Chemical syntheses of oligosaccharides to elucidate in diagnosis of parasite infection

• Project/Area Number: 22590014
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Chemical pharmacy
• Research Institution: Keio University
• Principal Investigator: HADA Noriyasu 慶應義塾大学, 薬学部, 准教授 (70296531)
• Project Period (FY): 2010 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2012: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2011: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2010: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
• Keywords: 糖鎖合成 / 宿主・寄生相互作用 / 糖脂質 / 糖タンパク質 / 寄生虫 / 糖鎖抗原 / エキノコックス / ブタ回虫 / マンソン住血吸虫 / ビオチンタグ / ELESA / 化学合成 / イヌ回虫 / pseudo Le^Y抗原 / ELISA

Research Abstract

The oligosaccharide residues of glycoprotein and glycosphingolipids in mammalian tissues have been extensively studied for various biological recognition in recent years. In contrast, much less time has been devoted to structures of oligosaccharides from

Research Products

• [Journal Article] Further structural characterization of Echinococcus granulosus laminated layer carbohydrates: The blood-antigen P1-motif gives rise to branches at different points of the O-glycan chains. (2013)
  • Author(s): Gerardo Lin
  • Journal Title: Glycobiology Volume: 23 Issue: 4 Pages: 438-452
  • DOI: 10.1093/glycob/cws220
  • Peer Reviewed
• [Journal Article] Synthetic studies on glycosphingolipids from protostomia phyla:synthesis of glycosphingolipids and related carbohydrate moieties from the parasite Schistosoma mansoni (2012)
  • Author(s): T. Kanaya, F. Schweizer, T. Takeda, F. Kiuchi, N. Hada.
  • Journal Title: Carbohydr. Res. Volume: 361 Pages: 55-72
  • Peer Reviewed
• [Journal Article] Synthesis, antigenicity against human sera and structure-activities relationships of carbohydrate moieties from toxocara larvae and their analogues (2012)
  • Author(s): A. Koizumi, K. Yamano , T. Tsuchiya, F. Schweizer , F. Kiuchi, N. Hada.
  • Journal Title: molecules Volume: 17 Pages: 9023-9042
  • Peer Reviewed
• [Journal Article] Galα1-4Galβ1-3GalNAc is the dominant epitope of Em2 antigen, the mucin-type glycoprotein from Echinococcus multilocularis (2012)
  • Author(s): K. Yamano, A. Koizumi, T. Takeda, F. Kiuchi, N. Hada.
  • Journal Title: Parasitol. Res Volume: 111 Pages: 795-805
  • Peer Reviewed
• [Journal Article] Galα1-4Galβ1-3GalNAc is the dominant epitope of Em2 antigen, the mucin-type glycoprotein from Echinococcus multilocularis (2012)
  • Author(s): Kimiaki Yamano
  • Journal Title: Parasitology Research Volume: 111 Issue: 2 Pages: 795-805
  • DOI: 10.1007/s00436-012-2902-1
  • Peer Reviewed
• [Journal Article] Synthesis, antigenicity against human sera and structure-activities relationships of carbohydrate moieties from toxocara larvae and their analogues (2012)
  • Author(s): Akihiko Koizumi
  • Journal Title: Molecules Volume: 17 Issue: 8 Pages: 9023-9042
  • DOI: 10.3390/molecules17089023
  • Peer Reviewed
• [Journal Article] Synthetic studies on glycosphingolipids from protostomia phyla: synthesis of glycosphingolipids and related carbohydrate moieties from the parasite Schistosoma mansoni (2012)
  • Author(s): Takayuki Kanaya
  • Journal Title: Carbohydrate Research Volume: 361 Pages: 55-72
  • DOI: 10.1016/j.carres.2012.08.008
  • Peer Reviewed
• [Journal Article] Synthesis of the carbohydrate moiety from the parasite Echinococcus multilocularis and their antigenicity against human sera (2011)
  • Author(s): A. Koizumi, K. Yamano , F. Schweizer , T. Takeda, F. Kiuchi, N. Hada.
  • Journal Title: Eur. J. Med. Chem. Volume: 46 Pages: 1768-1778
  • Peer Reviewed
• [Journal Article] Synthesis of the carbohydrate moiety from the parasite Echinococcus multilocularis and their antigenicity against human sera (2011)
  • Author(s): A.Koizumi, K.Yamano, F.Schweizer, T.Takeda, F.Kiuchi, N.Hada.
  • Journal Title: Eur.J.Med.Chem. Volume: 46 Pages: 1768-1778
  • Peer Reviewed
• [Journal Article] Synthetic Studies on Glycosphingolipids from Protostomia phyla : Synthesis of Glycosphingolipids from the Parasite Schistosoma mansoni (2010)
  • Author(s): T.Kanaya, S.Yagi, F.Schweizer, T.Takeda, F.Kiuchi, N.Hada
  • Journal Title: Chem.Pharm.Bull. Volume: 58 Pages: 811-817
  • NAID: 130000255853
  • Peer Reviewed
• [Presentation] 無脊椎動物由来糖脂質の合成研究(51) ブタ回虫 Ascaris suum 由来非還元末端糖鎖の合成(7) (2013)
  • Author(s): 塚本達也、梅田悠奈、羽田紀康、木内文之
  • Organizer: 日本薬学会第133年会
  • Place of Presentation: 横浜
  • Year and Date: 2013-03-29
• [Presentation] 無脊椎動物由来糖脂質の合成研究(51) ブタ回虫 Ascaris suum由来非還元末端側糖鎖の合成(7) (2013)
  • Author(s): 塚本達也
  • Organizer: 日本薬学会第133回年会
  • Place of Presentation: 横浜
• [Presentation] 無脊椎動物由来糖脂質の合成研究(52) 海綿由来の糖脂質 (2013)
  • Author(s): 奥村謙
  • Organizer: 日本薬学会第133回年会
  • Place of Presentation: 横浜
• [Presentation] Echinococcus granulosus由来糖タンパク質の糖鎖部分に関する合成研究 (2012)
  • Author(s): 大鐘麻未、羽田紀康、土屋貴史、中根洋美、小泉晶彦、木内文之、山野公明
  • Organizer: 日本薬学会第132回年会
  • Place of Presentation: 札幌
  • Year and Date: 2012-03-30
• [Presentation] マンソン住血吸虫 Schistosoma mansoni 由来の糖タンパク質糖鎖 (2012)
  • Author(s): 靜間悠志
  • Organizer: 第31回日本糖質学会年会
  • Place of Presentation: 鹿児島
• [Presentation] Echinococcus属由来糖タンパク質の糖鎖部分に関する合成研究(3) (2011)
  • Author(s): 小泉晶彦、大鐘麻未、羽田紀康、木内文之、山野公明
  • Organizer: 第30回日本糖質学会年会
  • Place of Presentation: 長岡
  • Year and Date: 2011-07-13
• [Presentation] イヌ回虫由来糖タンパク質の糖鎖部分に関する合成研究 (2011)
  • Author(s): 羽田紀康、小泉晶彦、土屋貴史、木内文之
  • Organizer: 第30回日本糖質学会年会
  • Place of Presentation: 長岡
  • Year and Date: 2011-07-13
• [Presentation] Echinococcus属由来糖タンパク質の糖鎖部分に関する合成研究(2) (2011)
  • Author(s): 小泉晶彦, 大鐘麻未, 羽田紀康, 木内文之, 山野公明
  • Organizer: 日本薬学会第131年会
  • Place of Presentation: 静岡
  • Year and Date: 2011-03-31
• [Presentation] 無脊椎動物由来糖脂質の合成研究(49)マンソン住血吸虫Schistosoma mansoni由来糖脂質の合成(7) (2011)
  • Author(s): 金谷貴行, 羽田紀康, 竹田忠紘, 木内文之
  • Organizer: 日本薬学会第131年会
  • Place of Presentation: 静岡
  • Year and Date: 2011-03-31
• [Presentation] Synthetic studies on oligosaccharides of glycoprotein from Echinococcus genus (2010)
  • Author(s): Koizumi A, Hada N, Kiuchi F, Yamano K.
  • Organizer: 25th International Carbohydrate Symposium
  • Place of Presentation: 幕張
  • Year and Date: 2010-08-02
• [Presentation] Synthesis of glycosphingolipids and related oligosaccharides from a (human) parasite Schistosoma mansoni (2010)
  • Author(s): Kanaya T, Hada N, Takeda T, Kiuchi F.
  • Organizer: 25th International Carbohydrate Symposium
  • Place of Presentation: 幕張
  • Year and Date: 2010-08-02
• [Presentation] Synthesis of the nonreducing end oligosaccharides of glycosphingolipids from Ascarissuum (2010)
  • Author(s): Kumada H, Shimazaki Y, Hada N, Takeda T, Kiuchi F.
  • Organizer: 25th International Carbohydrate Symposium
  • Place of Presentation: 幕張
  • Year and Date: 2010-08-02
• [Presentation] Echinococcus granulosus 由来糖タンパク質の糖鎖部分に関する合成研究 (2010)
  • Author(s): 大鐘真実、羽田紀康、土屋貴志、中根洋美、小泉明彦、木内文之、山野公明
  • Organizer: 日本薬学会第132 年会
  • Place of Presentation: 札幌
  • Year and Date: 2010-03-30