Elucidation of the mechanism of nuclear signal transduction by retinoic acid based on new insight and its applications
Project/Area Number |
22590074
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Single-year Grants |
Section | 一般 |
Research Field |
Biological pharmacy
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Research Institution | Hoshi University |
Principal Investigator |
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Co-Investigator(Kenkyū-buntansha) |
TAKAHASHI Katsuhiko 星薬科大学, 薬学部, 准教授 (80307066)
今井 正彦 星薬科大学, 薬学部, 助教 (40507670)
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Co-Investigator(Renkei-kenkyūsha) |
IMAI Masahiko 星薬科大学, 薬学部, 助教 (40507670)
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Project Period (FY) |
2010 – 2012
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Project Status |
Completed (Fiscal Year 2012)
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Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2012: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2011: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2010: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
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Keywords | シグナル伝達 / 発現制御 / 蛋白質修飾 / プロテインキナーゼ A / レチノイル化 / レチノイン酸 / プロテインキナーゼA |
Research Abstract |
Vitamin A is a micronutrient that is indispensable to maintaining health. The free acid (retinoic acid, RA) is an active form of vitamin A that elicits a wide variety of actions which are important to the body. It has attracted considerable attention from the viewpoint of prevention and treatment of cancers and neurological diseases etc. In the current study, retinoylation (protein post-translational modification by RA) was found to be a new RA mechanism of action that is not dependent on nuclear receptors. These findings resulted from detailed investigations using the human promyelocytic leukemia cell line HL60, which is differentiated by RA. New signal transmission mechanisms were analyzed that resulted in the identification of new RA-binding target proteins in the nucleus as well as proteins phosphorylated by retinoylated nuclear protein kinase A. These studies have clarified protein modifications that are associated with the actions of vitamin A.
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Report
(4 results)
Research Products
(83 results)
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[Journal Article] Acetoacetyl-CoA synthetase is essential for normal neuronal development2012
Author(s)
HASEGAWA, S., KUME, H., IINUMA, S., YAMASAKI, M., TAKAHASHI, N., ANDFUKUI, T.
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Journal Title
Biochem. Biophys. Res. Commun.
Volume: 427(2)
Pages: 398-403
Related Report
Peer Reviewed
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[Journal Article] Growth inhibition of a human colon carcinoma cell, COLO 201, by a natural product, Vitex agnus-castus fruits extract, in vivo and in vitro2012
Author(s)
IMAI, M., YUAN, B., KIKUCHI, H., SAITO, M., OHYAMA, K., HIROBE, C., OSHIMA, T., HOSOYA, T., MORITA, H., and TOYODA H.
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Journal Title
Adv. Biol. Chem.
Volume: 2
Pages: 20-28
Related Report
Peer Reviewed
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[Journal Article] Covalent binding of retinoic acid to cytokeratins 16 and 10 accompanies its action in mouse skin tissues2011
Author(s)
TAKAHASHI, N., OHBA, T., IMAI, M., SASAKI, Y., KUMAOKA, T., TAKAHASHI K. AND FUJIU, Y.
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Journal Title
FASEB J
Volume: 25(6)
Pages: 951-955
Related Report
Peer Reviewed
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[Journal Article] Enhanced growth inhibitory effect of 5-fluorouracil in combination with Vitex agnus-castus fruits extract against a human colon adenocarcinoma cell line, COLO 2012011
Author(s)
Imai, M., Kikuchi, H., Yuan, B., Aihara, Y., Mizokuchi, A., Ohyama, K., Hirobe, C., Toyoda, H.
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Journal Title
J.Chin.Clin.Med.
Volume: 6
Pages: 14-19
Related Report
Peer Reviewed
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[Journal Article] Rab3a and Rab27b Expression in Nonfunctioning Pituitary Adenomas2011
Author(s)
Sasaki, A., Takahashi, K., Yamamoto, G., Tsunoda, Y., Abe, T., Tachikawa, T.
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Journal Title
Showa Univ.J.Med.Sci
Volume: 22
Pages: 183-192
NAID
Related Report
Peer Reviewed
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[Presentation] A new anti-tumor derivative of N-(4-hydroxyphenyl)retinamid (fenretinide) that potently suppresses proliferation through apoptosis in human neuroblastoma NB-39-nu cells, FASEB Scientific Research conference "Retinoids"2012
Author(s)
Noriko Takahashi^a, Rumi Egawa^a, Masahiko Imai^a, Katsuhiko Takahashi^a, and Masue Imaizumi^b
Organizer
2012, Silvertree, Snowmass Village, Colorado, USA, a^Hoshi University
Place of Presentation
b^Miyagi Children's Hospital
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