Visualization ofβ3 adrenoceptor signaling for development of ligand screening.

• Project/Area Number: 22590069
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Biological pharmacy
• Research Institution: Iwate Medical University
• Principal Investigator: FUJIMOTO Yasuyuki 岩手医科大学, 薬学部, 准教授 (60317724)
• Project Period (FY): 2010 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2012: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2011: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2010: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
• Keywords: アドレナリン受容体 / 蛍光 / 脂肪滴 / 受容体

Research Abstract

β3 adrenergic receptor acts for transmission ofadrenergic signaling on the cell surface of adipocytes activating hydrolysis of neutral lipids stored in the cells. In this study, I tried visualization of signaling mediated by β3 adrenoceptor. For this purpose, β3 adrenergic receptor, βarrestin2 and perilipin were fused withfluorescent proteins and expressed in mammalian cells. Distributions of fluorescence of these proteins were observed with microscopes.

Research Products

• [Journal Article] Transporter Associated with Antigen Processing-Like (ABCB9) Stably Expressed in Chinese Hamster Ovary-K1 Cells Is Sorted to the Microdomains of Lysosomal Membranes (2011)
  • Author(s): Fujimoto Y., Kamakura A., Motohashi Y., Ohashi-Kobayashi A. and Maeda M
  • Journal Title: Biological and Pharmaceutical Bulletin Volume: 34 Issue: 1 Pages: 36-40
  • DOI: 10.1248/bpb.34.36
  • NAID: 130000402259
  • ISSN: 0918-6158, 1347-5215
  • Peer Reviewed
• [Journal Article] Convergent synthesis of fluorescence-labeled probes of Annonaceous acetogenins and visualization of their cell distribution (2010)
  • Author(s): Kojima N, Morioka T, Urabe D, Yano M, Suga Y, Maezaki N, Ohashi-Kobayashi A, Fujimoto Y, Maeda M, Yamori T, Yoshimitsu T, Tanaka T
  • Journal Title: Bioorg Med Chem Volume: 18(24) Issue: 24 Pages: 8630-8641
  • DOI: 10.1016/j.bmc.2010.10.004
  • Peer Reviewed
• [Journal Article] Convergent synthesis of fluorescence-labeled probes of Annonaceous acetogenins and visualization of their cell distribution. (2010)
  • Author(s): Kojima N., Morioka T., Urabe D., Yano M, Suga Y., Maezaki N., Ohashi-Kobayashi A., Fujimoto Y., Maeda M., Yamori T., Yoshimitsu T., Tanaka T.
  • Journal Title: Bioorg.Med.Chem. Volume: 18(24) Pages: 8630-8641
  • Peer Reviewed
• [Presentation] Escherichia coliATP合成酵素変異型cサブユニットの抑圧変異の解析 (2012)
  • Author(s): 高橋翔太、荒木信、藤本康之、前田正知
  • Organizer: 第51回日本薬学会東北支部大会
  • Place of Presentation: 青森市
  • Year and Date: 2012-10-07
• [Presentation] ABC 輸送体 TAPL (ABCB9) のlysosome 局在化におけるN 末端領域膜貫通ドメインの寄与 (2011)
  • Author(s): 藤本康之、前田正知
  • Organizer: 第84回日本生化学会大会
  • Place of Presentation: 京都市
  • Year and Date: 2011-09-22
• [Presentation] ABC輸送体TAPL (ABCB9)のlysosome局在化におけるN末端領域膜貫通ドメインの寄与 (2011)
  • Author(s): 藤本康之、前田正知
  • Organizer: 第84回日本生化学会大会
  • Place of Presentation: 京都市
  • Year and Date: 2011-09-22
• [Presentation] Lysosomal targeting of ABC transporter TAPL (Transporter Associated with Antigen Processing Like) 招待公演 (2011)
  • Author(s): Maeda M. and Fujimoto Y.
  • Organizer: BIT's 4th Annual Protein and Peptide Conference
  • Place of Presentation: Beijing China
  • Year and Date: 2011-03-24
• [Presentation] Lysosomal targeting of ABC transporter TAPL (Transporter Associated with Antigen Processing Like) (2011)
  • Author(s): Maeda M., Fujimoto Y.
  • Organizer: BIT's 4^<th> Annual Protein and Peptide Conference
  • Place of Presentation: Beijing China(招待公演)
  • Year and Date: 2011-03-24
• [Presentation] ABC 輸送体 TAPL(ABCB9)の lysosome 局在化における N末端領域の寄与 (2010)
  • Author(s): 藤本康之、前田正知
  • Organizer: 第33回日本分子生物学会年会・第83回日本生化学会大会 合同大会
  • Place of Presentation: 神戸市
  • Year and Date: 2010-12-08
• [Presentation] ABC輸送体TAPL(ABCB9)のlysosome局在化におけるN末端領域の寄与 (2010)
  • Author(s): 藤本康之、前田正知
  • Organizer: 第33回日本分子生物学会年会・第83回日本生化学会大会 合同大会
  • Place of Presentation: 神戸国際会議場
  • Year and Date: 2010-12-08
• [Presentation] Escherichia coli ATP合成酵素変異型cサブユニットの抑圧変異の解析
  • Author(s): 高橋翔太，荒木信，藤本康之，前田正知
  • Organizer: 第51回 日本薬学会東北支部大会
  • Place of Presentation: 青森大学薬学部