Analysis of phosphorylated biomolecules using a phosphate-affinity quartz-crystal microbalance method
Project/Area Number |
22590037
|
Research Category |
Grant-in-Aid for Scientific Research (C)
|
Allocation Type | Single-year Grants |
Section | 一般 |
Research Field |
Physical pharmacy
|
Research Institution | Hiroshima University |
Principal Investigator |
KINOSHITA Eiji 広島大学, 大学院・医歯薬保健学研究院, 准教授 (80304418)
|
Project Period (FY) |
2010 – 2012
|
Project Status |
Completed (Fiscal Year 2012)
|
Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2012: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2011: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2010: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
|
Keywords | リン酸化 / 水晶発振子マイクロバランス / QCM / バイオセンサー / フォスタグ / 分子認識 / 蛋白質 / 分析化学 |
Research Abstract |
In this study, the researcher has applied a novel phosphate capture molecule, biotinylated Phos-tag (Phos-tag Biotin BTL-111), which was originally synthesized, to a quartz-crystal microbalance procedure (QCM). By using the BTL-111,a phosphate-affinity QCM sensor chip has been newly developed. This novel biosensor chip permitted highly sensitive detections of phosphorylated biomolecules, such as phosphoproteins or DNA.
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Report
(4 results)
Research Products
(85 results)