Spatiotemporal regulation of phagocytosis by phosphoinositides;fluorescent microscopic analysis with shRNA knock down cells
| Project/Area Number |
22590065
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Biological pharmacy
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| Research Institution | Hiroshima University |
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Principal Investigator |
HAZEKI Kaoru 広島大学, 大学院・医歯薬学保健学研究院, 准教授 (50146007)
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| Project Period (FY) |
2010 – 2012
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| Project Status |
Completed (Fiscal Year 2012)
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| Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2012: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2011: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2010: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Keywords | イノシトールリン脂質 / shRNA / マクロファージ / 貪食 / ファゴソーム / タイムラプスイメージング / RNA干渉 |
|---|
| Research Abstract |
A series of cells lacking phosphoinositide-metabolizing enzymes was prepared from Raw264.7 macrophages. The cells were transfected with fluorescent probes, which bind to specific phosphoinositides. The life spans of various phosphoinositides on phagosome during phagocytosis in these cells were monitored by time-pulse imaging. Thus the enzymes responsible for the life spans of the phosphoinsitides were determined. The results will contribute to clarify the mechanism involved in autophagy and intracellular trafficking.
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Report
(4 results)
Research Products
(28 results)
