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photochemistry of sensory rhodopsin

Research Project

Project/Area Number 22590049
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Physical pharmacy
Research InstitutionHokkaido University (2012)
Matsuyama University (2010-2011)

Principal Investigator

KAMO Naoki  北海道大学, 名誉教授 (10001976)

Co-Investigator(Kenkyū-buntansha) NARA Toshifumi  松山大学, 薬学部, 准教授 (30241350)
Project Period (FY) 2010 – 2012
Project Status Completed (Fiscal Year 2012)
Budget Amount *help
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2012: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2011: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2010: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
Keywordsレチナールタンパク / 微生物ロドプシン / ホボロドプシン / ホトサイクルヒロロキシルアミン / レチナール異性化 / 高度好塩菌 / センソリーロドプシン / ブリーチ / カサノリ / ハロロドプシン / ホトサイクル / 走光性 / ロドプシン / レチナールタンパク質 / ヒドロキシルアミン / Natronomonas pharaonis / Halobacterium salinarum / バクテリオロドプシン / フォトサイクル / ラマンスペクトル / 光化学中間体 / N中間体 / Haloarcular marismortui
Research Abstract

In the membrane of Halobaterium salinarum, there exists sensory rhodopsin (SR) which acts as a photoreceptor of phototaxis of this bacterium. Photo-induced proton transfer of SR was made clear. So-called M-intermediate is considered to be functionally important, but we showed that this intermediate is fragile. We devised the apparatus which can monitor time-resolved photo-induced proton transfer. We first showed a so-called N-intermediate in SR. The photochemistry of SRIII was first clarified.

Report

(4 results)
  • 2012 Annual Research Report   Final Research Report ( PDF )
  • 2011 Annual Research Report
  • 2010 Annual Research Report
  • Research Products

    (30 results)

All 2013 2012 2011 2010 Other

All Journal Article (21 results) (of which Peer Reviewed: 21 results) Presentation (8 results) Remarks (1 results)

  • [Journal Article] Exporing the active site site structure of a photoreceptor protein by raman optical activity2013

    • Author(s)
      Unno Masashi, kikukawa takashi, Kumauchi Masato, Kamo Naoki
    • Journal Title

      Journal of Physical Chemistry.

      Volume: 117巻 Issue: 5 Pages: 1321-325

    • DOI

      10.1021/jp4001187

    • Related Report
      2012 Annual Research Report 2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Thermodynamic parameters of anionbinding to halorhodopsin from Natronomonas pharaonis by isothermal titration calorimetry2013

    • Author(s)
      Hayasi Saori, Tamogami Jun, Kikukawa Takashi, Okamoto Haruka, Shimono Kazumi, Miyauchi Seiji, Demura Makoto, Nara To shifumiKamo Naoki
    • Journal Title

      Biophysical Chemistry

      Volume: 172巻 Pages: 61-6710

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Salt bridge in the conserved His-Asp cluster in Gloeobacter rhodopsin contributes to trimer formation2013

    • Author(s)
      Tsukamoto, Takashi
    • Journal Title

      FEBS Letters

      Volume: 587 Issue: 3 Pages: 322-327

    • DOI

      10.1002/hep.26100

    • NAID

      120005228320

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Thermodynamic parameters of anion binding to halorhodopsin from Natronomonas pharaonis by isothermal titration calorimetry2013

    • Author(s)
      Hayashi, Saori
    • Journal Title

      Biophysical Chemistry

      Volume: 172 Pages: 61-67

    • DOI

      10.1016/j.bpc.2013.01.001

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Photoinduced proton release in proteorhodopsin at low pH: The possibility of a decrease in the pKa of Asp2272012

    • Author(s)
      Tamogami Jun, Kikukawa Takashi, NaraToshifumi , Shimono Kazumi, Demura Makoto, Kamo Naoki
    • Journal Title

      Biochemistry

      Volume: 51巻 Issue: 46 Pages: 9290-9301

    • DOI

      10.1021/bi300940p

    • Related Report
      2012 Annual Research Report 2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Photo-induced bleaching of sensory rhodopsin II (phoborhodopsin) from Halobaterium salinarum by hydroxylamine: Identification of the responsible intermediates.2012

    • Author(s)
      Tamogami Jun, Kikukawa Takashi, IkedaYoichi, Demura Makoto, NaraToshifumi , KamoNaoki
    • Journal Title

      Journal of Photochemistry and Photobiology B-Biology

      Volume: 106巻 Pages: 87-9410

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Homotrimer formation and dissociation of pharaonis halorhodopsin in detergent system2012

    • Author(s)
      Tsukamoto, Takashi
    • Journal Title

      Biophysical Journal

      Volume: 102 Pages: 2906-2915

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] "The influence of halide binding on the hydrogen bonding network in the active site of Salinibacter sensory rhodopsin I"2012

    • Author(s)
      L. Reissig, T. Iwata, T. Kikukawa, M. Demura, N. Kamo, H. Kandori and Y. Sudo
    • Journal Title

      Biochemistry

      Volume: 51 Issue: 44 Pages: 8802-8813

    • DOI

      10.1021/bi3009592

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Photo-induced bleaching of sensory rhodopsin II (phoborhodopsin) from Halobacteium salinarum by hydroxylamine2012

    • Author(s)
      Tamogami Jun
    • Journal Title

      Journal of Photochemistry and Photobiology B

      Volume: 106 Pages: 87-94

    • DOI

      10.1016/j.jphotobiol.2011.10.009

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Photochemistry of a putative new class of sensory rhodopsin (SRIII) coded by xop2 of Haloarcular marismortui2012

    • Author(s)
      Nakao Y, Kikukawa T, Shimono K, Tamogami J, Kimitsuki N, Nara T, Unno M, Ihara K, Kamo N.
    • Journal Title

      J Photochem Photobiol B

      Volume: 102 Issue: 1 Pages: 45-54

    • DOI

      10.1016/j.jphotobiol.2010.09.004

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Ramamoorthy Ayyalusamy, Naito Akira. An active photoreceptor intermediate revealed by in situ photoirradiated solid-state NMR spectroscopy2011

    • Author(s)
      Tomonaga Yuya, Hidaka Tetsuro, Kawamura Izuru, Nishio Takudo, Osawa Kazuhiro, Okitsu Takashi, Wada Akimori, Sudo Yuki, KamoNaoki
    • Journal Title

      Biophysical Journal

      Volume: 101巻

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] An Amino Acid Residue (S201) in the Retinal Binding Pocket Regulates the Photoreaction Pathway of Phoborhodopsin2011

    • Author(s)
      G.Dai, Y.Zhang, J.Tamogami, M.Demura, N.Kamo, H.Kandori, T.Iwasa
    • Journal Title

      Biochemistry

      Volume: 50 Issue: 33 Pages: 7177-7183

    • DOI

      10.1021/bi200598r

    • Related Report
      2012 Final Research Report 2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Spectroscopic evidence for the formation of an N-intermediate during the photocycle of sensory rhodopsin (phoborhodopsin) from Natromonaspharaonis2011

    • Author(s)
      Tateishi Yusuke, Abe Takayuki, Tamogami Jun, Nakao Yutaka, Kikukawa Takashi, KamoNaoki , Unno Masashi
    • Journal Title

      Biochemistry

      Volume: 50巻 Issue: 12 Pages: 2135-2143

    • DOI

      10.1021/bi1019572

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Expression of salinarum halorhodopsin in Escherichia coli cells : Solubilization in the presence of retinal yields the natural state2011

    • Author(s)
      Yamashita Yasutaka
    • Journal Title

      Biochimica et Biophysica Acta-Biomembranes

      Volume: 1808 Issue: 12 Pages: 2905-2912

    • DOI

      10.1016/j.bbamem.2011.08.035

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] An active photoreceptor intermediaterevealed by in situ photoirradiated Solid-State NMR spectroscopy2011

    • Author(s)
      Tomonaga Yuya
    • Journal Title

      Biophysical Journal

      Volume: 101

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Photochemistry of acetabularia rhodopsin II from a marine plant, Aceta bularia acetabulum2011

    • Author(s)
      Kikukawa Takashi
    • Journal Title

      Biochemistry

      Volume: 50 Issue: 41 Pages: 8888-8898

    • DOI

      10.1021/bi2009932

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Crystal structure of the Eukaryotic light-driven proton-pumping rhodopsin, Acetabularia rhodopsin II from Marine Algae2011

    • Author(s)
      Wada Takashi
    • Journal Title

      Journal of Molecular Biology

      Volume: 411 Issue: 5 Pages: 986-998

    • DOI

      10.1016/j.jmb.2011.06.028

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Spectroscopic evidence for the formation of an N-intermediate during the photocycle of sensory rhodopsin II (phoborhodopsin) from Natronobacterium2011

    • Author(s)
      Tateishi Yusuke
    • Journal Title

      Biochemistry

      Volume: 50 Pages: 2135-2143

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Spectroscopic evidence for the formation of an N-intermediate during the photocycle of sensory rhodpospin from Natronomonas pharaonis2011

    • Author(s)
      Tateishi, Y.
    • Journal Title

      Biochemistry

      Volume: 50 Pages: 2135-2143

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Photochemistry of a putative new class of sensory rhodopsin(SRIII)coded by xoP2 of Haloarcular marismortui2011

    • Author(s)
      Nakao, Y.
    • Journal Title

      Journal of Photochemistry and Photobiology B-Biology

      Volume: 102 Pages: 45-54

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] The photochemmical reaction cycle and photoinduced proton transfer of sensory rhodopsin II(phoborhodopsin II)from Halobacterium halobium2010

    • Author(s)
      Tamogami, J.
    • Journal Title

      Biophysical Journal

      Volume: 98 Pages: 1353-1363

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Presentation] When halorhodopsin does release anion2012

    • Author(s)
      Kikukawa, T., Kokubo, A., Tsukamoto, T., Ihara, K., Kamo , N ., Demura, N
    • Organizer
      15th International Conference on Retinal Proteins
    • Place of Presentation
      Monte Verita, Ascona, (Switzerland)
    • Year and Date
      2012-10-15
    • Related Report
      2012 Final Research Report
  • [Presentation] The role of chloride in the photo-induced proton transfer in sensory rhodopsin II from Natronomonas pharaoni2012

    • Author(s)
      Tamogami, J., Iwano, K., Matsuyama, A., Kikukawa, T., Demura, M., Shimono, K., Nara, T. , Kamo , N
    • Organizer
      第50回日本生物物理学会年会
    • Place of Presentation
      名古屋大学(名古屋市)
    • Year and Date
      2012-09-23
    • Related Report
      2012 Final Research Report
  • [Presentation] When halorhodpisn does release anions2012

    • Author(s)
      Kikukawa, Takashi
    • Organizer
      Interational Congress of Retinal Proteins 2012
    • Place of Presentation
      Monte Verita (Swizerland)
    • Related Report
      2012 Annual Research Report
  • [Presentation] 海洋細菌由来のロドプシン様タンパク質プロテオロドプシンの低pH条件下における速いプロトン放出機構2011

    • Author(s)
      田母神淳、菊川峰志,下野和実,奈良敏文,宗行英朗,加茂直樹
    • Organizer
      日本薬学会第132年会
    • Place of Presentation
      北海道大学(札幌市)
    • Year and Date
      2011-03-30
    • Related Report
      2012 Final Research Report
  • [Presentation] Laser flash analysis of functional interaction between sensory rhodopsin NpSRII (ppR) and eubacterial chemoreceptor Tsr in E. coli2010

    • Author(s)
      奈良敏文、菊川峰志、田母神淳、 加茂直樹
    • Organizer
      第48回日本生物物理学会
    • Place of Presentation
      東北大学(仙台市)
    • Year and Date
      2010-09-20
    • Related Report
      2012 Final Research Report
  • [Presentation] 負の走光性レセプターNpSRIIと大腸菌走化性レセプターTsrの機能的相互作用2010

    • Author(s)
      奈良敏文
    • Organizer
      日本生物物理学会
    • Place of Presentation
      東北大学川内北キャンパス
    • Year and Date
      2010-09-20
    • Related Report
      2010 Annual Research Report
  • [Presentation] センサリーロドプシンIIのヒドロキシルアミンによる退色反応2010

    • Author(s)
      田母神淳、菊川峰志、池田陽一、出村誠、加茂直樹
    • Organizer
      第10回蛋白質科学会年会
    • Place of Presentation
      札幌コンベンションセンター(札幌市)
    • Year and Date
      2010-06-18
    • Related Report
      2012 Final Research Report
  • [Presentation] センサリーロドプシンIIのヒドロキシルアミンによる退色反応2010

    • Author(s)
      田母神淳、菊川峰志、池田陽一、出村誠、加茂直樹
    • Organizer
      題10回蛋白質科学会年会
    • Place of Presentation
      札幌コンベンションセンター(札幌市)
    • Year and Date
      2010-06-18
    • Related Report
      2012 Final Research Report
  • [Remarks] 現在名誉教授なので、ホームページはない。一昨年度まで在籍していた松山大学

    • URL

      http://ghp01.matsuyama-u.ac.jp/~yakugaku/laboratory/labo_biophysical-chemistry.html

    • Related Report
      2012 Final Research Report

URL: 

Published: 2010-08-23   Modified: 2019-07-29  

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