| Project/Area Number |
22590074
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Biological pharmacy
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| Research Institution | Hoshi University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
TAKAHASHI Katsuhiko 星薬科大学, 薬学部, 准教授 (80307066)
今井 正彦 星薬科大学, 薬学部, 助教 (40507670)
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| Co-Investigator(Renkei-kenkyūsha) |
IMAI Masahiko 星薬科大学, 薬学部, 助教 (40507670)
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| Project Period (FY) |
2010 – 2012
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| Project Status |
Completed (Fiscal Year 2012)
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| Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2012: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2011: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2010: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
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| Keywords | シグナル伝達 / 発現制御 / 蛋白質修飾 / プロテインキナーゼ A / レチノイル化 / レチノイン酸 / プロテインキナーゼA |
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| Research Abstract |
Vitamin A is a micronutrient that is indispensable to maintaining health. The free acid (retinoic acid, RA) is an active form of vitamin A that elicits a wide variety of actions which are important to the body. It has attracted considerable attention from the viewpoint of prevention and treatment of cancers and neurological diseases etc. In the current study, retinoylation (protein post-translational modification by RA) was found to be a new RA mechanism of action that is not dependent on nuclear receptors. These findings resulted from detailed investigations using the human promyelocytic leukemia cell line HL60, which is differentiated by RA. New signal transmission mechanisms were analyzed that resulted in the identification of new RA-binding target proteins in the nucleus as well as proteins phosphorylated by retinoylated nuclear protein kinase A. These studies have clarified protein modifications that are associated with the actions of vitamin A.
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