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Development of the analytical method for estrogen-o-quinones and its application to the study on the initiation mechanism of human breast cancer

Research Project

Project/Area Number 22590041
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Physical pharmacy
Research InstitutionTohoku Pharmaceutical University

Principal Investigator

YAMASHITA Kouwa  東北薬科大学, 薬学部, 教授 (80382670)

Co-Investigator(Kenkyū-buntansha) OHNO Ken-ichi  東北薬科大学, 薬学部, 講師 (20347272)
KATO Hajime  東北薬科大学, 薬学部, 助教 (80584458)
KOMATSU Sachiko  東北薬科大学, 薬学部, 助手 (00438566)
沼澤 光輝  東北薬科大学, 薬学部, 教授 (90006338)
Project Period (FY) 2010 – 2012
Project Status Completed (Fiscal Year 2012)
Budget Amount *help
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2012: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2011: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2010: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Keywords乳がん / エストロゲン / オルトキノン / フェナジン誘導体 / 質量分析 / 分析化学 / エストロゲンオルトキノン / 定量分析 / LC-MS / LC-ESI-MS/MS / ヘムタンパク
Research Abstract

Selective and sensitive analytical method for estrogen-o-quinones derived from catechol estrogen with enzymatic or non-enzymatic manner was developed based on the use of phenazine derivatization and LC-MS. The present method enabled to determine the catalytic activity of protein and some metal ions on the process of oxidation of catechol estrogens to the corresponding o-quinones. Furthermore, analytical method for the glutathione and L-cysteine conjugates of these o-quinones was established as one of the model for determining protein adducts of o-quinones.

Report

(4 results)
  • 2012 Annual Research Report   Final Research Report ( PDF )
  • 2011 Annual Research Report
  • 2010 Annual Research Report

Research Products

(25 results)

All 2013 2012 2011 2010 Other

All Journal Article (5 results) (of which Peer Reviewed: 4 results) Presentation (14 results) Book (2 results) Remarks (4 results)

  • [Journal Article] Structures and biomimetic synthesis of a Novel α-pyrone polyketide of an endophytic Penicillium sp2013

    • Author(s)
      T, Asai, D. Luo, K. Yamashita, Y. Oshima
    • Journal Title

      Org. Letti

      Volume: 15 Pages: 1020-1023

    • DOI

      10.1021/ol303506t

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Structures and Biomimetic Synthesis of Novel a-Pyrone Polyketide of an Endophytic Penicillium sp. Catharanthus roseus2013

    • Author(s)
      Teigo Asai, Dan Luo, kouwa Yamashita, Yoshiteru Oshima
    • Journal Title

      Organic Letters

      Volume: 15 Pages: 1021-1023

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] 18-Oxocortisol measurement in adrenal vein sampling as a biomarker for subclassifying primary aldosteroism2011

    • Author(s)
      Y. Nakamura, F. Satoh, R. Morimoto, M. Kudo, K. Takase, C E. Gometz-Sanchez, S. Honma, M. Okuyama, K. Yamashita, W E. Rainey, H. Sasano, S.Ito
    • Journal Title

      J Clin Endocrinol Metab.

      Volume: 96

    • DOI

      10.1210/jc.2010-2785

    • Related Report
      2012 Final Research Report 2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Assay of labile estrogen o-quinones, a potent carcinogenic molecular species, by high performance liquid chromatographyelectrospray ionization mass spectrometry with phenazine derivatization2010

    • Author(s)
      K. Yamashita, A. Masuda, Y. Hoshino, S.Komatsu, M. Numazawa
    • Journal Title

      J. Steroid Biochem. Mol. Biol

      Volume: 119 Pages: 141-148

    • Related Report
      2012 Final Research Report
  • [Journal Article] Assay of labile estrogen-o-quinones, potent carcinogenic molecular species, by HPLC-electrosprayionization tandem mass spectrometry2010

    • Author(s)
      Kouwa Yamashita
    • Journal Title

      J.Steroid Biochem.Mol.Biol.

      Volume: 119 Pages: 141-148

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Presentation] LC-ESI-MS/MS法による水酸化ステロイドのメタボローム解析(V)安定同位体標識カテコールエストロゲン-GSH 付加体の合成2013

    • Author(s)
      山下幸和、近藤恵美、白取未希、小松祥子、加藤創、大野賢一、沼澤光輝
    • Organizer
      日本薬学会第133 年会
    • Place of Presentation
      横浜
    • Year and Date
      2013-03-28
    • Related Report
      2012 Final Research Report
  • [Presentation] LC-ESI-MS/MS法による水酸化ステロイドのメタボローム解析(V)安定同位体標識カテコールエストロゲン-GSH付加体の合成-2013

    • Author(s)
      山下幸和、近藤恵美、白取未希、小松祥子、加藤 創、大野賢一、沼澤光輝
    • Organizer
      日本薬学会第133年会
    • Place of Presentation
      横浜
    • Related Report
      2012 Annual Research Report
  • [Presentation] LC-ESI-MS/MS法による水酸化ステロイドのメタボローム解析(VI)-ステロイド硫酸抱合体の定量法の最適化-2013

    • Author(s)
      小松祥子、齋藤彩夏、榊 克教、山下幸和、沼澤光輝
    • Organizer
      日本薬学会第133年会
    • Place of Presentation
      横浜
    • Related Report
      2012 Annual Research Report
  • [Presentation] Pyridine-carboxylates as protonaffinitive derivatives in HPLC-ESI-MS/MS analysis of hydroxysteroids2012

    • Author(s)
      K. Yamashita, S. Komatsu, H. Kato, K.-I.Ohno
    • Organizer
      29^<th>LC/MS Montreux Symposium
    • Place of Presentation
      Montreux
    • Year and Date
      2012-11-09
    • Related Report
      2012 Final Research Report
  • [Presentation] LC-ESI-MS/MS によるアロマターゼ活性測定法の開発および従来法との比較2012

    • Author(s)
      小松祥子、鈴木直樹、高橋透、山下幸和、沼澤光輝
    • Organizer
      第51回日本薬学会東北支部大会
    • Place of Presentation
      青森
    • Year and Date
      2012-10-07
    • Related Report
      2012 Final Research Report
  • [Presentation] Use of proton-affinitive derivatization in HPLC-ESI-MS analysis of biologically important carboxylic acids2012

    • Author(s)
      H. Kato, S. Mitsuzuka, K. Yamashita, M. Numazawa
    • Organizer
      19^<th>International Mass Spectrometry Conference
    • Place of Presentation
      Kyoto
    • Year and Date
      2012-09-20
    • Related Report
      2012 Final Research Report
  • [Presentation] LC-ESI-MS/MSによる水酸化ステロイドのメタボロム解析(IV)27 ステロイドの一斉分析2012

    • Author(s)
      山下幸和、小松祥子、加藤創、大野賢一、沼澤光輝、本間誠次郎
    • Organizer
      日本薬学会第132年会
    • Place of Presentation
      札幌
    • Year and Date
      2012-03-30
    • Related Report
      2012 Final Research Report
  • [Presentation] LC-ESI-MS/MS法による水酸化ステロイドのメタボローム解析(IV)27ステロイドの一斉分析2012

    • Author(s)
      山下幸和、小松祥子、加藤創、大野賢一、沼澤光輝、本間誠次郎
    • Organizer
      日本薬学会第年会
    • Place of Presentation
      札幌(北海道大学)
    • Year and Date
      2012-03-30
    • Related Report
      2011 Annual Research Report
  • [Presentation] Use of proton-affinitive derivatization in HPLC-positive-ESI-MS analysis of biologically important carboxylic acids2012

    • Author(s)
      Hajime Kato, Shin Mitsuzuka, Kouwa Yamashita, Mitsuteru Numazawa
    • Organizer
      19th International Mass Spectrometry Conference
    • Place of Presentation
      京都
    • Related Report
      2012 Annual Research Report
  • [Presentation] LC-ESI-MS/MSによるアロマターゼ活性測定法の開発および従来法との比較2012

    • Author(s)
      小松祥子、鈴木直樹、高橋 透、山下幸和、沼澤光輝
    • Organizer
      第51回日本薬学会東北支部大会
    • Place of Presentation
      青森
    • Related Report
      2012 Annual Research Report
  • [Presentation] Pyridine-carboxylates as proton-affinitive derivatives in HPLC-ESI-MS/MS analysis of hydroxysteroids2012

    • Author(s)
      Kouwa Yamashita, Sachiko Komatsu, Hajime Katoh, Ken-ichi Ohno
    • Organizer
      29th LC/MS Montreux Symposium
    • Place of Presentation
      Switzerland
    • Related Report
      2012 Annual Research Report
  • [Presentation] エストロゲンオルトキノンによるタンパク修飾(I)-L-Cys, N-Ac-L-Cys 及び GSH-共有結合体の調製とカテコール/キノン平衡2011

    • Author(s)
      白取未希、加藤創、山下幸和、沼澤光輝
    • Organizer
      第50回日本薬学会東北支部大会
    • Place of Presentation
      仙台
    • Year and Date
      2011-10-30
    • Related Report
      2012 Final Research Report
  • [Presentation] An approach to the microdetermination of labile estrogen o-quinones by liquid chromatographyelectrospray ionization tandem mass spectrometry2010

    • Author(s)
      K. Yamashita, S. Komatsu, A. Masuda, M. Numazawa
    • Organizer
      58^<th>ASMS Confererence
    • Place of Presentation
      Salt Lake City, USA
    • Year and Date
      2010-05-25
    • Related Report
      2012 Final Research Report
  • [Presentation] An approach to the microdetermination of labile estrogen-o-quinones by liquid chromatography-electrospray ionization tandem mass spectrometry2010

    • Author(s)
      Kouwa Yamashita
    • Organizer
      58^<th> ASMS Conference on Mass Spectrometry and Allied Topics
    • Place of Presentation
      Salt Lake City, Utah USA
    • Year and Date
      2010-05-24
    • Related Report
      2010 Annual Research Report
  • [Book] Testosterone: Biochemistry, Therapeutic Uses and Physiological Effects2012

    • Author(s)
      K. Yamashita (ed. by Konstantin Chichinadze)
    • Total Pages
      193
    • Publisher
      Nova Scientific Publishers Inc
    • Related Report
      2012 Final Research Report
  • [Book] Testosterone: Biochemistry, Therapeutic Uses and Physiological Effects2012

    • Author(s)
      Kouwa Yamashita (ed. by Konstantin Chichinadze)
    • Total Pages
      193
    • Publisher
      Nova Scientific Publishers Inc
    • Related Report
      2012 Annual Research Report
  • [Remarks]

    • URL

      http://www.tohoku-pharm.ac.jp/laboratory/rinsyob/index.html

    • Related Report
      2012 Final Research Report
  • [Remarks] 東北薬科大学臨床分析化学教室ホームページ(教育研究組織)

    • URL

      http://www.tohoku-pharm.ac.jp/laboratory/rinsyob/index.html

    • Related Report
      2012 Annual Research Report
  • [Remarks]

    • URL

      http://www.tohoku-pharm.ac.jp/laboratory/rinsyob/ronbun.html

    • Related Report
      2011 Annual Research Report
  • [Remarks]

    • URL

      http://www.tohoku-pharm.ac.jp/laboratory/rinsyob/index.html

    • Related Report
      2010 Annual Research Report

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Published: 2010-08-22   Modified: 2019-07-29  

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