Research Project
Grant-in-Aid for Scientific Research (C)
Poly(A)-binding protein, which binds poly(A) tail of eukaryotic mRNAs, is a central player of mRNA metabolism, including mRNA stability, translation, and decay. We have previously shown that poly(A)-binding proteins, PABPC1 and PABPC2, interact with a testis-specific Argonaute protein, MIWI/PIWIL1, suggesting that MIWI is involved in translational regulation during spermatogenesis. Contrary to our expectation, MIWI had no significant effects on translation, when tethered to reporter mRNAs. Moreover, MIWI was barely detected in translationally inactive mRNP fractions. These results suggest that MIWI is not implicated in, at least, translational repression. We also elucidated the functionof PABPC2, a testis-specific PABPC, using knock-out mice.
All 2013 2012 2011 2010 Other
All Journal Article (3 results) (of which Peer Reviewed: 3 results) Presentation (22 results) Remarks (4 results)
Biol. Reprod
Volume: 88 Issue: 4 Pages: 1-8
10.1095/biolreprod.112.107425
Biochim Biophys Acta.
Volume: 1804(6) Issue: 6 Pages: 1272-1284
10.1016/j.bbapap.2010.02.004
Biochimica et Biophysica Acta
Volume: 1804 Pages: 1272-1284
http://www.acroman.org/
