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Analysis of phosphorylated biomolecules using a phosphate-affinity quartz-crystal microbalance method

Research Project

Project/Area Number 22590037
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Physical pharmacy
Research InstitutionHiroshima University

Principal Investigator

KINOSHITA Eiji  広島大学, 大学院・医歯薬保健学研究院, 准教授 (80304418)

Project Period (FY) 2010 – 2012
Project Status Completed (Fiscal Year 2012)
Budget Amount *help
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2012: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2011: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2010: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
Keywordsリン酸化 / 水晶発振子マイクロバランス / QCM / バイオセンサー / フォスタグ / 分子認識 / 蛋白質 / 分析化学
Research Abstract

In this study, the researcher has applied a novel phosphate capture molecule, biotinylated Phos-tag (Phos-tag Biotin BTL-111), which was originally synthesized, to a quartz-crystal microbalance procedure (QCM). By using the BTL-111,a phosphate-affinity QCM sensor chip has been newly developed. This novel biosensor chip permitted highly sensitive detections of phosphorylated biomolecules, such as phosphoproteins or DNA.

Report

(4 results)
  • 2012 Annual Research Report   Final Research Report ( PDF )
  • 2011 Annual Research Report
  • 2010 Annual Research Report
  • Research Products

    (85 results)

All 2013 2012 2011 2010 Other

All Journal Article (27 results) (of which Peer Reviewed: 27 results) Presentation (44 results) (of which Invited: 1 results) Book (9 results) Remarks (5 results)

  • [Journal Article] Sandwich assay for phosphorylation of protein multiplexes by using antibodies and Phos-tag2013

    • Author(s)
      E. Kinoshita, E. Kinoshita-Kikuta, and T. Koike
    • Journal Title

      Analytical Biochemistry

      Volume: 438 Issue: 2 Pages: 104-106

    • DOI

      10.1016/j.ab.2013.03.029

    • Related Report
      2012 Annual Research Report 2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] A Phos-tag-based magnetic-bead method for rapid and selective separation of phosphorylated biomolecules2013

    • Author(s)
      M. Tsunehiro, Y. Meki, K. Matsuoka, E. Kinoshita-Kikuta, E. Kinoshita and T. Koike
    • Journal Title

      Journal of Chromatography B

      Volume: 925 Pages: 86-94

    • DOI

      10.1016/j.jchromb.2013.02.039

    • Related Report
      2012 Annual Research Report 2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] A Laborsaving, Timesaving, and More Reliable Strategy for Separation of Low-Molecular-Mass Phosphoproteins in Phos-tag Affinity Electrophoresis2012

    • Author(s)
      E. Kinoshita-Kikuta, E. Kinoshita, and T. Koike
    • Journal Title

      International Journal of Chemistry

      Volume: 4 Issue: 5 Pages: 1-8

    • DOI

      10.5539/ijc.v4n5p1

    • Related Report
      2012 Annual Research Report 2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] EF-1α and mitochondrial ATP synthase ・ chain: Alteration of their expression in encystment-induced Colpoda cucullus2012

    • Author(s)
      Sogame, Y., Kojima, K., Takeshita, T.,Eiji Kinoshita, and Matsuoka, T
    • Journal Title

      J. Eukaryot. Microbiol

      Volume: 59 Issue: 4 Pages: 401-406

    • DOI

      10.1111/j.1550-7408.2012.00628.x

    • Related Report
      2012 Annual Research Report 2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] リン酸化プロテオミクスのための Phos-tag テクノロジー2012

    • Author(s)
      木下英司,木下恵美子,小池透
    • Journal Title

      分析化学

      Volume: 61 Pages: 469-487

    • NAID

      10030436756

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Protein phosphorylation in encystment-induced Colpoda cucullus: localization and identification of phosphoproteins.2012

    • Author(s)
      Sogame, Y.
    • Journal Title

      FEMS Microbiol. Lett.

      Volume: 331 Issue: 2 Pages: 128-135

    • DOI

      10.1111/j.1574-6968.2012.02560.x

    • Related Report
      2012 Annual Research Report 2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Highly sensitive detection of protein phosphorylation by using improved Phos-tag Biotin2012

    • Author(s)
      E. Kinoshita, E. Kinoshita-Kikuta, Y. Sugiyama, Y. Fukada, T. Ozeki, and T. Koike
    • Journal Title

      Proteomics

      Volume: 12 Issue: 7 Pages: 932-937

    • DOI

      10.1002/pmic.201100639

    • Related Report
      2012 Annual Research Report 2012 Final Research Report 2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Separation and identification of four distinct serine-phosphorylation states of ovalbumin by Phos-tag affinity electrophoresis2012

    • Author(s)
      E. Kinoshita-Kikuta, E. Kinoshita, and T. Koike
    • Journal Title

      Electrophoresis

      Volume: 33 Issue: 5 Pages: 849-855

    • DOI

      10.1002/elps.201100518

    • Related Report
      2012 Final Research Report 2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] プロトコール集 フォスタグテクノロジーを用いたリン酸化タンパク質解析法2012

    • Author(s)
      木下恵美子,木下英司,小池透
    • Journal Title

      生物物理化学

      Volume: 56

    • NAID

      130004809457

    • URL

      https://www.jstage.jst.go.jp/browse/sbk/56/Suppl_2/_contents/-char/ja/

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] 今後の展望2 蛍光性Phos-tag を用いたタンパク質リン酸化反応の解析法2012

    • Author(s)
      木下英司,木下恵美子,多幾山敬,宗村雅男,小池透
    • Journal Title

      生物物理化学

      Volume: 56

    • NAID

      130004809456

    • URL

      https://www.jstage.jst.go.jp/browse/sbk/56/Suppl_2/_contents/-char/ja/

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] 今後の展望1 中性条件で泳動を行う改良型 Phos-tag SDS-PAGE を用いたリン酸化タンパク質解析2012

    • Author(s)
      木下恵美子,木下英司,小池透
    • Journal Title

      生物物理化学

      Volume: 56

    • NAID

      130004809455

    • URL

      https://www.jstage.jst.go.jp/browse/sbk/56/Suppl_2/_contents/-char/ja/

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] フォスタグケミストリー2012

    • Author(s)
      木下英司,木下恵美子,小池透
    • Journal Title

      生物物理化学

      Volume: 56

    • NAID

      130004809452

    • URL

      https://www.jstage.jst.go.jp/browse/sbk/56/Suppl_2/_contents/-char/ja/

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Phos-tag SDS-PAGE systems for phosphorylation profiling of proteins with a wide range of molecular masses under neutral pH conditions2012

    • Author(s)
      E. Kinoshita, E. Kinoshita-Kikuta, and T. Koike
    • Journal Title

      Proteomics

      Volume: 12 Issue: 2 Pages: 192-202

    • DOI

      10.1002/pmic.201100524

    • Related Report
      2012 Final Research Report 2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] フォスタグケミストリー2012

    • Author(s)
      木下英司
    • Journal Title

      生物物理化学

      Volume: 56

    • NAID

      130004809452

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] 今後の展望1中性条件で泳動を行う改良型Phos-tag SDS-PAGEを用いたリン酸化タンパク質解析2012

    • Author(s)
      木下恵美子
    • Journal Title

      生物物理化学

      Volume: 56

    • NAID

      130004809455

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] 今後の展望2蛍光性Phos-tagを用いたタンパク質リン酸化反応の解析法2012

    • Author(s)
      木下英司
    • Journal Title

      生物物理化学

      Volume: 56

    • NAID

      130004809456

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] プロトコール集フォスタグテクノロジーを用いたリン酸化タンパク質解析法2012

    • Author(s)
      木下恵美子
    • Journal Title

      生物物理化学

      Volume: 56

    • NAID

      130004809457

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Possible involvement of cAMP and protein phosphorylation in the cell signaling pathway for resting cyst formation of ciliated protozoan Colpoda cucullus2011

    • Author(s)
      Sogame, Y., Asami, H., Eiji Kinoshita, and Matsuoka, T
    • Journal Title

      Acta Protozool

      Volume: 50 Pages: 71-79

    • URL

      http://www.eko.uj.edu.pl/ap/index.php?option=com_docman&task=cat_view&gid=42&Itemid=27

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Ca2+-dependent in vivo protein phosphorylation and encystment induction in the ciliated protozoan Colpoda cucullus2011

    • Author(s)
      Sogame, Y., Eiji Kinoshita, and Matsuoka, T
    • Journal Title

      Eur. J. Protistol

      Volume: 4 7 Issue: 3 Pages: 208-213

    • DOI

      10.1016/j.ejop.2011.02.003

    • Related Report
      2012 Final Research Report 2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] A Phos-tag-based fluorescence resonance energy transfer system for the analysis of the kinase reaction of a substrate peptide2011

    • Author(s)
      M. Somura, K. Takiyama, E. Kinoshita- Kikuta, E. Kinoshita, and T. Koike
    • Journal Title

      Analytical Methods

      Volume: 3 Issue: 6 Pages: 1303-1309

    • DOI

      10.1039/c1ay05016h

    • Related Report
      2012 Final Research Report 2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] A novel phosphate-affinity bead with immobilized Phos-tag for separation and enrichment of phosphopeptides and phosphoproteins2011

    • Author(s)
      Kinoshita-Kikuta, E., Yamada, A., Inoue, C., Eiji Kinoshita, and Koike, T
    • Journal Title

      J. Integrated OMICS

      Volume: 1 Issue: 1 Pages: 157-169

    • DOI

      10.5584/jiomics.v1i1.46

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Improved Phos-tag SDS-PAGE under neutral pH conditions for advanced protein phosphorylation profiling2011

    • Author(s)
      Eiji Kinoshita and Kinoshita-Kikuta, E
    • Journal Title

      Proteomics

      Volume: 11 Issue: 2 Pages: 319-323

    • DOI

      10.1002/pmic.201000472

    • NAID

      130005454290

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Zinc(II)-cyclen polyacrylamide gel electrophoresis for detection of mutations in short Ade/Thy-rich DNA fragments2011

    • Author(s)
      Kinoshita-Kikuta, E., Eiji Kinoshita, Harada, N., and Koike, T
    • Journal Title

      Anal. Biochem

      Volume: 408 Issue: 2 Pages: 348-350

    • DOI

      10.1016/j.ab.2010.09.003

    • NAID

      120002753732

    • URL

      http://ir.lib.hiroshima-u.ac.jp/00030873

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Possible involvement of cAMP and protein phosphorylation in the cell signaling pathway for resting cyst formation of ciliated protozoan Colpoda cucullus2011

    • Author(s)
      Sogame, Y.
    • Journal Title

      Acta Protozool.

      Volume: 50 Pages: 71-79

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Zinc(II)-cyclen polyacrylamide gel electrophoresis for detection of mutations in short Ade/Thy-rich DNA fragments.2011

    • Author(s)
      Kinoshita-Kikuta, E.
    • Journal Title

      Anal.Biochem.

      Volume: 408 Pages: 348-350

    • NAID

      120002753732

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Improved Phos-tag SDS-PAGE under neutral pH conditions for advanced protein phosphorylation profiling.2011

    • Author(s)
      Kinoshita, E.
    • Journal Title

      Proteomics

      Volume: 11 Pages: 319-323

    • NAID

      130005454290

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] A novel phosphate-affinity bead with immobilized Phos-tag for separation and enrichment of phosphopeptides and phosphoproteins.2011

    • Author(s)
      Kinoshita-Kikuta, E.
    • Journal Title

      J.Integr.Omics

      Volume: 1 Pages: 157-169

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Presentation] Highly sensitive imaging of protein phosphorylation by Phos-tag technology2013

    • Author(s)
      Eiji Kinoshita
    • Organizer
      1st International Symposium on Protein Modifications in Pathogenic Dysregulation of Signaling
    • Place of Presentation
      東大医科研講堂,東京
    • Year and Date
      2013-02-01
    • Related Report
      2012 Final Research Report
  • [Presentation] Phos-tag 技術が拓くタンパク質リン酸化研究の新たな潮流2012

    • Author(s)
      木下英司
    • Organizer
      第85回日本生化学会大会
    • Place of Presentation
      マリンメッセ福岡,福岡市
    • Year and Date
      2012-12-16
    • Related Report
      2012 Final Research Report
  • [Presentation] SuperSep Phos-tag プレキャストゲル:迅速,高効率,かつ,高精度なリン酸化シグナル伝達分子の分離検出法2012

    • Author(s)
      木下英司,木下恵美子,小池透
    • Organizer
      第35回日本分子生物学会年会
    • Place of Presentation
      マリンメッセ福岡,福岡市
    • Year and Date
      2012-12-11
    • Related Report
      2012 Final Research Report
  • [Presentation] Phos-tag 技術によって新たにできるようになったこと2012

    • Author(s)
      木下英司
    • Organizer
      新薬理学セミナー 2012
    • Place of Presentation
      熊本大学薬学部宮本記念館,熊本市(招待講演)
    • Year and Date
      2012-11-24
    • Related Report
      2012 Final Research Report
  • [Presentation] Phos-tag SDS-PAGE systems for protein phosphorylation profiling under neutral pH conditions2012

    • Author(s)
      Eiji Kinoshita,Kinoshita-Kikuta, E., and Koike, T
    • Organizer
      HUPO 2012 11th Annual World Congress
    • Place of Presentation
      Hynes Convention Center, Boston, MI, USA
    • Year and Date
      2012-09-12
    • Related Report
      2012 Final Research Report
  • [Presentation] リン酸化タンパク質解析のためのフォスタグテクノロジー2012

    • Author(s)
      木下英司
    • Organizer
      第63回日本電気泳動学会総会
    • Place of Presentation
      沖縄コンベンションセンター,宜野湾市
    • Year and Date
      2012-08-20
    • Related Report
      2012 Final Research Report
  • [Presentation] 改良型Phos-tag Biotin の開発とリン酸化プロテオミクスへの応用2012

    • Author(s)
      木下英司,木下恵美子,小池透
    • Organizer
      日本プロテオーム学会 2012 年大会
    • Place of Presentation
      日本科学未来館,東京
    • Year and Date
      2012-07-26
    • Related Report
      2012 Final Research Report
  • [Presentation] Phos-tag アフィニティ電気泳動の開発と改良~リン酸化タンパク質の分析2012

    • Author(s)
      木下英司
    • Organizer
      第62回日本電気泳動学会シンポジウム
    • Place of Presentation
      慶応義塾大学薬学部,東京
    • Year and Date
      2012-05-11
    • Related Report
      2012 Final Research Report
  • [Presentation] リン酸化生体分子解析のための Phos-tag テクノロジー2012

    • Author(s)
      木下英司
    • Organizer
      第610回 九州大学生医研セミナー
    • Place of Presentation
      九州大学生医研,福岡市(招待講演)
    • Year and Date
      2012-02-23
    • Related Report
      2012 Final Research Report
  • [Presentation] リン酸化生体分子解析のためのPhos-tagテクノロジー2012

    • Author(s)
      木下英司
    • Organizer
      第610回九州大学生医研セミナー
    • Place of Presentation
      博多市(招待講演)
    • Year and Date
      2012-02-23
    • Related Report
      2011 Annual Research Report
  • [Presentation] Phos-tag SDS-PAGE systems for protein phosphorylation profiling under neutral pH conditions2012

    • Author(s)
      Kinoshita, E.
    • Organizer
      HUPO 11th Annual World Congress
    • Place of Presentation
      Hynes Convention Center, Boston, MI, USA
    • Related Report
      2012 Annual Research Report
  • [Presentation] Phos-tag がもたらす蛋白質リン酸化解析のブレークスルー2011

    • Author(s)
      木下英司
    • Organizer
      第34回日本分子生物学会バイオインダストリーセミナー
    • Place of Presentation
      パシフィコ横浜,横浜市(招待講演)
    • Year and Date
      2011-12-13
    • Related Report
      2012 Final Research Report
  • [Presentation] Phos-tagがもたらす蛋白質リン酸化解析のブレークスルー2011

    • Author(s)
      木下英司
    • Organizer
      第34回日本分子生物学会
    • Place of Presentation
      横浜市(招待講演)
    • Year and Date
      2011-12-13
    • Related Report
      2011 Annual Research Report
  • [Presentation] 改良型 Phos-tag Biotin の開発とその応用2011

    • Author(s)
      木下英司
    • Organizer
      第62回日本電気泳動学会
    • Place of Presentation
      横浜市開港記念会館,横浜市
    • Year and Date
      2011-11-13
    • Related Report
      2012 Final Research Report
  • [Presentation] Phos-tag 親和電気泳動法の開発2011

    • Author(s)
      木下英司
    • Organizer
      第62回日本電気泳動学会
    • Place of Presentation
      横浜市開港記念会館,横浜市
    • Year and Date
      2011-11-12
    • Related Report
      2012 Final Research Report
  • [Presentation] Phos-tag親和電気泳動法の開発2011

    • Author(s)
      木下英司
    • Organizer
      第62回日本電気泳動学会(第50回児玉賞受賞講演)
    • Place of Presentation
      横浜市
    • Year and Date
      2011-11-12
    • Related Report
      2011 Annual Research Report
  • [Presentation] 改良型Phos-tag Biotinの開発とその応用2011

    • Author(s)
      木下英司
    • Organizer
      第62回日本電気泳動学会
    • Place of Presentation
      横浜市
    • Year and Date
      2011-11-12
    • Related Report
      2011 Annual Research Report
  • [Presentation] Phos-tag によるタンパク質リン酸化修飾の高感度検出2011

    • Author(s)
      木下英司
    • Organizer
      第5回QCM研究会
    • Place of Presentation
      東京會館,東京都千代田区
    • Year and Date
      2011-10-14
    • Related Report
      2012 Final Research Report
  • [Presentation] Phos-tagによるタンパク質リン酸化修飾の高感度検出2011

    • Author(s)
      木下英司
    • Organizer
      QCM研究会
    • Place of Presentation
      東京都千代田区(招待講演)
    • Year and Date
      2011-10-14
    • Related Report
      2011 Annual Research Report
  • [Presentation] リン酸化プロテオミクスに向けた Phos-tag テクノロジーの奥義2011

    • Author(s)
      木下英司
    • Organizer
      第63回日本生物工学会大会
    • Place of Presentation
      東京農工大学,東京都小金井市
    • Year and Date
      2011-09-28
    • Related Report
      2012 Final Research Report
  • [Presentation] リン酸化プロテオミクスに向けたPhos-tagテクノロジーの奥義2011

    • Author(s)
      木下英司
    • Organizer
      第63回日本生物工学会
    • Place of Presentation
      東京都小金井市(招待講演)
    • Year and Date
      2011-09-28
    • Related Report
      2011 Annual Research Report
  • [Presentation] Phos-tag を用いた分子イメージングとリン酸化シグナル研究2011

    • Author(s)
      木下英司
    • Organizer
      第84回日本生化学会大会
    • Place of Presentation
      国立京都国際会館,京都市
    • Year and Date
      2011-09-24
    • Related Report
      2012 Final Research Report
  • [Presentation] Phos-tagを用いた分子イメージングとリン酸化シグナル研究2011

    • Author(s)
      木下英司
    • Organizer
      第84回日本生化学会
    • Place of Presentation
      京都市
    • Year and Date
      2011-09-24
    • Related Report
      2011 Annual Research Report
  • [Presentation] 改良型Phos-tag Biotin を用いたペプチドマイクロアレイ解析による蛋白質リン酸化反応プロファイリング2011

    • Author(s)
      木下英司,木下恵美子,小池透
    • Organizer
      日本プロテオーム学会 2011 年大会
    • Place of Presentation
      朱鷺メッセ,新潟市
    • Year and Date
      2011-07-29
    • Related Report
      2012 Final Research Report
  • [Presentation] 改良型Phos-tag Biotinを用いたペプチドマイクロアレイ解析による蛋白質リン酸化反応プロファイリング2011

    • Author(s)
      木下英司
    • Organizer
      第9回日本プロテオーム学会
    • Place of Presentation
      新潟市
    • Year and Date
      2011-07-29
    • Related Report
      2011 Annual Research Report
  • [Presentation] Phos-tag を用いたリン酸化タンパク質解析の新展開2011

    • Author(s)
      木下英司
    • Organizer
      日本農芸化学会 2011 年大会ランチョンセミナー
    • Place of Presentation
      京都女子大学,京都市(招待講演)
    • Year and Date
      2011-03-26
    • Related Report
      2012 Final Research Report
  • [Presentation] Phos-tagを用いたリン酸化タンパク質解析の新展開2011

    • Author(s)
      木下英司
    • Organizer
      日本農芸化学会2011年大会
    • Place of Presentation
      京都女子大学(招待講演)
    • Year and Date
      2011-03-26
    • Related Report
      2010 Annual Research Report
  • [Presentation] Phos-tag がもたらすリン酸化シグナル研究のブレークスルー2011

    • Author(s)
      木下英司
    • Organizer
      酵母細胞プロジェクト研究センター春期シンポジウム
    • Place of Presentation
      広島大学大学院・先端物質科学研究科,東広島市
    • Year and Date
      2011-03-11
    • Related Report
      2012 Final Research Report
  • [Presentation] Phos-tagがもたらすリン酸化シグナル研究のブレークスルー2011

    • Author(s)
      木下英司
    • Organizer
      酵母細胞プロジェクト研究センター春期シンポジウム
    • Place of Presentation
      広島大学大学院先端物質科学研究科(招待講演)
    • Year and Date
      2011-03-11
    • Related Report
      2010 Annual Research Report
  • [Presentation] Phos-tag テクノロジーが拓く生命科学研究の新展開2011

    • Author(s)
      木下英司
    • Organizer
      第354回生命科学セミナー
    • Place of Presentation
      広島大学総合科学部,東広島市
    • Year and Date
      2011-02-23
    • Related Report
      2012 Final Research Report
  • [Presentation] Phos-tagテクノロジーが拓く生命科学研究の新展開2011

    • Author(s)
      木下英司
    • Organizer
      第354回生命科学セミナー
    • Place of Presentation
      広島大学総合科学部(招待講演)
    • Year and Date
      2011-02-23
    • Related Report
      2010 Annual Research Report
  • [Presentation] リン酸基認識素子としての二核亜鉛錯体とオミクス解析技術への応用2011

    • Author(s)
      木下英司
    • Organizer
      第4回日本化学会東海支部 若手研究者フォーラム
    • Place of Presentation
      名古屋市立大学薬学部,名古屋市
    • Year and Date
      2011-01-22
    • Related Report
      2012 Final Research Report
  • [Presentation] リン酸基認識素子としての二核亜鉛錯体とオミクス解析技術への応用2011

    • Author(s)
      木下英司
    • Organizer
      第4回日本化学会東海支部 若手研究者フォーラム
    • Place of Presentation
      名古屋市立大学薬学部(招待講演)
    • Year and Date
      2011-01-22
    • Related Report
      2010 Annual Research Report
  • [Presentation] Improved Phos-tag Biotin for advanced profiling of protein phosphorylation2010

    • Author(s)
      木下英司,木下恵美子,小池透
    • Organizer
      第33回日本分子生物学会年会・第83回日本生化学学会大会(BMB2010)
    • Place of Presentation
      神戸国際展示場,神戸市
    • Year and Date
      2010-12-09
    • Related Report
      2012 Final Research Report
  • [Presentation] Improved Phos-tag Biotin for advanced profiling of protein phosphorylation2010

    • Author(s)
      木下英司
    • Organizer
      第33回日本分子生物学会・第83回日本生化学学会年会
    • Place of Presentation
      神戸国際展示場
    • Year and Date
      2010-12-09
    • Related Report
      2010 Annual Research Report
  • [Presentation] Improved Phos-tag Biotin for highly sensitive detection of protein2010

    • Author(s)
      木下英司,木下恵美子,小池透
    • Organizer
      日本プロテオーム学会 2010 年大会
    • Place of Presentation
      東京ベイホテル東急,東京
    • Year and Date
      2010-07-27
    • Related Report
      2012 Final Research Report
  • [Presentation] Improved Phos-tag Biotin for highly sensitive detection of protein phosphorylation2010

    • Author(s)
      木下英司
    • Organizer
      日本プロテオーム学会第8回大会
    • Place of Presentation
      東京ベイホテル東急
    • Year and Date
      2010-07-27
    • Related Report
      2010 Annual Research Report
  • [Presentation] Phos-tagアフィニティ電気泳動の開発と改良~リン酸化タンパク質の分析

    • Author(s)
      木下英司
    • Organizer
      第62回日本電気泳動学会シンポジウム
    • Place of Presentation
      慶応義塾大学薬学部,東京
    • Related Report
      2012 Annual Research Report
  • [Presentation] 改良型Phos-tag Biotinの開発とリン酸化プロテオミクスへの応用

    • Author(s)
      木下英司
    • Organizer
      日本プロテオーム学会2012年大会
    • Place of Presentation
      日本科学未来館,東京
    • Related Report
      2012 Annual Research Report
  • [Presentation] リン酸化タンパク質解析のためのフォスタグテクノロジー

    • Author(s)
      木下英司
    • Organizer
      第63回日本電気泳動学会総会
    • Place of Presentation
      沖縄コンベンションセンター,宜野湾
    • Related Report
      2012 Annual Research Report
  • [Presentation] Phos-tag技術によって新たにできるようになったこと

    • Author(s)
      木下英司
    • Organizer
      新薬理学セミナー 2012
    • Place of Presentation
      熊本大学薬学部宮本記念館,熊本市
    • Related Report
      2012 Annual Research Report
    • Invited
  • [Presentation] SuperSep Phos-tag プレキャストゲル:迅速,高効率,かつ,高精度なリン酸化シグナル伝達分子の分離検出法

    • Author(s)
      木下英司
    • Organizer
      第35回日本分子生物学会年会
    • Place of Presentation
      マリンメッセ福岡,福岡
    • Related Report
      2012 Annual Research Report
  • [Presentation] Phos-tag 技術が拓くタンパク質リン酸化研究の新たな潮流

    • Author(s)
      木下英司
    • Organizer
      第85回日本生化学会大会シンポジウム
    • Place of Presentation
      福岡国際会議場,福岡
    • Related Report
      2012 Annual Research Report
  • [Presentation] Highly sensitive imaging of protein phosphorylation by Phos-tag technology

    • Author(s)
      Kinoshita, E.
    • Organizer
      1st International Symposium on Protein Modifications in Pathogenic Dysregulation of Signaling
    • Place of Presentation
      東大医科研講堂,東京
    • Related Report
      2012 Annual Research Report
  • [Book] Phos-tag affinity electrophoresis for protein kinase profiling2012

    • Author(s)
      Eiji Kinoshita, Kinoshita-Kikuta, E., and Koike, T
    • Publisher
      Springer
    • Related Report
      2012 Final Research Report
  • [Book] Phos-tag ゲルによるリン酸化タンパク質の分離・同定法.臨床プロテオミクス~バイオマーカー探索から個別化医療へ2012

    • Author(s)
      木下英司,木下恵美子,小池透
    • Publisher
      金原出版
    • Related Report
      2012 Final Research Report
  • [Book] Gel-based methods using DNA-binding zinc(II) complexes for the detection of DNA mutations. in DNA Replication and Mutation2012

    • Author(s)
      Eiji Kinoshita, Kinoshita-Kikuta, E and Koike, T
    • Publisher
      Nova Science Publishers
    • Related Report
      2012 Final Research Report
  • [Book] 実験医学2012

    • Author(s)
      木下英司,木下恵美子,小池透
    • Publisher
      羊土社
    • Related Report
      2012 Final Research Report
  • [Book] Phos-tag affinity electrophoresis for protein kinase profiling.2012

    • Author(s)
      Kinoshita, E.
    • Total Pages
      22
    • Publisher
      Springer
    • Related Report
      2012 Annual Research Report
  • [Book] 臨床プロテオミクス~バイオマーカー探索から個別化医療へ2012

    • Author(s)
      木下英司
    • Total Pages
      5
    • Publisher
      金原出版
    • Related Report
      2012 Annual Research Report
  • [Book] DNA Replication and Mutation2012

    • Author(s)
      Kinoshita, E.
    • Total Pages
      21
    • Publisher
      Nova Science Publishers
    • Related Report
      2012 Annual Research Report
  • [Book] 実験医学増刊号,羊土社2012

    • Author(s)
      木下英司
    • Total Pages
      7
    • Publisher
      リン酸基アフィニティ電気泳動法(Phos-tag SDS-PAGE)を用いたタンパク質リン酸化修飾の高感度検出
    • Related Report
      2011 Annual Research Report
  • [Book] DNA Replication and Mutation, Nova Science Publishers2011

    • Author(s)
      Kinoshita, E.
    • Total Pages
      22
    • Publisher
      Gel-Based Methods using DNA-Binding Zinc(II) Complexes for the Detection of DNA Mutations
    • Related Report
      2011 Annual Research Report
  • [Remarks]

    • URL

      http://home.hiroshima-u.ac.jp/tkoike/index.html

    • Related Report
      2012 Final Research Report
  • [Remarks]

    • URL

      http://shushoku-signal.com/soshiki/kobo/18kinoshita.html

    • Related Report
      2012 Final Research Report
  • [Remarks]

    • URL

      http://home.hiroshima-u.ac.jp/tkoike/index.html

    • Related Report
      2011 Annual Research Report
  • [Remarks]

    • URL

      http://www.phos-tag.com

    • Related Report
      2011 Annual Research Report
  • [Remarks]

    • URL

      http://home.hiroshima-u.ac.jp/tkoike/research.html

    • Related Report
      2010 Annual Research Report

URL: 

Published: 2010-08-23   Modified: 2019-07-29  

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