)
      林毅、古川謙介
    • Organizer
      第62回日本生物工学会大会
    • Place of Presentation
      宮崎市
    • Year and Date
      2010-10-29
    • Related Report
      2012 Final Research Report
  • [Presentation] キメラ型ビフェニルジオキシゲナーゼによる安息香酸エチルの変換2010

    • Author(s)
      廣瀬遵、上野雅人、古川謙介
    • Organizer
      第62回日本生物工学会大会
    • Place of Presentation
      宮崎市
    • Year and Date
      2010-10-29
    • Related Report
      2012 Final Research Report
  • [Presentation] 呼吸欠損Zymomonas mobilis RDM変異株の呼吸鎖変異とエタノール生産の関係性に関する研究2010

    • Author(s)
      加藤剛士、林毅、古川謙介
    • Organizer
      平成24年度日本農芸化学会西日本支部および日本栄養・食糧学会九州・沖縄支部合同大会
    • Place of Presentation
      鹿児島
    • Year and Date
      2010-09-29
    • Related Report
      2012 Final Research Report
  • [Presentation] 酸素添加酵素(ジオキシゲナーゼ)の進化工学と分解菌の育種2010

    • Author(s)
      古川謙介
    • Organizer
      酵素・補酵素研究会
    • Place of Presentation
      北九州
    • Year and Date
      2010-09-10
    • Related Report
      2012 Final Research Report
  • [Presentation] 酸素添加酵素(ジオキシゲナーゼ)の進化工学と分解菌の育種2010

    • Author(s)
      古川謙介
    • Organizer
      酵素・補酵素研究会
    • Place of Presentation
      北九州国際会議場
    • Year and Date
      2010-09-10
    • Related Report
      2010 Annual Research Report
  • [Presentation] 環境微生物の新規な機能の探索と開発

    • Author(s)
      古川謙介
    • Organizer
      環境バイオテクノロジー学会大会
    • Place of Presentation
      京都市
    • Related Report
      2012 Annual Research Report
    • Invited
  • [Presentation] 呼吸欠損Zymomonas mobilis RDM変異株の呼吸鎖変異とエタノール生産の関係性に関する研究

    • Author(s)
      加藤剛士、林 毅、古川謙介
    • Organizer
      日本農芸化学会大会西日本支部・日本栄養・食糧学会九州支部合同大会
    • Place of Presentation
      鹿児島市
    • Related Report
      2012 Annual Research Report
  • [Presentation] 自然界から分離した有用醸造酵母の特性について

    • Author(s)
      新谷 大、高下秀春、古川謙介他
    • Organizer
      日本生物工学会九州支部大会
    • Place of Presentation
      別府市
    • Related Report
      2012 Annual Research Report
  • [Presentation] Zymomonas mobilis高エタノール生産呼吸欠損株はNADH脱水素酵素欠損株である

    • Author(s)
      加藤剛士、林 毅、古川謙介
    • Organizer
      日本生物工学会九州支部大会
    • Place of Presentation
      別府市
    • Related Report
      2012 Annual Research Report
  • [Presentation] Evolutionary engineering of dioxygenases for degradation and for synthesis of novel compounds

    • Author(s)
      古川謙介
    • Organizer
      The 4th International Symposium on Environment l & Renewable Energy
    • Place of Presentation
      Pusan, Korea
    • Related Report
      2012 Annual Research Report
    • Invited
  • [Book] Biodegradative Bacteria2013

    • Author(s)
      Futagami, T., Goto, M. and Furukawa, K
    • Publisher
      Springer
    • Related Report
      2012 Final Research Report
  • [Book] Biodegradative Bacteria2013

    • Author(s)
      Futagami, T., Goto, M. and Furukawa, K.
    • Total Pages
      12
    • Publisher
      Springer
    • Related Report
      2012 Annual Research Report
  • [Remarks]

    • URL

      http://www.beppu-u.ac.jp/course/nutrition/ferment/

    • Related Report
      2012 Final Research Report

URL: 

Published: 2010-08-23   Modified: 2019-07-29  

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