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Searching for genetic factors involved in formation and maturation of novel lysosome-related granules in Caenorhabditis elegans intestine.

Research Project

Project/Area Number 22590070
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Biological pharmacy
Research InstitutionIwate Medical University

Principal Investigator

OHASHI Ayako  岩手医科大学, 薬学部, 教授 (90272484)

Co-Investigator(Renkei-kenkyūsha) SHIRAISHI Hirohisa  岩手医科大学, 薬学部, 講師 (80393156)
TANJI Takahiro  岩手医科大学, 薬学部, 助教 (60453320)
NISHIKORI Kenji  岩手医科大学, 薬学部, 助教 (20563844)
Project Period (FY) 2010 – 2012
Project Status Completed (Fiscal Year 2012)
Budget Amount *help
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2011: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2010: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Keywordsゲノム / 薬学 / バイオテクノロジー / 遺伝学 / 細胞組織 / 線虫 / 腸 / 細胞機能 / RNAi / 遺伝子スクリーニング
Research Abstract

A systemic RNAi screening methods was developed for searching genetic factors involved in the formation and maturation of novel lysosome-related intestinal granules in Caenorhabditis elegans. The screening system identified various types of genes which affect the intestinal granular formationand maturation. Genetic and biochemical interaction between HAF-4 and HAF-9, the first two identified ABC transporter genes to be required for the intestinal granular formation, was also analyzed.

Report

(4 results)
  • 2012 Annual Research Report   Final Research Report ( PDF )
  • 2011 Annual Research Report
  • 2010 Annual Research Report

Research Products

(39 results)

All 2013 2012 2011 2010 Other

All Journal Article Presentation Book Remarks

  • [Journal Article] Co-operative function and mutual stabilization of the half-type ATP- binding cassette transporters HAF-4 and HAF-92013

    • Author(s)
      Tanji T, Nishikori K, Shiraishi H,Maeda M, Ohashi-Kobayashi A.
    • Journal Title

      Biochem J.

      Volume: (in press) Pages: 467-475

    • DOI
      10.1042/bj20130115
    • Related Report
      2012 Annual Research Report 2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Characterization of unusually reduced size of GATA-6 without a PEST sequence.2012

    • Author(s)
      Obayashi K, Takeda K, Ohashi K, Kobayashi-Ohashi A, Maeda M.
    • Journal Title

      Advances in Bioscience and Biotechnology.

      Volume: 3 Pages: 314-320

    • DOI
      10.4236/abb.2012.34045
    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Role of the PEST sequence in the long-type GATA-6 DNA-binding protein expressed in human cancer cell lines.2012

    • Author(s)
      Obayashi K.
    • Journal Title

      Adv. Biosci. Biotechnol.

      Volume: 3 Pages: 314-320

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Transporter associated with antigen processing-like (ABCB9) stably expressed in CHO-K1 cells is sorted to the microdomains of lysosomal membranes.2011

    • Author(s)
      Fujimoto Y, Kamakura A, Motohashi Y, Ohashi-Kobayashi A, Maeda M.
    • Journal Title

      Biol. Pharm. Bull

      Volume: 34 Pages: 36-40

    • URL
      https://wwwjstage.jst.go.jp/article/bpb/34/1/34_1_36/_article
    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Tissue and developmental expression of SRAM, an unconventional Rel-family protein2011

    • Author(s)
      Shiraishi H, Tanji T, Natori S, Ohashi-Kobayashi A.
    • Journal Title

      Arch. Insect. Biochem. Physiol.

      Volume: 76 Pages: 22-29

    • DOI
      10.1002/arch.20400
    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Transporter associated with antigen processing-like (ABCB9) stably expressed in CHO-K1 cells is sorted to the microdomains of lysosomal membranes.2011

    • Author(s)
      Fujimoto Y, et al.
    • Journal Title

      Biol.Pharm.Bull.

      Volume: 34 Pages: 36-40

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Tissue and developmental expression of SRAM, an unconventional Rel-family protein.2011

    • Author(s)
      Shiraishi H, et al
    • Journal Title

      Arch Insect Biochem Physiol.

      Volume: 76 Pages: 22-29

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Convergent synthesis of fluorescence-labeled probes of Annonaceousacetogenins and visualization of their cell distribution2010

    • Author(s)
      Kojima N, Morioka T, Urabe D, Yano M, Suga Y, Maezaki N, Ohashi-Kobayashi A, Fujimoto Y, Maeda M, Yamori T, Yoshimitsu T, Tanaka T
    • Journal Title

      Bioorg. Med. Chem.

      Volume: 18(24) Pages: 8630-8641

    • DOI
      10.1016/j.bmc.2010.10.004
    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Convergent synthesis of fluorescence-labeled probes of Annonaceous acetogenins and visualization of their cell distribution.2010

    • Author(s)
      Kojima N, et al.
    • Journal Title

      Bioorg.Med Chem.

      Volume: 18 Pages: 8630-8641

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Presentation] 線虫の腸細胞オルガネラに局在するABC輸送体HAF-4とHAF-9の多量体形成と相互安定化2013

    • Author(s)
      丹治貴博 他
    • Organizer
      日本薬学会第133年会
    • Place of Presentation
      横浜
    • Related Report
      2012 Annual Research Report 2012 Final Research Report
  • [Presentation] 線虫腸細胞に存在するABC輸送体HAF-4・HAF-9陽性オルガネラの栄養環境に依存した変動2013

    • Author(s)
      黒田英介 他
    • Organizer
      日本薬学会第133年会
    • Place of Presentation
      横浜
    • Related Report
      2012 Annual Research Report 2012 Final Research Report
  • [Presentation] Age- and nutrition-dependent changes of intestinal granules in C.elegans加齢および栄養状態に依存した線虫腸内顆粒の変動2012

    • Author(s)
      丹治貴博、錦織健児、上田佑毅、黒田英介、青山玲子、遠山稿二郎、白石博久、大橋綾子
    • Organizer
      第6回先端医療薬学研究センター講演会
    • Place of Presentation
      岩手医科大学(矢巾)
    • Year and Date
      2012-03-16
    • Related Report
      2011 Annual Research Report
  • [Presentation] Proteomic and genetic approaches to the mechanisms underlying the formation of intestinal granules in C.elegansプロテオミクス及び遺伝学的アプローチによる線虫腸内顆粒の形成機構の解析2012

    • Author(s)
      錦織健児、丹治貴博、淺沼優美、及川嵩人、菊池里美、田邉麻莉、加茂政晴、石崎明、白石博久、大橋綾子
    • Organizer
      第6回先端医療薬学研究センター講演会
    • Place of Presentation
      岩手医科大学(矢巾)
    • Year and Date
      2012-03-16
    • Related Report
      2011 Annual Research Report
  • [Presentation] Identification of proteins affected by the deletion for haf-4and haf-9, the intestinal ABC transporter genes in Caenorhabditis elegans.2012

    • Author(s)
      Kenji Nishikoriet al
    • Organizer
      New Frontiers of Metabolism Research in Biomedical Sciences
    • Place of Presentation
      東京
    • Related Report
      2012 Final Research Report
  • [Presentation] Dynamic changes of major large intestinal granules in response to aging and starvation in Caenorhabditis elegans2012

    • Author(s)
      Hirohisa Shiraishi et al.
    • Organizer
      New Frontiers of Metabolism Research in BiomedicalSciences
    • Place of Presentation
      東京
    • Related Report
      2012 Final Research Report
  • [Presentation] Age-dependent alteration of major large intestinal granules in Caenorhabditis elegans.2012

    • Author(s)
      Ayako Ohashi-Kobayashi et al.
    • Organizer
      Aging, Metabolism, Stress, Pathogenesis, and Small RNAs in C. elegans Topic Meeting
    • Place of Presentation
      マディソン,米国
    • Related Report
      2012 Final Research Report
  • [Presentation] Physical interaction of half ABC transporters HAF-4 and HAF-9, which are required for the biogenesis of the intestinal lysosome-related organelles in Caenorhabditis elegans.2012

    • Author(s)
      Takahiro Tanji et al.
    • Organizer
      Aging, Metabolism, Stress, Pathogenesis, and Small RNAs in C. elegans Topic Meeting
    • Place of Presentation
      マディソン,米国
    • Related Report
      2012 Final Research Report
  • [Presentation] Age-dependent alteration of major large intestinal granules in Caenorhabditis elegans.2012

    • Author(s)
      Ohashi-Kobayashi A.
    • Organizer
      Aging, Metabolism, Stress, Pathogenesis, and Small RNAs in C. elegans Topic Meeting
    • Place of Presentation
      マディソン, 米国
    • Related Report
      2012 Annual Research Report
  • [Presentation] Physical interaction of half ABC transporters HAF-4 and HAF-9, which are required for the biogenesis of the intestinal lysosome-related organe2012

    • Author(s)
      Tanji T.
    • Organizer
      Aging, Metabolism, Stress, Pathogenesis, and Small RNAs in C. elegans Topic Meeting
    • Place of Presentation
      マディソン, 米国
    • Related Report
      2012 Annual Research Report
  • [Presentation] Dynamic changes of major large intestinal granules in response to aging and starvation in Caenorhabditis elegans.2012

    • Author(s)
      Shiraishi H.
    • Organizer
      New Frontiers of Metabolism Research in Biomedical Sciences
    • Place of Presentation
      東京
    • Related Report
      2012 Annual Research Report
  • [Presentation] Identification of proteins affected by the deletion for haf-4 and haf-9, the intestinal ABC transporter genes in Caenorhabditis elegans.2012

    • Author(s)
      Nishikori K.
    • Organizer
      New Frontiers of Metabolism Research in Biomedical Sciences
    • Place of Presentation
      東京
    • Related Report
      2012 Annual Research Report
  • [Presentation] 成虫期の線虫では加齢に伴った腸内顆粒のリニューアルが起こる2011

    • Author(s)
      錦織健児、丹治貴博、白石博久、大橋綾子
    • Organizer
      第34回日本分子生物学会年会
    • Place of Presentation
      パシフィコ横浜(横浜)
    • Year and Date
      2011-12-15
    • Related Report
      2011 Annual Research Report
  • [Presentation] Mutual stabilization of half-type ABC transporters HAF-4 and HAF-9 in Caenorhabditis elegans2011

    • Author(s)
      丹治貴博、白石博久、錦織健児、前田正知、大橋綾子
    • Organizer
      第84回日本生化学会大会
    • Place of Presentation
      京都国際会議場(京都)
    • Year and Date
      2011-09-22
    • Related Report
      2011 Annual Research Report
  • [Presentation] プロテオミクスによる線虫(C.elegans)リソソーム関連ABC輸送体(HAF-4, HAF-9)の生理機能の解析2011

    • Author(s)
      錦織健児、丹治貴博、白石博久、大橋綾子
    • Organizer
      第6回トランスポーター研究会年会
    • Place of Presentation
      東北大学(仙台)
    • Year and Date
      2011-06-11
    • Related Report
      2011 Annual Research Report
  • [Presentation] 成虫期の線虫では加齢に伴った腸内顆粒のリニューアルが起こる2011

    • Author(s)
      錦織健児 他
    • Organizer
      第34回日本分子生物学会年会
    • Place of Presentation
      横浜
    • Related Report
      2012 Final Research Report
  • [Presentation] Mutual stabilization of half-type ABC transporters HAF-4 and HAF-9 in Caenorhabditis elegans.2011

    • Author(s)
      Takahiro Tanji et al.
    • Organizer
      第84回日本生化学会大会
    • Place of Presentation
      京都
    • Related Report
      2012 Final Research Report
  • [Presentation] プロテオミクスによる線虫(C. elegans) リソソーム関連ABC輸送体(HAF-4, HAF-9)の生理機能の解析2011

    • Author(s)
      錦織健児 他
    • Organizer
      第6回トランスポーター研究会年会
    • Place of Presentation
      仙台
    • Related Report
      2012 Final Research Report
  • [Presentation] Changes of protein profiles in the deletion mutant for haf-4 and haf-9, the intestinal ABC trapsporter genes in C.elegans2010

    • Author(s)
      Nishikori K, et al.
    • Organizer
      BMB2010
    • Place of Presentation
      神戸
    • Year and Date
      2010-12-09
    • Related Report
      2010 Annual Research Report
  • [Presentation] Characterization of HAF-2 as a lysosomal ABC transporter in Caenorhabditis elegans.2010

    • Author(s)
      Shiraishi H, et al.
    • Organizer
      East Asia Worm Meeting 2010
    • Place of Presentation
      東京
    • Year and Date
      2010-07-12
    • Related Report
      2010 Annual Research Report
  • [Presentation] Genetic interaction of HAF-4 and HAF-9, half-type ABC transporters required for the biogenesis of intestinal lysosome-related organelle in Caenorhabditis elegans.2010

    • Author(s)
      Tanji T, et al.
    • Organizer
      East Asia Worm Meeting 2010
    • Place of Presentation
      東京
    • Year and Date
      2010-07-12
    • Related Report
      2010 Annual Research Report
  • [Presentation] ATP-binding cassette transporters HAF-4 and HAF-9, homologues of human lysosomal peptide transporter TAP-like are required for normal formation of a subset of intestinal granules in Caenorhabditis elegans.2010

    • Author(s)
      Nishikori K, et al.
    • Organizer
      East Asia Worm Meeting 2010
    • Place of Presentation
      東京
    • Year and Date
      2010-07-11
    • Related Report
      2010 Annual Research Report
  • [Presentation] Genetic and physical interactions between half-type ABC transporters HAF-4 and HAF-9, which are required for the biogenesis of intestinal lysosome-related organelle in Caenorhabditis elegans.2010

    • Author(s)
      Takahiro Tanji et al
    • Organizer
      BMB2010
    • Place of Presentation
      神戸
    • Related Report
      2012 Final Research Report 2010 Annual Research Report
  • [Presentation] Changes of protein profiles in the deletion mutant for haf-4 and haf-9, the intestinal ABC transporter genes in C. elegans.2010

    • Author(s)
      Kenji Nishikori et al
    • Organizer
      BMB2010
    • Place of Presentation
      神戸
    • Related Report
      2012 Final Research Report
  • [Presentation] ATP-binding cassette transporters HAF-4 and HAF-9, homologues of human lysosomal peptide transporter TAP-like are required for biogenesis of a subset of intestinal granules in Caenorhabditis elegans.2010

    • Author(s)
      Kenji Nishikori et al
    • Organizer
      4th East Asia C. elegans Meeting
    • Place of Presentation
      東京
    • Related Report
      2012 Final Research Report
  • [Presentation] Genetic interaction of HAF-4 and HAF-9, half-type ABC transporters required for the biogenesis of intestinal lysosome-related organelle in Caenorhabditis elegans.2010

    • Author(s)
      Takahiro Tanji et al
    • Organizer
      4th East Asia C. elegans Meeting
    • Place of Presentation
      東京
    • Related Report
      2012 Final Research Report
  • [Presentation] Characterization of HAF-2 as a lysosomal ABC transporter in Caenorhabditis elegans.2010

    • Author(s)
      Hirohisa Shiraishi et al
    • Organizer
      4th East Asia C. elegans Meeting
    • Place of Presentation
      東京
    • Related Report
      2012 Final Research Report
  • [Book] 生物学大辞典2010

    • Author(s)
      大橋綾子(分担執筆)
    • Publisher
      東京化学同人
    • Related Report
      2012 Final Research Report 2010 Annual Research Report
  • [Remarks]

    • URL
      http://gaia-sb.iwate-med.ac.jp/pharm/?page_id=42
    • Related Report
      2012 Final Research Report
  • [Remarks] 岩手医科大学薬学部生体防御学講座HP

    • URL
      http://gaia-sb.iwate-med.ac.jp/pharm/?page_id=42
    • Related Report
      2012 Annual Research Report

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Published: 2010-08-23   Modified: 2019-07-29  

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