Searching for genetic factors involved in formation and maturation of novel lysosome-related granules in Caenorhabditis elegans intestine.

• Project/Area Number: 22590070
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Biological pharmacy
• Research Institution: Iwate Medical University
• Principal Investigator: OHASHI Ayako 岩手医科大学, 薬学部, 教授 (90272484)
• Co-Investigator(Renkei-kenkyūsha): SHIRAISHI Hirohisa 岩手医科大学, 薬学部, 講師 (80393156) ; TANJI Takahiro 岩手医科大学, 薬学部, 助教 (60453320) ; NISHIKORI Kenji 岩手医科大学, 薬学部, 助教 (20563844)
• Project Period (FY): 2010 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000); Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2011: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2010: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
• Keywords: ゲノム / 薬学 / バイオテクノロジー / 遺伝学 / 細胞組織 / 線虫 / 腸 / 細胞機能 / RNAi / 遺伝子スクリーニング

Research Abstract

A systemic RNAi screening methods was developed for searching genetic factors involved in the formation and maturation of novel lysosome-related intestinal granules in Caenorhabditis elegans. The screening system identified various types of genes which affect the intestinal granular formationand maturation. Genetic and biochemical interaction between HAF-4 and HAF-9, the first two identified ABC transporter genes to be required for the intestinal granular formation, was also analyzed.

Research Products

• [Journal Article] Co-operative function and mutual stabilization of the half-type ATP- binding cassette transporters HAF-4 and HAF-9 (2013)
  • Author(s): Tanji T, Nishikori K, Shiraishi H,Maeda M, Ohashi-Kobayashi A.
  • Journal Title: Biochem J. Volume: (in press) Pages: 467-475
  • DOI: 10.1042/bj20130115
  • Peer Reviewed
• [Journal Article] Characterization of unusually reduced size of GATA-6 without a PEST sequence. (2012)
  • Author(s): Obayashi K, Takeda K, Ohashi K, Kobayashi-Ohashi A, Maeda M.
  • Journal Title: Advances in Bioscience and Biotechnology. Volume: 3 Pages: 314-320
  • DOI: 10.4236/abb.2012.34045
  • Peer Reviewed
• [Journal Article] Role of the PEST sequence in the long-type GATA-6 DNA-binding protein expressed in human cancer cell lines. (2012)
  • Author(s): Obayashi K.
  • Journal Title: Adv. Biosci. Biotechnol. Volume: 3 Pages: 314-320
  • Peer Reviewed
• [Journal Article] Transporter associated with antigen processing-like (ABCB9) stably expressed in CHO-K1 cells is sorted to the microdomains of lysosomal membranes. (2011)
  • Author(s): Fujimoto Y, Kamakura A, Motohashi Y, Ohashi-Kobayashi A, Maeda M.
  • Journal Title: Biol. Pharm. Bull Volume: 34 Pages: 36-40
  • URL: https://wwwjstage.jst.go.jp/article/bpb/34/1/34_1_36/_article
  • Peer Reviewed
• [Journal Article] Tissue and developmental expression of SRAM, an unconventional Rel-family protein (2011)
  • Author(s): Shiraishi H, Tanji T, Natori S, Ohashi-Kobayashi A.
  • Journal Title: Arch. Insect. Biochem. Physiol. Volume: 76 Pages: 22-29
  • DOI: 10.1002/arch.20400
  • Peer Reviewed
• [Journal Article] Transporter associated with antigen processing-like (ABCB9) stably expressed in CHO-K1 cells is sorted to the microdomains of lysosomal membranes. (2011)
  • Author(s): Fujimoto Y, et al.
  • Journal Title: Biol.Pharm.Bull. Volume: 34 Pages: 36-40
  • Peer Reviewed
• [Journal Article] Tissue and developmental expression of SRAM, an unconventional Rel-family protein. (2011)
  • Author(s): Shiraishi H, et al
  • Journal Title: Arch Insect Biochem Physiol. Volume: 76 Pages: 22-29
  • Peer Reviewed
• [Journal Article] Convergent synthesis of fluorescence-labeled probes of Annonaceousacetogenins and visualization of their cell distribution (2010)
  • Author(s): Kojima N, Morioka T, Urabe D, Yano M, Suga Y, Maezaki N, Ohashi-Kobayashi A, Fujimoto Y, Maeda M, Yamori T, Yoshimitsu T, Tanaka T
  • Journal Title: Bioorg. Med. Chem. Volume: 18(24) Pages: 8630-8641
  • DOI: 10.1016/j.bmc.2010.10.004
  • Peer Reviewed
• [Journal Article] Convergent synthesis of fluorescence-labeled probes of Annonaceous acetogenins and visualization of their cell distribution. (2010)
  • Author(s): Kojima N, et al.
  • Journal Title: Bioorg.Med Chem. Volume: 18 Pages: 8630-8641
  • Peer Reviewed
• [Presentation] 線虫の腸細胞オルガネラに局在するABC輸送体HAF-4とHAF-9の多量体形成と相互安定化 (2013)
  • Author(s): 丹治貴博 他
  • Organizer: 日本薬学会第133年会
  • Place of Presentation: 横浜
• [Presentation] 線虫腸細胞に存在するABC輸送体HAF-4・HAF-9陽性オルガネラの栄養環境に依存した変動 (2013)
  • Author(s): 黒田英介 他
  • Organizer: 日本薬学会第133年会
  • Place of Presentation: 横浜
• [Presentation] Age- and nutrition-dependent changes of intestinal granules in C.elegans加齢および栄養状態に依存した線虫腸内顆粒の変動 (2012)
  • Author(s): 丹治貴博、錦織健児、上田佑毅、黒田英介、青山玲子、遠山稿二郎、白石博久、大橋綾子
  • Organizer: 第6回先端医療薬学研究センター講演会
  • Place of Presentation: 岩手医科大学(矢巾)
  • Year and Date: 2012-03-16
• [Presentation] Proteomic and genetic approaches to the mechanisms underlying the formation of intestinal granules in C.elegansプロテオミクス及び遺伝学的アプローチによる線虫腸内顆粒の形成機構の解析 (2012)
  • Author(s): 錦織健児、丹治貴博、淺沼優美、及川嵩人、菊池里美、田邉麻莉、加茂政晴、石崎明、白石博久、大橋綾子
  • Organizer: 第6回先端医療薬学研究センター講演会
  • Place of Presentation: 岩手医科大学(矢巾)
  • Year and Date: 2012-03-16
• [Presentation] Identification of proteins affected by the deletion for haf-4and haf-9, the intestinal ABC transporter genes in Caenorhabditis elegans. (2012)
  • Author(s): Kenji Nishikoriet al
  • Organizer: New Frontiers of Metabolism Research in Biomedical Sciences
  • Place of Presentation: 東京
• [Presentation] Dynamic changes of major large intestinal granules in response to aging and starvation in Caenorhabditis elegans (2012)
  • Author(s): Hirohisa Shiraishi et al.
  • Organizer: New Frontiers of Metabolism Research in BiomedicalSciences
  • Place of Presentation: 東京
• [Presentation] Age-dependent alteration of major large intestinal granules in Caenorhabditis elegans. (2012)
  • Author(s): Ayako Ohashi-Kobayashi et al.
  • Organizer: Aging, Metabolism, Stress, Pathogenesis, and Small RNAs in C. elegans Topic Meeting
  • Place of Presentation: マディソン,米国
• [Presentation] Physical interaction of half ABC transporters HAF-4 and HAF-9, which are required for the biogenesis of the intestinal lysosome-related organelles in Caenorhabditis elegans. (2012)
  • Author(s): Takahiro Tanji et al.
  • Organizer: Aging, Metabolism, Stress, Pathogenesis, and Small RNAs in C. elegans Topic Meeting
  • Place of Presentation: マディソン,米国
• [Presentation] Age-dependent alteration of major large intestinal granules in Caenorhabditis elegans. (2012)
  • Author(s): Ohashi-Kobayashi A.
  • Organizer: Aging, Metabolism, Stress, Pathogenesis, and Small RNAs in C. elegans Topic Meeting
  • Place of Presentation: マディソン, 米国
• [Presentation] Physical interaction of half ABC transporters HAF-4 and HAF-9, which are required for the biogenesis of the intestinal lysosome-related organe (2012)
  • Author(s): Tanji T.
  • Organizer: Aging, Metabolism, Stress, Pathogenesis, and Small RNAs in C. elegans Topic Meeting
  • Place of Presentation: マディソン, 米国
• [Presentation] Dynamic changes of major large intestinal granules in response to aging and starvation in Caenorhabditis elegans. (2012)
  • Author(s): Shiraishi H.
  • Organizer: New Frontiers of Metabolism Research in Biomedical Sciences
  • Place of Presentation: 東京
• [Presentation] Identification of proteins affected by the deletion for haf-4 and haf-9, the intestinal ABC transporter genes in Caenorhabditis elegans. (2012)
  • Author(s): Nishikori K.
  • Organizer: New Frontiers of Metabolism Research in Biomedical Sciences
  • Place of Presentation: 東京
• [Presentation] 成虫期の線虫では加齢に伴った腸内顆粒のリニューアルが起こる (2011)
  • Author(s): 錦織健児、丹治貴博、白石博久、大橋綾子
  • Organizer: 第34回日本分子生物学会年会
  • Place of Presentation: パシフィコ横浜(横浜)
  • Year and Date: 2011-12-15
• [Presentation] Mutual stabilization of half-type ABC transporters HAF-4 and HAF-9 in Caenorhabditis elegans (2011)
  • Author(s): 丹治貴博、白石博久、錦織健児、前田正知、大橋綾子
  • Organizer: 第84回日本生化学会大会
  • Place of Presentation: 京都国際会議場(京都)
  • Year and Date: 2011-09-22
• [Presentation] プロテオミクスによる線虫(C.elegans)リソソーム関連ABC輸送体(HAF-4, HAF-9)の生理機能の解析 (2011)
  • Author(s): 錦織健児、丹治貴博、白石博久、大橋綾子
  • Organizer: 第6回トランスポーター研究会年会
  • Place of Presentation: 東北大学(仙台)
  • Year and Date: 2011-06-11
• [Presentation] 成虫期の線虫では加齢に伴った腸内顆粒のリニューアルが起こる (2011)
  • Author(s): 錦織健児 他
  • Organizer: 第34回日本分子生物学会年会
  • Place of Presentation: 横浜
• [Presentation] Mutual stabilization of half-type ABC transporters HAF-4 and HAF-9 in Caenorhabditis elegans. (2011)
  • Author(s): Takahiro Tanji et al.
  • Organizer: 第84回日本生化学会大会
  • Place of Presentation: 京都
• [Presentation] プロテオミクスによる線虫(C. elegans) リソソーム関連ABC輸送体(HAF-4, HAF-9)の生理機能の解析 (2011)
  • Author(s): 錦織健児 他
  • Organizer: 第6回トランスポーター研究会年会
  • Place of Presentation: 仙台
• [Presentation] Changes of protein profiles in the deletion mutant for haf-4 and haf-9, the intestinal ABC trapsporter genes in C.elegans (2010)
  • Author(s): Nishikori K, et al.
  • Organizer: BMB2010
  • Place of Presentation: 神戸
  • Year and Date: 2010-12-09
• [Presentation] Characterization of HAF-2 as a lysosomal ABC transporter in Caenorhabditis elegans. (2010)
  • Author(s): Shiraishi H, et al.
  • Organizer: East Asia Worm Meeting 2010
  • Place of Presentation: 東京
  • Year and Date: 2010-07-12
• [Presentation] Genetic interaction of HAF-4 and HAF-9, half-type ABC transporters required for the biogenesis of intestinal lysosome-related organelle in Caenorhabditis elegans. (2010)
  • Author(s): Tanji T, et al.
  • Organizer: East Asia Worm Meeting 2010
  • Place of Presentation: 東京
  • Year and Date: 2010-07-12
• [Presentation] ATP-binding cassette transporters HAF-4 and HAF-9, homologues of human lysosomal peptide transporter TAP-like are required for normal formation of a subset of intestinal granules in Caenorhabditis elegans. (2010)
  • Author(s): Nishikori K, et al.
  • Organizer: East Asia Worm Meeting 2010
  • Place of Presentation: 東京
  • Year and Date: 2010-07-11
• [Presentation] Genetic and physical interactions between half-type ABC transporters HAF-4 and HAF-9, which are required for the biogenesis of intestinal lysosome-related organelle in Caenorhabditis elegans. (2010)
  • Author(s): Takahiro Tanji et al
  • Organizer: BMB2010
  • Place of Presentation: 神戸
• [Presentation] Changes of protein profiles in the deletion mutant for haf-4 and haf-9, the intestinal ABC transporter genes in C. elegans. (2010)
  • Author(s): Kenji Nishikori et al
  • Organizer: BMB2010
  • Place of Presentation: 神戸
• [Presentation] ATP-binding cassette transporters HAF-4 and HAF-9, homologues of human lysosomal peptide transporter TAP-like are required for biogenesis of a subset of intestinal granules in Caenorhabditis elegans. (2010)
  • Author(s): Kenji Nishikori et al
  • Organizer: 4th East Asia C. elegans Meeting
  • Place of Presentation: 東京
• [Presentation] Genetic interaction of HAF-4 and HAF-9, half-type ABC transporters required for the biogenesis of intestinal lysosome-related organelle in Caenorhabditis elegans. (2010)
  • Author(s): Takahiro Tanji et al
  • Organizer: 4th East Asia C. elegans Meeting
  • Place of Presentation: 東京
• [Presentation] Characterization of HAF-2 as a lysosomal ABC transporter in Caenorhabditis elegans. (2010)
  • Author(s): Hirohisa Shiraishi et al
  • Organizer: 4th East Asia C. elegans Meeting
  • Place of Presentation: 東京
• [Book] 生物学大辞典 (2010)
  • Author(s): 大橋綾子(分担執筆)
  • Publisher: 東京化学同人
• [Remarks]
  • URL: http://gaia-sb.iwate-med.ac.jp/pharm/?page_id=42
• [Remarks] 岩手医科大学薬学部生体防御学講座HP
  • URL: http://gaia-sb.iwate-med.ac.jp/pharm/?page_id=42