Visualization ofβ3 adrenoceptor signaling for development of ligand screening.
| Project/Area Number |
22590069
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Biological pharmacy
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| Research Institution | Iwate Medical University |
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Principal Investigator |
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| Project Period (FY) |
2010 – 2012
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| Project Status |
Completed (Fiscal Year 2012)
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| Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2012: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2011: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2010: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Keywords | アドレナリン受容体 / 蛍光 / 脂肪滴 / 受容体 |
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| Research Abstract |
β3 adrenergic receptor acts for transmission ofadrenergic signaling on the cell surface of adipocytes activating hydrolysis of neutral lipids stored in the cells. In this study, I tried visualization of signaling mediated by β3 adrenoceptor. For this purpose, β3 adrenergic receptor, βarrestin2 and perilipin were fused withfluorescent proteins and expressed in mammalian cells. Distributions of fluorescence of these proteins were observed with microscopes.
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Report
(4 results)
Research Products
(11 results)
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[Journal Article] Convergent synthesis of fluorescence-labeled probes of Annonaceous acetogenins and visualization of their cell distribution2010
Author(s)
Kojima N, Morioka T, Urabe D, Yano M, Suga Y, Maezaki N, Ohashi-Kobayashi A, Fujimoto Y, Maeda M, Yamori T, Yoshimitsu T, Tanaka T
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Journal Title
Bioorg Med Chem
Volume: 18(24)
Issue: 24
Pages: 8630-8641
DOI
Related Report
Peer Reviewed
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[Journal Article] Convergent synthesis of fluorescence-labeled probes of Annonaceous acetogenins and visualization of their cell distribution.2010
Author(s)
Kojima N., Morioka T., Urabe D., Yano M, Suga Y., Maezaki N., Ohashi-Kobayashi A., Fujimoto Y., Maeda M., Yamori T., Yoshimitsu T., Tanaka T.
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Journal Title
Bioorg.Med.Chem.
Volume: 18(24)
Pages: 8630-8641
Related Report
Peer Reviewed
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