| Project/Area Number |
22590162
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Medical pharmacy
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| Research Institution | Hiroshima International University |
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Principal Investigator |
MORI Nobuhiro 広島国際大学, 薬学部, 准教授 (70412351)
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| Co-Investigator(Kenkyū-buntansha) |
MURAKAMI Teruo 広島国際大学, 薬学部, 教授 (20136055)
YOKOOJI Tomoharu 広島大学, 大学院・医歯薬保健学研究院 (70389120)
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| Project Period (FY) |
2010 – 2012
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| Project Status |
Completed (Fiscal Year 2012)
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| Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2012: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2011: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2010: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | 医療薬剤学 / 妊娠 / 異物排出ポンプ / P-gp / Mrp2 |
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| Research Abstract |
Various endogenous substances including substrates for ABC and SLC transporters expressed in the kidney are excreted into urine. In this study, the first urine in the morning (total 15 samples) and urine in the daytime (total 4 days, 18 samples) were collected at intervals from a young healthy woman before and after childbirth, to examine possible interactions of hydrophobic urine components (methanol extracts) with P-glycoprotein (P-gp) and multidrug resistance-associated proteins (MRPs). Four urine samples out of 12 collected before childbirth and one sample out of three obtained after childbirth suppressed P-gp function significantly. No relationship was observed between the P-gp inhibitory potencies of urine extracts and the timing of childbirth. The daytime urine samples showed a clear circadian rhythm, in which three first urine samples in the morning out of four showed the greater P-gp inhibitory potencies than other samples. Interaction of urine extracts with MRPs was no detected in Caco-2 cells. In conclusion, urine contains various endogenous P-gp substrates/inhibitor(s), and the concentration of endogenous P-gp inhibitor(s) was higher in the first urine in the morning. Pregnancy and lactation did not affect the P-gp inhibitory potencies of urine extracts.
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