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Elucidation of activation mechanism of human acetyl-CoA carboxylase

Research Project

Project/Area Number 22590113
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Drug development chemistry
Research InstitutionThe Institute of Physical and Chemical Research

Principal Investigator

KUNISHIMA Naoki  独立行政法人理化学研究所, タンパク質結晶構造解析研究グループ, グループ副ディレクター (70415149)

Co-Investigator(Renkei-kenkyūsha) MATSUURA Yoshinori  独立行政法人理化学研究所, タンパク質結晶構造解析研究グループ, リサーチアソシエイト (50513462)
Project Period (FY) 2010 – 2012
Project Status Completed (Fiscal Year 2012)
Budget Amount *help
¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000)
Fiscal Year 2012: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2011: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2010: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Keywords生活習慣病 / 脂肪酸生合成 / 結晶構造解析 / X線回折 / 酵素 / タンパク質 / 立体構造 / 薬物設計
Research Abstract

Toward the elucidation of the activation mechanism of acetyl-CoA carboxylase (ACC) that catalyzes the rate-limiting step of the fatty-acid biosynthesis, we tried the protein crystallography of the central domain of human ACC (hBCCP1, hBCCP2) and its activation enzyme biotin protein ligase (hBPL). The genes were synthesized for a large-scale expression using E.coli. These proteins were generally expressed as inclusion bodies that could not be solubilized by methods including the denaturation/refolding and the substitution of cysteine with alanine.

Report

(4 results)
  • 2012 Annual Research Report   Final Research Report ( PDF )
  • 2011 Annual Research Report
  • 2010 Annual Research Report
  • Research Products

    (4 results)

All Other

All Remarks (4 results)

  • [Remarks]

    • URL

      http://www.spring8.or.jp/ja/news_publications/press_release/2008/080327

    • Related Report
      2012 Final Research Report
  • [Remarks] 生活習慣病に関連するタンパク質複合体の結晶構造を世界で初めて決定

    • URL

      http://www.spring8.or.jp/ja/news_publications/press_release/2008/080327

    • Related Report
      2012 Annual Research Report
  • [Remarks]

    • URL

      http://www.spring8.or.jp/ia/news_publications/press_release/2008/080327

    • Related Report
      2011 Annual Research Report
  • [Remarks]

    • URL

      http://www.spring8.or.jp/Ja/news_publications/press_release/2008/080327

    • Related Report
      2010 Annual Research Report

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Published: 2010-08-23   Modified: 2019-07-29  

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