Development of the analytical method for estrogen-o-quinones and its application to the study on the initiation mechanism of human breast cancer
| Project/Area Number |
22590041
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Physical pharmacy
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| Research Institution | Tohoku Pharmaceutical University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
OHNO Ken-ichi 東北薬科大学, 薬学部, 講師 (20347272)
KATO Hajime 東北薬科大学, 薬学部, 助教 (80584458)
KOMATSU Sachiko 東北薬科大学, 薬学部, 助手 (00438566)
沼澤 光輝 東北薬科大学, 薬学部, 教授 (90006338)
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| Project Period (FY) |
2010 – 2012
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| Project Status |
Completed (Fiscal Year 2012)
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| Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2012: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2011: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2010: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Keywords | 乳がん / エストロゲン / オルトキノン / フェナジン誘導体 / 質量分析 / 分析化学 / エストロゲンオルトキノン / 定量分析 / LC-MS / LC-ESI-MS/MS / ヘムタンパク |
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| Research Abstract |
Selective and sensitive analytical method for estrogen-o-quinones derived from catechol estrogen with enzymatic or non-enzymatic manner was developed based on the use of phenazine derivatization and LC-MS. The present method enabled to determine the catalytic activity of protein and some metal ions on the process of oxidation of catechol estrogens to the corresponding o-quinones. Furthermore, analytical method for the glutathione and L-cysteine conjugates of these o-quinones was established as one of the model for determining protein adducts of o-quinones.
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Report
(4 results)
Research Products
(25 results)
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[Journal Article] 18-Oxocortisol measurement in adrenal vein sampling as a biomarker for subclassifying primary aldosteroism2011
Author(s)
Y. Nakamura, F. Satoh, R. Morimoto, M. Kudo, K. Takase, C E. Gometz-Sanchez, S. Honma, M. Okuyama, K. Yamashita, W E. Rainey, H. Sasano, S.Ito
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Journal Title
J Clin Endocrinol Metab.
Volume: 96
DOI
Related Report
Peer Reviewed
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