Metabolic regulation of amino acid citrulline by nitrogen sensor protein P-II

• Project/Area Number: 22580385
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Applied molecular and cellular biology
• Research Institution: Tottori University (2012); Nara Institute of Science and Technology (2010-2011)
• Principal Investigator: AKASHI Kinya 鳥取大学, 農学部, 准教授 (20314544)
• Project Period (FY): 2010 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000); Fiscal Year 2012: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2011: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2010: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
• Keywords: シトルリン / 抗酸化性 / 窒素代謝 / P-IIタンパク質 / アミノ酸 / 脂質代謝 / 光合成代謝 / センサータンパク質 / P-II / 野生種スイカ

Research Abstract

Wild watermelon is a drought-resistant plant and accumulates antioxidative amino acid citrulline under stress condition. It is revealed that feedback regulation of AGK by arginine is relaxed by the nitrogen sensor P-II protein. Massive degradation of photosynthetic proteins is enhanced under stress, which serves as the nitrogen source for citrulline biosynthesis. Stress-induced proteases are involved in this degradation process.

Research Products

• [Journal Article] Potential involvement of N-terminal acetylation in the quantitative regulation of the ε subunit of chloroplast ATP synthase under drought stress (2013)
  • Author(s): Hoshiyasu, S., Kohzuma, K., Yoshida, K., Fujiwara, M., Fukao, Y., Yokota, A., Akashi, K.
  • Journal Title: Biosci. Biotechnol. Biochem.
  • NAID: 10031177588
• [Journal Article] Potential involvement of N-terminal acetylation in the quantitative regulation of the ε subunit of chloroplast ATP synthase under drought stress. (2013)
  • Author(s): Hoshiyasu S, Kohzuma K, Yoshida K, Fujiwara M, Fukao Y, Yokota A, Akashi K.
  • Journal Title: Biosci. Biotechnol. Biochem. Volume: in print
  • NAID: 10031177588
  • Peer Reviewed
• [Journal Article] Dynamic changes in the leaf proteome of a C3 xerophyte, Citrullus lanatus (wild watermelon) (2011)
  • Author(s): Akashi, K., Yoshida, K., Kuwano, M.,Kajikawa, M., Yoshimura, K.,Hoshiyasu, S., Inagaki, N., Yokota,A
  • Journal Title: in response to water deficit. Planta Volume: 233 Pages: 947-960
• [Journal Article] Responses of the photosynthetic electron transport system to excess light energy caused by water deficit in wild watermelon (2011)
  • Author(s): Sanda, S., Yoshida, K., Kuwano, M.,Kawamura, T., Munekage, Y-N., Akashi,K., Yokota, A
  • Journal Title: Physiol. Plant Volume: 142 Pages: 247-264
• [Journal Article] Responses of the photosynthetic electron transport system to excess light energy caused by water deficit in wild watermelon (2011)
  • Author(s): Sanda, et al
  • Journal Title: Physiol. Plant. Volume: 142 Issue: 3 Pages: 247-264
  • DOI: 10.1111/j.1399-3054.2011.01473.x
  • Peer Reviewed
• [Journal Article] Dynamic changes in the leaf proteome of a C3 xerophyte, Citrullus lanatus (wild watermelon), in response to water deficit. (2011)
  • Author(s): 明石欣也, 他
  • Journal Title: Planta Volume: 233 Pages: 947-960
  • Peer Reviewed
• [Journal Article] Establishment of a transgenic hairy root system in wild and domesticated watermelon (Citrullus lanatus) for studying root vigor under drought (2010)
  • Author(s): Kajikawa, M., Morikawa, K., Abe, Y.,Yokota, A., Akashi, K
  • Journal Title: Plant Cell Rep Volume: 29 Pages: 771-778
• [Presentation] アフリカ原産の野生種スイカ:基礎研究から産業展開へ. 地方独立行政法人鳥取県産業術センター (2012)
  • Author(s): 明石欣也
  • Organizer: 食品開発と健康に関する研究会, 第7回農・畜産物加工分科会
  • Place of Presentation: 倉吉, 鳥取
  • Year and Date: 2012-08-31
• [Presentation] N末端アセチル化修飾による葉緑体ATP合成酵素εサブユニットの量的制御, Involvement of carbon/nitrogen sensor P-II protein in the metabolic adaptation to drought in a xerophyte Citrullus lanatus. Gordon Research Conference on Salt and Drought Stress (2012)
  • Author(s): 星安紗希・吉田和生・藤原正幸・深尾陽一朗・横田明穂・明石欣也・Akashi, K
  • Organizer: 日本農芸化学会本大会, Les Diaberets
  • Place of Presentation: 京都, Switzerland
• [Presentation] Citrullus and Jatropha: Harnessing plant genetic resources for biomass production in Botswana (2011)
  • Author(s): Akashi, K
  • Organizer: 28th IPSR International Symposium (co-sponsored by JSPS-AASPP). "Crop Production in East Africa and Innovative Plant Stress Science"
  • Place of Presentation: Kurashiki, Okayama
  • Year and Date: 2011-10-07
• [Presentation] Citrullus and Jatropha : Harnessing plant genetic resources for biomass production in Botswana (2011)
  • Author(s): Akashi K
  • Organizer: 28th IPSR International Symposium (co-sponsored by JSPS-AASPP). "Crop Production in East Africa and Innovative Plant Stress Science"
  • Place of Presentation: Kurashiki, Okayama
  • Year and Date: 2011-10-07
• [Presentation] 強光・乾燥ストレス下における葉緑体ATP合成酵素εサブユニットの量的制御 (2011)
  • Author(s): 星安紗希・吉田和生・上妻馨梨・藤原正幸・深尾陽一朗・横田明穂・明石欣也
  • Organizer: 日本植物細胞分子生物学会本大会
  • Place of Presentation: 福岡
  • Year and Date: 2011-09-06
• [Presentation] 強光乾燥下の野生種スイカにおけるクチクラ層ワックスの蓄積強化 (2011)
  • Author(s): 明石欣也・渋谷安未・星安紗希・吉田信行・横田明穗
  • Organizer: 日本農芸化学会
  • Place of Presentation: 京都
  • Year and Date: 2011-03-27
• [Presentation] 翻訳後修飾による葉緑体ATP合成酵素εサブユニットの量的制御 (2011)
  • Author(s): 星安紗希・吉田和生・上妻馨梨・深尾陽一朗・横田明穂・明石欣也
  • Organizer: 日本植物生理学会
  • Place of Presentation: 仙台
• [Presentation] 適合溶質シトルリン生合成の鍵酵素N-acetylglutamate kinase の解析 (2010)
  • Author(s): 明石欣也・三輪和哉・高原健太郎・高原(芳野)杏利・横田明 穂
  • Organizer: 日本農芸化学会関西支部大会
  • Place of Presentation: 奈良
  • Year and Date: 2010-10-03
• [Presentation] 適合溶質シトルリン生合成の鍵酵素N-acctylglutamate kinaseの解析 (2010)
  • Author(s): 明石欣也, 他
  • Organizer: 日本農芸化学会関西支部大会
  • Place of Presentation: 生駒市
  • Year and Date: 2010-10-03
• [Presentation] 砂漠植物における適合溶質シトルリンの生合成メカニズム (2010)
  • Author(s): 明石欣也・三輪和哉・高原健太郎・高原(芳野)杏利・横田明 穂
  • Organizer: 日本植物細胞分子生物学会
  • Place of Presentation: 仙台
  • Year and Date: 2010-09-02
• [Presentation] Involvement of post-translational modification in the quantitative regulation of ε subunit of chloroplast ATP synthase in a xerophyte, wild watermelon (2010)
  • Author(s): Hoshiyasu, S., Yoshida, K., Kohzuma, K., Fukao, Y., Yokota, A., Akashi, K
  • Organizer: 15th International Congress of Photosynthesis
  • Place of Presentation: Beijing, China
  • Year and Date: 2010-08-22
• [Presentation] Citrulline metabolism and physiological responses in a drought-tolerant wild watermelon (Citrullus lanatus) inhabiting in the Kalahari Desert (2010)
  • Author(s): Akashi, K
  • Organizer: Africa. Exceptional Microbiological Colloquium. Interfakultares Institut fur Mikrobiologie und infektionsmedizin
  • Place of Presentation: Tubingen University, Tubingen, Germany
  • Year and Date: 2010-06-21
• [Presentation] Citrulline metabolism and physiological responses in a drought-tolerant wild water melon (Citrullus lanatus) inhabiting in the Kalahari Desert Africa. (2010)
  • Author(s): 明石欣也
  • Organizer: Exceptional Microbiological Colloquium Interfakultares Institut fur Mikrobiologie und infektionsmedizin
  • Place of Presentation: Tuebingen, Germany
  • Year and Date: 2010-06-21
• [Presentation] Involvement of carbon/nitrogen sensor P-II protein in the metabolic adaptation to drought in a xerophyte Citrullus lanatus. Gordon Research Conference on Salt and Drought Stress (2010)
  • Author(s): Akashi, K
  • Organizer: Les Diaberets
  • Place of Presentation: Switzerland
  • Year and Date: 2010-06-13
• [Presentation] Involvement of carbon/nitrogen sensor PII protein in the metabolic adaptation to drought in a xerophyte Citrullus lanatus. (2010)
  • Author(s): 明石欣也
  • Organizer: Gordon Research Conference on Salt and Drought Stress
  • Place of Presentation: Les Diaberets, Switzerland
  • Year and Date: 2010-06-13
• [Presentation] アフリカ原産の野生種スイカ：基礎研究から産業展開へ
  • Author(s): 明石欣也
  • Organizer: 地方独立行政法人鳥取県産業術センター, 食品開発と健康に関する研究会
  • Place of Presentation: 倉吉、鳥取
  • Invited
• [Presentation] Exploring genetic resources of drought-resistant wild watermelon from the Kalahari Desert of Africa for the molecular breeding of Jatropha curcas.
  • Author(s): Akashi K.
  • Organizer: Institut Pertanian Bogor Seminar
  • Place of Presentation: Bogor, Indonesia
  • Invited
• [Book] スイカの生理生態と生産事例 (2012)
  • Author(s): 明石欣也、七里吉彦
  • Total Pages: 188
  • Publisher: 誠文堂新光社
• [Book] The Chloroplast Basics and Application (2010)
  • Author(s): 七里吉彦, 他
  • Publisher: Springer
• [Remarks]
  • URL: http://staff.muses.tottori-u.ac.jp/akashikinya/
• [Remarks] 研究紹介、明石欣也
  • URL: http://staff.muses.tottori-u.ac.jp/akashikinya/
• [Remarks]
  • URL: http://bsw3.naist.jp/yokota/Labo_Akashi_Official/Akashi_Kinya.html