Antioxidant activity evaluation of trace sample by the spot measurement of singlet oxygen luminescence lifetime
| Project/Area Number |
22590038
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Physical pharmacy
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| Research Institution | Ehime University |
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Principal Investigator |
OHARA Keishi 愛媛大学, 理工学研究科, 准教授 (10284390)
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| Project Period (FY) |
2010-04-01 – 2014-03-31
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2013: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2012: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2011: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2010: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
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| Keywords | 生体分子 / 化学物理 / 薬剤反応性 / 抗酸化 / 一重項酸素 / スポット計測 / 近赤外 |
|---|
| Research Abstract |
In order to evaluate antioxidant activities of trace amount of natural materials, a method of measuring lifetimes of singlet oxygen near-infrared luminescence using a fiber-spot probe (0.4 mm) was developed. Time-evolutions of singlet oxygen luminescence were successfully obtained from measurements of less than 0.1 mL of solutions and also hydrogel samples by the spot method. The quenching rate constants for trace amount of samples such as 0.05 mg of carotenoid isomers, and also that of the quencher in hydro-gel samples were determined. The relationship between singlet oxygen quenching activity and chemical structure for the lipophilic constituents of spices such as capsaicin were investigated in the cyclodextrin aqueous solution. However, in the spot measurements, the photo-damages often occurred in test materials and/or photosensitizers in the accumulation time (10 min). The shorter accumulation time (< 1 min) is hopeful for applying the spot-scan method to the antioxidant assay.
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Report
(5 results)
Research Products
(45 results)
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[Journal Article] Cyclo[8]isoindoles : Ring-Expanded and Annelated Porphyrinoids2011
Author(s)
T. Okujima, G. Jin, N. Matsumoto, J. Mack, S. Mori, K. Ohara, D. Kuzuhara, C. Ando, N. Ono, H. Yamada, H. Uno, N. Kobayashi
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Journal Title
Angewandte Chemie International Edition
Volume: vol.50
Issue: 25
Pages: 5699-5703
DOI
Related Report
Peer Reviewed
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[Journal Article] Synthesis, Crystal Structure, and Photodynamics ofπ-Expanded Porphyrin-Fullerene Dyads Synthesized by Diels-Alder Reaction2010
Author(s)
H. Yamada, K. Ohkubo, D. Kuzuhara, T. Takahashi, A.S.D. Sandanayaka, T. Okujima, K. Ohara, O. Ito, H. Uno, N. Ono, S. Fukuzumi
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Journal Title
The Journal of Physical Chemistry B
Volume: vol.114 (45)
Pages: 14717-14728
Related Report
Peer Reviewed
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[Presentation] Ni-dmit錯体の特異的に大きな光伝導性2014
Author(s)
内藤俊雄,烏谷知明,長山直樹,小原敬士,小西健介,森重樹,高野崇廣,高橋幸裕,稲辺保,木瀬翔太,西原禎文,井上克也
Organizer
日本化学会第94春季年会
Place of Presentation
名古屋
Related Report
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[Presentation] Synthesis of π-Expanded Cyclo[8]pyrroles2012
Author(s)
Tetsuo Okujima, Chie Ando, Naoki Matsumoto, Shigeki Mori, Guangnan Jin, Hiroko Yamada, Takahiro Nakae, Keishi Ohara, Hidemitsu Uno
Organizer
Seventh International Conference on Porphyrins and Phthalocyanines (ICPP-7)
Place of Presentation
Jeju, Korea
Related Report
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