| Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2011: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2010: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Research Abstract |
The present investigation was carried out to glycosylate various natural compounds with useful physiological activities by using recombinant glycosyltransferases, and to compare the intestinal absorption of the glycosides. A wide array of plant secondary product glucosyltransferases were cloned from various plant sources and their biochemical functions were characterized. Apocarotenoid glucosyltransferases from Gardenia jasminoides, iridoid-specific glucosyltranferases from Catharanthus roseus, Lonicera japonica and G. jasminoides, and a prenylfuranocoumarin glucosyltransferase from Glehnia littoralis were successfully isolated and functionally characterized, providing a working platform for synthetic biology of plant secondary glucosides. A series of quercetin glycosides were enzymatically synthesized and their intestinal absorption and anti-inflammatory activity were compared.
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