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Lipid hydroperoxide-derived modifications to angiotensin peptides:a novel approach for cardiovascular disease

Research Project

Project/Area Number 22590130
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Medical pharmacy
Research InstitutionTohoku University

Principal Investigator

LEE Seon hwa  東北大学, 大学院・薬学研究科, 助教 (60519776)

Co-Investigator(Kenkyū-buntansha) OE Tomoyuki  東北大学, 大学院・薬学研究科, 教授 (10203712)
GOTO Takaaki  東北大学, 大学院・薬学研究科, 講師 (40344684)
Project Period (FY) 2010 – 2012
Project Status Completed (Fiscal Year 2012)
Budget Amount *help
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2012: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2011: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2010: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Keywords臨床化学 / 酸化ストレス / 化学修飾 / アンジオテンシン / oxidative stress / lipid peroxidation / angiotensin II / 4-oxo-2(E)-nonenal / 4-hydroxy-2(E)-nonenal / mass spectrometry / lipid hydroperoxide
Research Abstract

In the reactions of angiotensin (Ang) II with lipid peroxidation-derived bifunctional electrophiles, 4-oxo-2(E)-nonenal (ONE) or 4-hydroxy-2(E)-nonenal (HNE), the major modifications have been identified at the N-terminus, Asp^1, Arg^2, and His^6 of Ang II. The identities of ONE- and HNE-modified Ang IIs were confirmed by mass spectrometry (MS) before and after the reaction with sodium borohydride. A novel ONE-derive pyruvamide-Ang II was formed via oxidative decarboxylation of N-terminal aspartic acid. The unexpected formations of Arg- and His- modifications were confirmed using model reactions with N^α-tert-butoxycarbonyl-amino acids. To investigate the biological effects of modified Ang IIs, MS-based label-free Ang II type 1 (AT_1) receptor binding assay and aminopeptidase (AP) A enzyme assay have been developed. Using these methodologies, we have confirmed that the oxidative modifications on the N-terminus of Ang II disrupt interactions with AT_1 receptor and APA, which could affect the regulation of cardiovascular function.

Report

(4 results)
  • 2012 Annual Research Report   Final Research Report ( PDF )
  • 2011 Annual Research Report
  • 2010 Annual Research Report
  • Research Products

    (66 results)

All 2013 2012 2011 2010 Other

All Journal Article (20 results) (of which Peer Reviewed: 20 results) Presentation (40 results) (of which Invited: 5 results) Book (2 results) Remarks (4 results)

  • [Journal Article] Can Edman degradation be used for quantification? Isotope-dilution liquid chromatography-electrospray ionization tandem mass spectrometry and the long-term stability of 20 phenylthiohydantoin-amino acids2013

    • Author(s)
      Satoh, R., Goto, T., Lee, S.H., Oe, T.
    • Journal Title

      Anal. Bioanal. Chem

      Volume: (in press) Issue: 25 Pages: 8001-8010

    • DOI

      10.1007/s00216-013-6901-6

    • Related Report
      2012 Annual Research Report 2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] MALDI-TOF/MS-based label-free binding assay for angiotensin II type1 receptor: application for novel angiotensin peptides2013

    • Author(s)
      Lee, S.H., Masuda, T., Goto, T., Oe, T.
    • Journal Title

      Anal. Biochem

      Volume: 437 Issue: 1 Pages: 10-16

    • DOI

      10.1016/j.ab.2013.01.023

    • Related Report
      2012 Annual Research Report 2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Chemical modificomics: a novel strategy forefficient biomarker discovery through chemical modifications on a target peptide or protein2012

    • Author(s)
      Goto, T., Kojima, S., Shitamichi, S., Lee, S.H., Oe, T.
    • Journal Title

      Anal. Methods

      Volume: 4 Issue: 7 Pages: 1945-1952

    • DOI

      10.1039/c2ay05841c

    • Related Report
      2012 Annual Research Report 2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Non-invasive proteomic analysis of human skin keratins: Screening of methionine oxidation in keratins by mass spectrometry2011

    • Author(s)
      Lee, S.H., Miyamoto, K., Goto, T., Oe, T.
    • Journal Title

      J. Proteomics

      Volume: 75 Issue: 2 Pages: 435-449

    • DOI

      10.1016/j.jprot.2011.08.010

    • Related Report
      2012 Final Research Report 2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Synthesis of deuterium labeled analogs of 4-oxo-2(E)-nonenal2011

    • Author(s)
      Arora, J. S., Oe, T., Blair, I. A.
    • Journal Title

      J. Labelled Compd. Radiopharm

      Volume: 54 Issue: 5 Pages: 247-251

    • DOI

      10.1002/jlcr.1860

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Mass spectrometric characterization of modification to angiotensin II by lipid peroxidation products, 4-oxo-2(E)-nonenal and4-hydroxy-2(E)-nonenal2010

    • Author(s)
      Lee, S.H., Takahashi, R., Goto, T., Oe, T.
    • Journal Title

      Chem. Res. Toxicol

      Volume: 23 Issue: 11 Pages: 1771-1785

    • DOI

      10.1021/tx100228q

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Combining [^<13>C_6]-phenylisothiocyanate and the Edman degradation reaction: a possible breakthrough for absolute quantitative proteomics together with protein identification2010

    • Author(s)
      Oe, T., Maekawa, M., Satoh, R., Lee, S.H., and Goto, T.
    • Journal Title

      Rapid Commun. Mass Spectrom

      Volume: 24 Issue: 2 Pages: 173-179

    • DOI

      10.1002/rcm.4372

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Analysis of epoxyeicosatrienoic acids by chiral liquid chromatography/ electron capture atmospheric pressure chemical ionization mass spectrometry using [^<13>C]-analog internal standards2010

    • Author(s)
      Mesaros, C., Lee, S.H., Blair, I.A.
    • Journal Title

      Rapid Commun. Mass Spectrom

      Volume: 24 Issue: 22 Pages: 3237-3247

    • DOI

      10.1002/rcm.4760

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Liquid chromatography-mass spectrometry (LC-MS) of steroid hormone metabolitesand its applications2010

    • Author(s)
      Penning, T.M., Lee, S.H., Jin, Y., Gutierrez, A., Blair, I.A.
    • Journal Title

      J. Steroid Biochem. Mol. Biol

      Volume: 121 Issue: 3-5 Pages: 546-555

    • DOI

      10.1016/j.jsbmb.2010.01.005

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] A simple and efficient approach to improve protein identification by the peptide mass fingerprinting method: Concomitant use of negative ionization2010

    • Author(s)
      Sanaki, T., Suzuki, M., Lee, S.H., Goto, T., Oe, T.
    • Journal Title

      Anal. Methods

      Volume: 2 Issue: 8 Pages: 1144-1151

    • DOI

      10.1039/c0ay00215a

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Aldo-keto reductase 1C3 expression in MCF-7 cells reveals roles in steroid hormone and prostaglandin metabolism that mayexplain its over-expression in breast cancer2010

    • Author(s)
      Byrns, M.C., Duan, L., Lee, S.H., Blair, I.A., Penning, T.M.
    • Journal Title

      J. Steroid Biochem. Mol. Biol

      Volume: 118 Issue: 3 Pages: 177-187

    • DOI

      10.1016/j.jsbmb.2009.12.009

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] A 13-oxo-9,10- epoxytridecenoate phospholipid analogue of the genotoxic 4,5-epoxy-2(E)-decenal: detection in vivo, chemical synthesis, and adduction with DNA2010

    • Author(s)
      Mesaros, C., Gugiu, B.G., Zhou, R., Lee, S.H., Choi, J., Laird, J., Blair, I.A., Salomon, R.G.
    • Journal Title

      Chem. Res. Toxicol

      Volume: 23 Issue: 3 Pages: 516-27

    • DOI

      10.1021/tx9002484

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Major biliary bile acids of the Medaka (Oryzias latipes): 25R- and 25S- epimers of 3alpha,7alpha,12alpha- trihydroxy-5beta-cholestanoic acid2010

    • Author(s)
      Hagey, L. R., Lida, T., Tamegai, H., Ogawa, S., Une, M., Asahina, K., Mushiake, K., Goto, T., Mano, N., Goto, J., Krasowski, M. D., Hofmann, A. F.
    • Journal Title

      Zoolog. Sci

      Volume: 27 Issue: 7 Pages: 565-573

    • DOI

      10.2108/zsj.27.565

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Mass spectrometric characterization of modifications to angiotensin II by lipid peroxidation products, 4-oxo-2(E)-nonenal and 4-hydroxy-2(E)-nonenal2010

    • Author(s)
      Seon Hwa Lee, Ryo Takahashi, Takaaki Goto, Tomoyuki Oe
    • Journal Title

      Chemical Research in Toxicology

      Volume: Vol.23 Pages: 1771-1785

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Analysis of epoxyeicosatrienoic acids by chiral liquid chromatography/electron capture atmospheric pressure chemical ionization mass spectrometry using [^<13>C]-analog internal standards2010

    • Author(s)
      Clementina Mesaros, Seon Hwa Lee, Ian A.Blair
    • Journal Title

      Rapid Communications in Mass Spectrometry

      Volume: Vol.24 Pages: 3237-3247

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] A simple and efficient approach to improve protein identification by the peptide mass fingerprinting method : concomitant use of negative ionization2010

    • Author(s)
      Takao Sanaki, Mao Suzuki, Seon Hwa Lee, Takaaki Goto, Tomoyuki Oe
    • Journal Title

      Analytical Methods

      Volume: Vol.2 Pages: 1144-1151

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Liquid chromatography-mass spectrometry (LC-MS) of steroid hormone metabolites and its applications2010

    • Author(s)
      Trevor M.Penning, Seon Hwa Lee, Yi Jin, Alejandro Gutierrez, Ian A.Blair
    • Journal Title

      Journal of Steroid Biochemistry and Molecular Biology

      Volume: Vol.121 Pages: 546-555

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Combining [^<13>C_6]-phenylisothiocyanate and the Edman degradation reaction : a possible breakthrough for absolute quantitative proteomics together with protein identification2010

    • Author(s)
      Tomoyuki Oe, Masamitsu Maekawa, Ryo Satoh, Seon Hwa Lee, Takaaki Goto
    • Journal Title

      Rapid Communications in Mass Spectrometry

      Volume: Vol.24 Pages: 173-179

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] A 13-oxo-9,10-epoxytridecenoate phospholipid analogue of the genotoxic 4,5-epoxy-2(E)-decenal : detection in vivo, chemical synthesis, and adduction with DNA2010

    • Author(s)
      Clementina Mesaros, Bogdan G.Gugiu, Rong Zhou, Seon Hwa Lee, Jaewoo Choi, James Laird, Ian A.Blair, Robert G.Salomon
    • Journal Title

      Chemical Research in Toxicology

      Volume: Vol.23 Pages: 516-527

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Aldo-keto reductase 1C3 expression in MCF-7 cells reveals roles in steroid hormone and prostaglandin metabolism that may explain its over-expression in breast cancer2010

    • Author(s)
      Michael C.Byrns, Ling Duan, Seon Hwa Lee, Ian A.Blair, Trevor M.Penning
    • Journal Title

      Journal of Steroid Biochemistry and Molecular Biology

      Volume: Vol.118 Pages: 177-187

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Presentation] 酸化ストレスによる生体高分子の化学修飾機構に関する研究。日本薬学東北支部2012

    • Author(s)
      Lee, S.H.
    • Organizer
      第34回東北薬学セミナー
    • Place of Presentation
      仙台
    • Year and Date
      2012-12-01
    • Related Report
      2012 Final Research Report
  • [Presentation] 化学修飾スクリーニングを目的とした修飾ペプチドのフラグメンテーション解析2012

    • Author(s)
      吉泉玲央奈、李宣和、後藤貴章、大江知行
    • Organizer
      第51回日本薬学会東北支部大会
    • Place of Presentation
      青森
    • Year and Date
      2012-10-07
    • Related Report
      2012 Final Research Report
  • [Presentation] バイオマーカー探索を指向した化学修飾オミクス2012

    • Author(s)
      大江知行
    • Organizer
      第52回日本臨床化学会年次学術集会
    • Place of Presentation
      盛岡
    • Year and Date
      2012-09-08
    • Related Report
      2012 Final Research Report
  • [Presentation] 非放射性レセプターアッセイによる酸化修飾アンジオテンシンIIとAT1レセプター結合能の評価2012

    • Author(s)
      増田拓朗、李宣和、後藤貴章、大江知行
    • Organizer
      みちのく分析科学シンポジウム2012
    • Place of Presentation
      米沢
    • Year and Date
      2012-07-21
    • Related Report
      2012 Final Research Report
  • [Presentation] Pyridoxamine-mediated transamination of pyruvamide- angiotensin II, a novel angiotensin peptide derived from 4-oxo-2(E)- nonenal2012

    • Author(s)
      景賢淑、李宣和、後藤貴章、大江知 行
    • Organizer
      みちのく分析科学シンポジウム2012
    • Place of Presentation
      米沢
    • Year and Date
      2012-07-21
    • Related Report
      2012 Final Research Report
  • [Presentation] 効率的バイオマーカー探索基盤としての『化学修飾オミクス』2012

    • Author(s)
      大江知行
    • Organizer
      第13回機能構造と分析化学シンポジウム
    • Place of Presentation
      仙台
    • Year and Date
      2012-02-18
    • Related Report
      2012 Final Research Report
  • [Presentation] Novel lipid hydroperoxide- derived oxidized angiotensin peptides as potential biomarkers of cardiovascular disease2012

    • Author(s)
      Lee, S.H.
    • Organizer
      Sendai Symposium on Analytical Sciences
    • Place of Presentation
      Sendai, Japan
    • Related Report
      2012 Final Research Report
  • [Presentation] Chemical modificomics: alternative analytical platform for efficient biomarker discovery2012

    • Author(s)
      Oe, T.
    • Organizer
      Sendai Symposium on Analytical Sciences
    • Place of Presentation
      Sendai, Japan
    • Related Report
      2012 Final Research Report
  • [Presentation] Targeted metabolomic analysis of modified angiotensin peptides as potential biomarkers of cardiovascular diseases2012

    • Author(s)
      Lee, S.H.
    • Organizer
      The 60th Annual American Society for Mass Spectrometry (ASMS) Conference
    • Place of Presentation
      Vancouver, Canada
    • Related Report
      2012 Final Research Report
  • [Presentation] LC-ESI/MS analysis of protein modification derived from a mixture of13-HPODE and [^<13>C_<18>]-13-HPODE2012

    • Author(s)
      Takahashi, R., Lee, S.H., Goto, T., Oe, T.
    • Organizer
      19th International Mass Spectrometry Conference
    • Place of Presentation
      Kyoto
    • Related Report
      2012 Final Research Report
  • [Presentation] 過酸化脂質分解物4-hydroperoxy-2(E)-nonenalによるangiotensin II修飾反応の解析2012

    • Author(s)
      高橋亮、李宣和、後藤貴章、大江知行
    • Organizer
      日本薬学会第132年会
    • Place of Presentation
      札幌
    • Related Report
      2012 Final Research Report 2011 Annual Research Report
  • [Presentation] Novel lipid hydroperoxide- derived oxidized angiotensin peptides as potential biomarkers of cardiovascular disease.2012

    • Author(s)
      Lee, S.H.
    • Organizer
      Sendai Symposium on Analytical Sciences
    • Place of Presentation
      Sendai
    • Related Report
      2012 Annual Research Report
    • Invited
  • [Presentation] Chemical modificomics: alternative analytical platform for efficient biomarker discovery.2012

    • Author(s)
      Oe, T.
    • Organizer
      Sendai Symposium on Analytical Sciences
    • Place of Presentation
      Sendai
    • Related Report
      2012 Annual Research Report
    • Invited
  • [Presentation] Targeted metabolomic analysis of modified angiotensin peptides as potential biomarkers of cardiovascular diseases.2012

    • Author(s)
      Lee, S.H., Takahashi, R., Kojima, S., Suzuki, S., Goto, T., Oe, T.
    • Organizer
      The 60th Annual American Society for Mass Spectrometry(ASMS) Conference
    • Place of Presentation
      Vancouver, Canada
    • Related Report
      2012 Annual Research Report
    • Invited
  • [Presentation] LC-ESI/MS analysis of protein modification derived from a mixture of 13-HPODE and [13C18]-13-HPODE.2012

    • Author(s)
      Takahashi, R., Lee, S. H., Goto, T., Oe, T.
    • Organizer
      19th International Mass Spectrometry Conferrence
    • Place of Presentation
      Kyoto
    • Related Report
      2012 Annual Research Report
  • [Presentation] Chemical modificomics: alternative analytical platform for efficient biomarker discovery2011

    • Author(s)
      Oe, T.
    • Organizer
      International Symposium on Recent Advances in Drug Discovery and Development
    • Place of Presentation
      Chongju, Korea
    • Year and Date
      2011-11-18
    • Related Report
      2012 Final Research Report
  • [Presentation] 過酸化脂質由来の反応性アルデヒドによるアンジオテンシンIIの化学修飾解析2011

    • Author(s)
      高橋亮、李宣和、後藤貴章、大江知行
    • Organizer
      みちのく分析科学シンポジウム2011
    • Place of Presentation
      山形
    • Year and Date
      2011-07-23
    • Related Report
      2011 Annual Research Report
  • [Presentation] Targeted metabolomic approach for cardiovascular diseases2011

    • Author(s)
      Lee, S.H.
    • Organizer
      Fall International Convention of the Pharmaceutical Society of Korea
    • Place of Presentation
      Incheon, Korea
    • Related Report
      2012 Final Research Report
  • [Presentation] MALDI-TOF/MSを用いた非放射性レセプターアッセイ法の開発研究:酸化修飾アンジオテンシンIIとAT1レセプターの結合能の評価2011

    • Author(s)
      増田拓朗、李宣和、後藤貴章、大江知行
    • Organizer
      第50回日本薬学会東北支部大会
    • Place of Presentation
      仙台
    • Related Report
      2012 Final Research Report 2011 Annual Research Report
  • [Presentation] イオン化2011

    • Author(s)
      大江知行
    • Organizer
      The 38thBiological Mass Spectrometry Conference (BMS2011)
    • Place of Presentation
      箱根町
    • Related Report
      2012 Final Research Report
  • [Presentation] Targeted metabolomic approach for cardiovascular diseases2011

    • Author(s)
      Seon Hwa Lee
    • Organizer
      Fall International Convention of the Pharmaceutical Society of Korea
    • Place of Presentation
      Incheon, Korea(招待講演)
    • Related Report
      2011 Annual Research Report
  • [Presentation] LC/ESI-MS/MSを用いた血中アンジオテンシン一斉定量法の検討2011

    • Author(s)
      鈴木深作、後藤貴章、李宣和、大江知行
    • Organizer
      第50回日本薬学会東北支部大会
    • Place of Presentation
      仙台
    • Related Report
      2011 Annual Research Report
  • [Presentation] LC/ESI-MSによるアンジオテンシンIIの過酸化脂質修飾解析2011

    • Author(s)
      高橋亮、李宣和、後藤貴章、大江知行
    • Organizer
      第22回クロマトグラフィー科学会議
    • Place of Presentation
      仙台
    • Related Report
      2011 Annual Research Report
  • [Presentation] ELISA用抗体の交差反応性を利用した化学修飾オミクス2010

    • Author(s)
      大江知行
    • Organizer
      日本分析化学会第59年会
    • Place of Presentation
      仙台
    • Year and Date
      2010-09-16
    • Related Report
      2012 Final Research Report
  • [Presentation] 『真に価値ある分析科学の発展を目指して』私は研究をしているのか?~効率的バイオマーカー探索プラットフォームの構築~2010

    • Author(s)
      大江知行
    • Organizer
      The 23th Biomedical Analytical Science Symposium(BMAS2010)
    • Place of Presentation
      松島町
    • Year and Date
      2010-07-21
    • Related Report
      2012 Final Research Report
  • [Presentation] バイオマーカー探索を指向したケミカルモディフィコミクスへのアプローチ2010

    • Author(s)
      後藤貴章
    • Organizer
      平成22年度日本薬学会東北支部学術講演会
    • Place of Presentation
      仙台
    • Year and Date
      2010-07-10
    • Related Report
      2012 Final Research Report
  • [Presentation] Novel lipid hydroperoxide-derived oxidized angiotensin peptides as potential modulators of cardiovascular functions2010

    • Author(s)
      Lee, S.H., Takahashi, R., Goto, T., Oe, T.
    • Organizer
      The 12th Asian-Pacific Congress of Clinical Biochemistry
    • Place of Presentation
      Seoul, Korea
    • Related Report
      2012 Final Research Report
  • [Presentation] 非侵襲的サンプリングによる表皮ケラチン分析と酸化修飾解析への応用2010

    • Author(s)
      宮本浩平、李宣和、後藤貴章、大江知行
    • Organizer
      日本分析化学会第59年会
    • Place of Presentation
      仙台
    • Related Report
      2012 Final Research Report 2010 Annual Research Report
  • [Presentation] Novel lipid hydroperoxide-derived oxidized angiotensin peptides as potential biomarkers of cardiovascular disease2010

    • Author(s)
      Seon Hwa Lee
    • Organizer
      The 12th Asian-Pacific Congress of Clinical Biochemistry
    • Place of Presentation
      Seoul, Korea(招待講演)
    • Related Report
      2010 Annual Research Report
  • [Presentation] 内因性アルデヒドに起因する翻訳後修飾:非酵素的なN末端アミノ酸異性化反応の解析2010

    • Author(s)
      梶田遼、後藤貴章、李宣和、大江知行
    • Organizer
      日本分析化学会第59年会
    • Place of Presentation
      仙台
    • Related Report
      2010 Annual Research Report
  • [Presentation] カルボニル化タンパク質の生成機構に関する研究2010

    • Author(s)
      船津啓之、後藤貴章、李宣和、大江知行
    • Organizer
      日本分析化学会第59年会
    • Place of Presentation
      仙台
    • Related Report
      2010 Annual Research Report
  • [Presentation] 脂質過酸化物由来の4-hydroxy-2(E)-nonenalによるangiotensin IIの化学修飾2010

    • Author(s)
      高橋亮、李宣和、後藤貴章、大江知行
    • Organizer
      日本分析化学会第59年会
    • Place of Presentation
      仙台
    • Related Report
      2010 Annual Research Report
  • [Presentation] 標的タンパク質・ペプチド上の化学修飾解析におけるELISA用抗体の活用2010

    • Author(s)
      下道翔平、小島昌太、後藤貴章、李宣和、大江知行
    • Organizer
      日本分析化学会第59年会
    • Place of Presentation
      仙台
    • Related Report
      2010 Annual Research Report
  • [Presentation] 酸化ストレスによる生体高分子の化学修飾機構に関する研究。

    • Author(s)
      李 宣和
    • Organizer
      日本薬学東北支部、第34回東北薬学セミナー
    • Place of Presentation
      仙台
    • Related Report
      2012 Annual Research Report
    • Invited
  • [Presentation] バイオマーカー探索を指向した化学修飾オミクス。

    • Author(s)
      大江 知行
    • Organizer
      第52回日本臨床化学会年次学術集会
    • Place of Presentation
      盛岡
    • Related Report
      2012 Annual Research Report
    • Invited
  • [Presentation] 化学修飾スクリーニングを目的とした修飾ペプチドのフラグメンテーション解析。

    • Author(s)
      吉泉 玲央奈、李 宣和、後藤 貴章、大江 知行
    • Organizer
      第51回日本薬学会東北支部大会
    • Place of Presentation
      青森
    • Related Report
      2012 Annual Research Report
  • [Presentation] 酸化ストレス由来の過酸化脂質分解物によるアンジオテンシンIIの化学修飾解析。

    • Author(s)
      高橋 亮、李 宣和、後藤 貴章、大江 知行
    • Organizer
      みちのく分析科学シンポジウム2012
    • Place of Presentation
      米沢
    • Related Report
      2012 Annual Research Report
  • [Presentation] 非放射性レセプターアッセイによる酸化修飾アンジオテンシンIIとAT1レセプター結合能の評価。

    • Author(s)
      増田 拓朗、李 宣和、後藤 貴章、大江 知行
    • Organizer
      みちのく分析科学シンポジウム2012
    • Place of Presentation
      米沢
    • Related Report
      2012 Annual Research Report
  • [Presentation] Pyridoxamine-mediated transamination of pyruvamide-angiotensin II, a novel angiotensin peptide derived from 4-oxo-2(E)-nonenal.

    • Author(s)
      景 賢淑、李 宣和、後藤 貴章、大江 知行
    • Organizer
      みちのく分析科学シンポジウム2012
    • Place of Presentation
      米沢
    • Related Report
      2012 Annual Research Report
  • [Presentation] タンパク質・ペプチド上のチロシン化学修飾がチロシンリン酸化に与える影響の解析。

    • Author(s)
      新部 陽子、李 宣和、後藤 貴章、大江 知行
    • Organizer
      みちのく分析科学シンポジウム2012
    • Place of Presentation
      米沢
    • Related Report
      2012 Annual Research Report
  • [Book] Lipid peroxide-DNA adducts (Chapter 11) in Penning, T.M. (Ed.) Chemical Carcinogenesis, in El-Deiry, W. (Series Ed.) Current Cancer Research2011

    • Author(s)
      Lee, S.H., Blair, I.A.
    • Publisher
      Springer Science+Business Media LLC
    • Related Report
      2012 Final Research Report
  • [Book] Lipid peroxide-DNA adducts (Chanter 11) in Chemical Carcinogenesis2011

    • Author(s)
      Seon Hwa Lee, Ian A.Blair
    • Publisher
      Springer Science+Business Media LLC
    • Related Report
      2011 Annual Research Report
  • [Remarks] 東北大学大学院薬学研究科臨床分析化学分野へようこそ

    • URL

      http://www.pharm.tohoku.ac.jp/~bunseki/bunseki.html

    • Related Report
      2012 Final Research Report
  • [Remarks] 東北大学大学院薬学研究科 臨床分析化学分野へようこそ

    • URL

      http://www.pharm.tohoku.ac.jp/~bunseki/bunseki.html

    • Related Report
      2012 Annual Research Report
  • [Remarks]

    • URL

      http://www.pharm.tohoku.ac.jp/~bunseki/bunseki.html

    • Related Report
      2011 Annual Research Report
  • [Remarks]

    • URL

      http://www.pharm.tohoku.ac.jp/~bunseki/bunseki.html

    • Related Report
      2010 Annual Research Report

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Published: 2010-08-23   Modified: 2019-07-29  

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