Analysis of the mechanism of immunological memory against the capsular polysaccharides of Streptococcus pneumoniae

• Project/Area Number: 22590115
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Environmental pharmacy
• Research Institution: Hoshi University
• Principal Investigator: TSUIJI Makoto 星薬科大学, 薬学部, 准教授 (90302611)
• Project Period (FY): 2010 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000); Fiscal Year 2012: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2011: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2010: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
• Keywords: 微生物 / 感染症学 / 免疫記憶 / ワクチン / 記憶B細胞 / 肺炎球菌 / 莢膜糖鎖

Research Abstract

Streptococcus pneumoniae is a common pathogen of pneumonia among children and elderly persons. To identify the population of B cells, which have an important role of inducing the primary responses and memory responses against pneumococcal polysaccharides, the pneumococcal polysaccharides of serotype 3 (PPS3) were injected into the peritoneal cavity of mice. We found that expansion of polyreactive IgM+ B cell clones were detected in spleen on day 3, afterward specific IgM+ B cell clones were selected on day 7. It suggests that PPS3 is thought as TI type 1 antigens during the primary phase.

Research Products

• [Journal Article] Involvement of transcription factor Ets-1 in the expression of the α3 integrin subunit gene. (2012)
  • Author(s): Kamoshida G, Matsuda A, Katabami K, Kato T, Mizuno H, Sekine W, Oku T, Itoh S, Tsuiji M, Hattori Y, Maitani Y, Tsuji T.
  • Journal Title: FEBS J. Volume: 279(24) Issue: 24 Pages: 4535-46
  • DOI: 10.1111/febs.12040
  • Peer Reviewed
• [Journal Article] Constitutive turnover of phosphorylation at Thr-412 of human p57/coronin-1 regulates the interaction with actin. (2012)
  • Author(s): Oku T, Nakano M, Kaneko Y, Ando Y, Kenmotsu H, Itoh S, Tsuiji M, Seyama Y, Toyoshima S ,Tsuji T.
  • Journal Title: J Biol Chem. Volume: 287(51) Issue: 51 Pages: 42910-20
  • DOI: 10.1074/jbc.m112.349829
  • Peer Reviewed
• [Journal Article] Role of central leptin signaling in the starvation-induced alteration of B cell development (2011)
  • Author(s): M. Tanaka, T. Suganami, M. Kim-Saijo, C. Toda, M. Tsuiji, K. Ochi, Y. Kamei, Y. Minokoshi, Y. Ogawa
  • Journal Title: J. Neurosci. Volume: 31 Issue: 23 Pages: 8373-8380
  • DOI: 10.1523/jneurosci.6562-10.2011
  • Peer Reviewed
• [Journal Article] Distribution and function of macrophage galactose-type C-type lectin 2 (MGL2/CD301b): efficient uptake and presentation of glycosylated antigens by dendritic cells. (2010)
  • Author(s): Denda-Nagai K, Aida S, Saba K, Suzuki K, Moriyama S, Oo-Puthinan S, Tsuiji M, Morikawa A, Kumamoto Y, Sugiura D, Kudo A, Akimoto Y, Kawakami H, Bovin NV, Irimura T.
  • Journal Title: J Biol Chem. Volume: 285(25) Issue: 25 Pages: 19193-204
  • DOI: 10.1074/jbc.m110.113613
  • Peer Reviewed
• [Journal Article] Distribution and function of macrophage galactose-type C-type lectin 2 (MGL2/CD301b) : efficient uptake and presentation of glycosylated antigens by dendritic cells (2010)
  • Author(s): Denda-Nagai K, Aida S, Saba K, Suzuki K, Moriyama S, Oo-Puthinan S, Tsuiji M, Morikawa A, Kumamoto Y, Sugiura D, Kudo A, Akimoto Y, Kawakami H, Bovin NV, Irimura T
  • Journal Title: Journal of Biological Chemistry Volume: 285(25) Pages: 19193-19204
  • Peer Reviewed
• [Presentation] 免疫細胞活性化マーカーCD83 のリガンド探索 (2012)
  • Author(s): 安藤祐介,栗坂知里,奥輝明,築地信,辻勉.
  • Organizer: 第35回日本分子生物学会年会
  • Year and Date: 2012-12-12
• [Presentation] Analysis of the immune resonse against capsular polysaccharides of Streptococcus pneumoniae and the ligands of CD83, a candidate for sugar-recognizing molecule. (2012)
  • Author(s): Tsuiji M, Ando Y, Tanaka M, Imai N, Hayashizaki K, Kurisaka C, Oku T, Tsubata T, Tsuji T.
  • Organizer: International Symposium on Glyco-minded Biology of Diseases as a Basis of Pharmaceutical Sciences
  • Year and Date: 2012-12-01
• [Presentation] 鍔田武志,Michel C. Nussenzweig,辻勉. 末梢血記憶 B 細胞抗体 遺伝子レパトローム解析 (2012)
  • Author(s): 築地信,安藤祐介
  • Organizer: 第13回Pharmaco-Hematology シンポジウム
  • Year and Date: 2012-06-15
• [Presentation] 末梢血記憶B細胞抗体遺伝子レパトローム解析 (2012)
  • Author(s): 築地信、安藤祐介、鍔田武志、Michel C. Nussenzweig、辻勉
  • Organizer: 第１３回Pharmaco-Hematologyシンポジウム
  • Place of Presentation: 東京
• [Presentation] Analysis of the immune response against capsular polysaccharides of Streptococcus pneumonia and the ligands of CD83, a candidate for sugar-recognizing molecule (2012)
  • Author(s): Tsuiji M, Ando Y, Tanaka M, Imai N, Hayashizaki K, Kurisaka C, Oku T, Tsubata T, Tsuji T.
  • Organizer: International Symposium on Glyco-minded Biology of Diseases as a Basis of Pharmaceutical Sciences
  • Place of Presentation: Tokyo
• [Presentation] 免疫細胞活性化マーカーCD83のリガンド探索 (2012)
  • Author(s): 安藤祐介、栗坂知里、奥輝明、築地信、辻勉
  • Organizer: 第３５回日本分子生物学会年会
  • Place of Presentation: 福岡
• [Presentation] The immune response during the primary phase against capsular polysaccharides of Streptococcus pneumoniae. (2010)
  • Author(s): Tanaka M, Imai N ,Tsubata T, Tsuiji M.
  • Organizer: 14^<th> International congress of Immunology, Aug. 25^<th>
  • Year and Date: 2010-08-25
• [Presentation] The immune response during the primary phase against capsular polys accharides of Streptococcus pneumoniae. (2010)
  • Author(s): Tanaka M, Imai N, Tsubata T, Tsuiji M
  • Organizer: 14th International congress of Immunology
  • Place of Presentation: Kobe Convention Center, Kobe, Japan
  • Year and Date: 2010-08-25
• [Presentation] The immune response during the primary phase against capsular polysaccharides of Streptococcus pneumoniae. (2010)
  • Author(s): Tsuiji M, Tanaka M, Imai N, Hayashizaki K, Tsubata T.
  • Organizer: The 28^<th> Naito conference on Glycan Expression and Regulation
  • Year and Date: 2010-07-28
• [Presentation] The immune response during the primary phase against capsular polys accharides of Streptococcus pneumoniae. (2010)
  • Author(s): Tsuiji M, Tanaka M, Imai N, Hayashizaki K, Tsubata T.
  • Organizer: The 28th Naito conference on Glycan Expression and Regulation [1]
  • Place of Presentation: Shonan Village Center, Kanagawa, Japan
  • Year and Date: 2010-07-28
• [Remarks]
  • URL: http://www.hoshi.ac.jp/home/kyoiku/kenkyusosiki.html